Zyx (BC054775) Mouse Tagged ORF Clone

CAT#: MR208545

  • TrueORF®

Zyx (Myc-DDK-tagged) - Mouse zyxin (cDNA clone MGC:65721 IMAGE:6808149)


  "BC054775" in other vectors (4)


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Reconstitution Protocol

USD 320.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Zyx
Synonyms 9530098H06Rik|R75157
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208545 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCCCCCGCCCGCCTCCCGCGATCTCCGTCTCCGTCTCGGCCCCCGCGTTTTACGCCCCGCAGA
AGAAGTTCGCCCCGGTTGTGGCCCCAAAGCCCAAAGTGAATCCTTTCCGGCCTGGGGACAGCGAGCCTCC
TGTAGCAGCCGGGGCCCAAAGAGCGCAGATGGGTCGGGTGGGCGAGATCCCACCACCACCCCCGGAAGAC
TTTCCTTTGCCCCCTCCTCCCCTTATTGGGGAGGGCGACGACTCAGAGGGTGCCCTGGGAGGTGCCTTCC
CACCTCCACCTCCCCCGATGATCGAGGAACCATTCCCCCCTGCTCCTCTGGAGGAGGACATCTTCCCCTC
CCCTCCACCTCCACTGGAGGAGGAGGGAGGGCCTGAGGCCCCTACCCAGCTCCCACCGCAGGTATCATCC
GGATATGTACCCCCACCAGTTGCCACTCCATTTGTTCCCAAGCCTAGTACCAAACCTGCCCCTGGGGGCA
CAGCACCCTTGCCTCCTTGGAAGACCCCTTCTAGCTCCCAGCCACCACCTCAGCCGCAGGCCAAGCCTCA
GGTCCAGCTCCATGTCCAGCCTCAGGCCAAGCCCCATGTCCAACCCCAGCCTGTGTCTTCTGCTAATACA
CAGCCCCGGGGTCCCCTTTCTCAGGCACCAACTCCAGCACCTAAGTTTGCTCCAGTGGCTCCTAAATTTA
CTCCCGTGGTTTCCAAGTTCAGCCCTGGTGCTCCAAGTGGACCTGGGCCACAGCCCAATCAAAAAATGGT
GCCTCCGGATGCTCCTTCTTCTGTGAGCACAGGCTCCCCTCAGCCCCCTAGCTTCACCTATGCTCAGCAG
AAGGAGAAGCCCCTAGTTCAAGAGAAGCAGCACCCACAGCCTCCACCAGCTCAAAACCAAAACCAGGTAC
GCTCTCCTGGAGGCCCAGGCCCCTTGACCCTGAAGGAGGTAGAGGAGTTGGAGCAGCTGACCCAGCAGCT
GATGCAGGACATGGAACACCCTCAGAGGCAGAGCGTGGCAGTGAATGAGTCCTGTGGCAAATGCAATCAG
CCACTGGCCCGTGCACAGCCTGCGGTTCGTGCACTGGGACAACTGTTCCACATCACCTGCTTCACTTGCC
ATCAGTGTCAGCAGCAGCTGCAGGGACAGCAGTTCTATAGCCTGGAGGGAGCACCATATTGTGAGGGCTG
CTACACCGACACTTTGGAGAAGTGCAACACCTGTGGGCAGCCCATCACTGACCGCATGCTGAGGGCCACT
GGCAAAGCCTACCACCCACAGTGCTTCACCTGTGTGGTCTGCGCCTGTCCCCTGGAGGGCACCTCCTTCA
TTGTGGACCAGGCCAATCAGCCCCACTGTGTCCCTGACTATCACAAGCAATACGCTCCAAGGTGCTCCGT
CTGCTCGGAGCCAATCATGCCTGAGCCTGGCCGAGACGAGACTGTGCGAGTAGTGGCGCTGGATAAGAAC
TTTCATATGAAGTGTTACAAGTGTGAGGACTGTGGGAAACCTCTGTCCATTGAGGCAGATGACAACGGCT
GTTTCCCTCTGGATGGCCACGTCCTTTGTCGGAAGTGCCACTCCGCTAGAGCCCAGACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208545 protein sequence
Red=Cloning site Green=Tags(s)

MAAPRPPPAISVSVSAPAFYAPQKKFAPVVAPKPKVNPFRPGDSEPPVAAGAQRAQMGRVGEIPPPPPED
FPLPPPPLIGEGDDSEGALGGAFPPPPPPMIEEPFPPAPLEEDIFPSPPPPLEEEGGPEAPTQLPPQVSS
GYVPPPVATPFVPKPSTKPAPGGTAPLPPWKTPSSSQPPPQPQAKPQVQLHVQPQAKPHVQPQPVSSANT
QPRGPLSQAPTPAPKFAPVAPKFTPVVSKFSPGAPSGPGPQPNQKMVPPDAPSSVSTGSPQPPSFTYAQQ
KEKPLVQEKQHPQPPPAQNQNQVRSPGGPGPLTLKEVEELEQLTQQLMQDMEHPQRQSVAVNESCGKCNQ
PLARAQPAVRALGQLFHITCFTCHQCQQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGQPITDRMLRAT
GKAYHPQCFTCVVCACPLEGTSFIVDQANQPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKN
FHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHSARAQT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC054775
ORF Size 1599 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC054775, AAH54775
RefSeq Size 2323 bp
RefSeq ORF 1601 bp
Locus ID 22793
MW 57 kDa

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