Grb7 (NM_010346) Mouse Tagged ORF Clone

CAT#: MR208570

Grb7 (Myc-DDK-tagged) - Mouse growth factor receptor bound protein 7 (Grb7)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grb7
• Synonyms: mKIAA4028
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208570 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGGATCTGAGCCCGACTCATCTCAGCAGCTCCCCAGAAGATGTGTGCCCAACTCCTGCTACCC
  CTCCTGAGACTCCTCCGCCCCCTGATAACCCTCCGCCAGGGGATGTGAAGCGGTCGCAGCCTTTGCCCAT
  CCCCAGCAGCAGGAAACTTCGAGAAGAGGAGTTTCAGGCAACCTCTCTGCCTTCCATCCCCAACCCCTTC
  CCTGAGCTCTGCAGCCCACCTTCACAGAAACCCATTCTTGGTGGTTCCTCCGGTGCAAGGGGGTTGCTTC
  CTCGAGACTCCAGCCGCCTCTGTGTGGTGAAGGTGTACAGTGAGGATGGGGCCTGCCGGTCTGTGGAGGT
  GGCAGCGGGCGCCACAGCTCGTCACGTGTGTGAGATGCTGGTACAACGAGCTCACGCCCTGAGCGACGAG
  AGCTGGGGACTAGTGGAATCCCACCCCTACCTGGCACTGGAGCGGGGTCTGGAGGACCATGAATTTGTGG
  TGGAAGTGCAGGAGGCCTGGCCTGTGGGTGGAGACAGCCGCTTCATCTTTCGTAAAAACTTCGCCAAGTA
  TGAACTATTCAAGAGCCCCCCACACACCCTGTTCCCAGAAAAGATGGTCTCGAGCTGTCTGGATGCACAA
  ACAGGCATATCCCATGAAGACCTCATCCAGAACTTCCTGAACGCTGGCAGCTTCCCTGAGATCCAGGGCT
  TCCTGCAGCTGCGGGGATCAGGCCGGGGGTCAGGTCGAAAGCTTTGGAAACGTTTCTTCTGCTTTCTGCG
  TCGATCTGGCCTCTACTACTCTACCAAGGGTACCTCCAAGGACCCCAGACACCTACAGTATGTGGCAGAT
  GTGAATGAGTCCAATGTCTATGTGGTGACCCAGGGCCGCAAGCTGTATGGGATGCCCACTGACTTCGGCT
  TCTGTGTCAAGCCCAACAAGCTTCGAAACGGCCACAAGGGGCTCCACATCTTCTGCAGTGAGGATGAGCA
  GAGTCGGACCTGCTGGCTGGCTGCCTTCCGGCTCTTCAAGTACGGGGTACAGCTATATAAGAATTATCAG
  CAGGCCCAGTCTCGTCACCTGCGCCTATCCTATTTGGGGTCTCCACCCTTGAGGAGCGTCTCAGACAATA
  CCCTAGTGGCTATGGACTTCTCTGGCCATGCGGGGCGTGTCATTGATAACCCCCGGGAAGCTCTGAGTGC
  CGCCATGGAGGAGGCCCAGGCCTGGAGGAAGAAGACAAACCACCGTCTGAGCCTGCCCACCACATGCTCT
  GGCTCGAGCCTCAGCGCAGCCATTCATCGCACCCAGCCCTGGTTTCATGGACGCATCTCTCGGGAGGAGA
  GCCAGCGGCTAATTGGACAGCAGGGCCTGGTGGATGGTGTGTTCCTGGTCCGGGAGAGCCAGAGGAACCC
  ACAGGGCTTTGTCCTGTCCTTGTGCCATCTGCAGAAAGTCAAGCATTATCTCATTTTGCCAAGCGAAGAT
  GAAGGTTGCCTTTACTTCAGCATGGATGAGGGCCAGACCCGTTTCACAGACCTGCTGCAGCTGGTAGAAT
  TCCACCAGCTGAACCGAGGCATCCTGCCCTGCCTGCTGCGCCACTGCTGTGCCCGTGTGGCCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208570 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELDLSPTHLSSSPEDVCPTPATPPETPPPPDNPPPGDVKRSQPLPIPSSRKLREEEFQATSLPSIPNPF
  PELCSPPSQKPILGGSSGARGLLPRDSSRLCVVKVYSEDGACRSVEVAAGATARHVCEMLVQRAHALSDE
  SWGLVESHPYLALERGLEDHEFVVEVQEAWPVGGDSRFIFRKNFAKYELFKSPPHTLFPEKMVSSCLDAQ
  TGISHEDLIQNFLNAGSFPEIQGFLQLRGSGRGSGRKLWKRFFCFLRRSGLYYSTKGTSKDPRHLQYVAD
  VNESNVYVVTQGRKLYGMPTDFGFCVKPNKLRNGHKGLHIFCSEDEQSRTCWLAAFRLFKYGVQLYKNYQ
  QAQSRHLRLSYLGSPPLRSVSDNTLVAMDFSGHAGRVIDNPREALSAAMEEAQAWRKKTNHRLSLPTTCS
  GSSLSAAIHRTQPWFHGRISREESQRLIGQQGLVDGVFLVRESQRNPQGFVLSLCHLQKVKHYLILPSED
  EGCLYFSMDEGQTRFTDLLQLVEFHQLNRGILPCLLRHCCARVAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010346
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010346.1, NM_010346.2, NP_034476.1
• RefSeq Size: 2413 bp
• RefSeq ORF: 1608 bp
• Locus ID: 14786
• MW: 60 kDa