Zyx (BC054775) Mouse Tagged ORF Clone

CAT#: MR208545

Zyx (Myc-DDK-tagged) - Mouse zyxin (cDNA clone MGC:65721 IMAGE:6808149)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zyx
• Synonyms: 9530098H06Rik|R75157
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208545 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCCCCGCCCGCCTCCCGCGATCTCCGTCTCCGTCTCGGCCCCCGCGTTTTACGCCCCGCAGA
  AGAAGTTCGCCCCGGTTGTGGCCCCAAAGCCCAAAGTGAATCCTTTCCGGCCTGGGGACAGCGAGCCTCC
  TGTAGCAGCCGGGGCCCAAAGAGCGCAGATGGGTCGGGTGGGCGAGATCCCACCACCACCCCCGGAAGAC
  TTTCCTTTGCCCCCTCCTCCCCTTATTGGGGAGGGCGACGACTCAGAGGGTGCCCTGGGAGGTGCCTTCC
  CACCTCCACCTCCCCCGATGATCGAGGAACCATTCCCCCCTGCTCCTCTGGAGGAGGACATCTTCCCCTC
  CCCTCCACCTCCACTGGAGGAGGAGGGAGGGCCTGAGGCCCCTACCCAGCTCCCACCGCAGGTATCATCC
  GGATATGTACCCCCACCAGTTGCCACTCCATTTGTTCCCAAGCCTAGTACCAAACCTGCCCCTGGGGGCA
  CAGCACCCTTGCCTCCTTGGAAGACCCCTTCTAGCTCCCAGCCACCACCTCAGCCGCAGGCCAAGCCTCA
  GGTCCAGCTCCATGTCCAGCCTCAGGCCAAGCCCCATGTCCAACCCCAGCCTGTGTCTTCTGCTAATACA
  CAGCCCCGGGGTCCCCTTTCTCAGGCACCAACTCCAGCACCTAAGTTTGCTCCAGTGGCTCCTAAATTTA
  CTCCCGTGGTTTCCAAGTTCAGCCCTGGTGCTCCAAGTGGACCTGGGCCACAGCCCAATCAAAAAATGGT
  GCCTCCGGATGCTCCTTCTTCTGTGAGCACAGGCTCCCCTCAGCCCCCTAGCTTCACCTATGCTCAGCAG
  AAGGAGAAGCCCCTAGTTCAAGAGAAGCAGCACCCACAGCCTCCACCAGCTCAAAACCAAAACCAGGTAC
  GCTCTCCTGGAGGCCCAGGCCCCTTGACCCTGAAGGAGGTAGAGGAGTTGGAGCAGCTGACCCAGCAGCT
  GATGCAGGACATGGAACACCCTCAGAGGCAGAGCGTGGCAGTGAATGAGTCCTGTGGCAAATGCAATCAG
  CCACTGGCCCGTGCACAGCCTGCGGTTCGTGCACTGGGACAACTGTTCCACATCACCTGCTTCACTTGCC
  ATCAGTGTCAGCAGCAGCTGCAGGGACAGCAGTTCTATAGCCTGGAGGGAGCACCATATTGTGAGGGCTG
  CTACACCGACACTTTGGAGAAGTGCAACACCTGTGGGCAGCCCATCACTGACCGCATGCTGAGGGCCACT
  GGCAAAGCCTACCACCCACAGTGCTTCACCTGTGTGGTCTGCGCCTGTCCCCTGGAGGGCACCTCCTTCA
  TTGTGGACCAGGCCAATCAGCCCCACTGTGTCCCTGACTATCACAAGCAATACGCTCCAAGGTGCTCCGT
  CTGCTCGGAGCCAATCATGCCTGAGCCTGGCCGAGACGAGACTGTGCGAGTAGTGGCGCTGGATAAGAAC
  TTTCATATGAAGTGTTACAAGTGTGAGGACTGTGGGAAACCTCTGTCCATTGAGGCAGATGACAACGGCT
  GTTTCCCTCTGGATGGCCACGTCCTTTGTCGGAAGTGCCACTCCGCTAGAGCCCAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208545 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPRPPPAISVSVSAPAFYAPQKKFAPVVAPKPKVNPFRPGDSEPPVAAGAQRAQMGRVGEIPPPPPED
  FPLPPPPLIGEGDDSEGALGGAFPPPPPPMIEEPFPPAPLEEDIFPSPPPPLEEEGGPEAPTQLPPQVSS
  GYVPPPVATPFVPKPSTKPAPGGTAPLPPWKTPSSSQPPPQPQAKPQVQLHVQPQAKPHVQPQPVSSANT
  QPRGPLSQAPTPAPKFAPVAPKFTPVVSKFSPGAPSGPGPQPNQKMVPPDAPSSVSTGSPQPPSFTYAQQ
  KEKPLVQEKQHPQPPPAQNQNQVRSPGGPGPLTLKEVEELEQLTQQLMQDMEHPQRQSVAVNESCGKCNQ
  PLARAQPAVRALGQLFHITCFTCHQCQQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGQPITDRMLRAT
  GKAYHPQCFTCVVCACPLEGTSFIVDQANQPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKN
  FHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHSARAQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC054775
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC054775, AAH54775
• RefSeq Size: 2323 bp
• RefSeq ORF: 1601 bp
• Locus ID: 22793
• MW: 57 kDa