Fmo2 (NM_018881) Mouse Tagged ORF Clone

CAT#: MR208580

Fmo2 (Myc-DDK-tagged) - Mouse flavin containing monooxygenase 2 (Fmo2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fmo2
• Synonyms: 2310008D08Rik; 2310042I22Rik; AW107733
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208580 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGAAGGTTGTGGTGATTGGAGCAGGGGTCAGTGGCCTAATCTCTCTGAAGTGCTGCGTGGACG
  AGGGTCTGGAGCCCACCTGCTTCGAAAGGACTGAAGATATAGGGGGACTGTGGAGATTCAAAGAAAACGT
  GGAAGATGGCCGCGCTAGCATCTACCGCTCTGTCATTACCAACACTAGCAAAGAAATGTCCTGTTTCAGT
  GACTTCCCGATGCCGGAGGACTTCCCCAACTTCCTGCACAACTCTAAACTCCTGGAATATTTCAGGATCT
  TTGCCAAGAAATTTGATCTCCTAAAATATATTCAGTTTCAGACAACTGTCATTAGTGTGAAAAAACGCCC
  GGATTTCGCATCTTCTGGGCAATGGGAAGTTTATACTCAGAGCAACGGAAAGGAGCAACGCACTGTCTTT
  GACGCTGTTATGGTTTGCAGCGGCCATCACATCCAGCCTCACCTGCCACTCAAGTCATTCCCAGGTATTG
  AGAGGTTCCGAGGCCAGTATTTCCATAGCCGCGAATACAAGCACCCAGTGGGGTTTGAGGGGAAACGCAT
  CCTGGTGGTTGGAATAGGAAATTCAGCTGCAGATATTGCCTCTGAACTGAGTAAGACGGCCGCACAGGTG
  TTTGTCAGCACCAGACATGGATCCTGGGTCATGAGTCGAATCTCTGAAGATGGCTACCCTTGGGACATGG
  TGTTCCACACTAGATTTAGCTCCATGCTCCGAAATGTCCTGCCCCGCACGGTCGTCAAGTGGATGATGGA
  ACAACAGATGAATCGATGGTTCAACCATGAAAATTATGGTCTGGTGCCTCAGAACAAATACCTTATGAAA
  GAACCTGTGCTAAACGACGACCTCCCCAGCCGCCTGCTCTACGGAGCCATCAAAGTAAAAACACGGGTGA
  AGGAGCTCACGGAGACAGCTGTGGTCTTCGAGGATGGCACGGTGGAGGAAGACGTGGATATCATTGTCTT
  TGCCACGGGGTATACGTTCTCTTTTTCTTTCCTGGAAGACTCACTTGTTAAAGTGGAGGATAATAGGGTT
  TCCCTGTACAAAGCCATGTTTCCCCCTCACCTGGAGAAGCCAACCCTTGCGTGCATAGGTCTCATCCAGC
  CTCTAGGCTCCATCTTCCCAACCGTAGAGCTTCAGGCGCGCTGGGCAACAAGAGTTTTCAAAGGCTTGTG
  TAGCTTGCCTTCGGAGACGACTATGATGGCAGACATTGTTGAAAGAAATGAAAAAAGAGTTAACCTGTTT
  GGGAAAAGCCAGAGTCAGATACTGCAGACCAACTACGTTGACTACCTGGATGAACTCGCCTTGGAGATAG
  GTGCAAAGCCAGATTTCGTCTCTCTCTTTTTCAAAGACCCTAAACTGGCTGTGAAACTCTACTTCGGACC
  CTGTAATTCCTACCAGTACCGCCTGGTTGGACCTGGGCAGTGGGAAGGAGCCAGGAACGCCATCCTCACC
  CAGAAGCAGAGGATCCTGAAACCCTTAAAGACCCGGACTCTCCAGTCCTCTGATAGTGCCCCAGTGTCTT
  TCCTGCTCAAGATCCTGGGGCTGCTCGCTGTTGTTCTGGCCTTCTTTTTCCAACTTCAGGGGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208580 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKKVVVIGAGVSGLISLKCCVDEGLEPTCFERTEDIGGLWRFKENVEDGRASIYRSVITNTSKEMSCFS
  DFPMPEDFPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVISVKKRPDFASSGQWEVYTQSNGKEQRTVF
  DAVMVCSGHHIQPHLPLKSFPGIERFRGQYFHSREYKHPVGFEGKRILVVGIGNSAADIASELSKTAAQV
  FVSTRHGSWVMSRISEDGYPWDMVFHTRFSSMLRNVLPRTVVKWMMEQQMNRWFNHENYGLVPQNKYLMK
  EPVLNDDLPSRLLYGAIKVKTRVKELTETAVVFEDGTVEEDVDIIVFATGYTFSFSFLEDSLVKVEDNRV
  SLYKAMFPPHLEKPTLACIGLIQPLGSIFPTVELQARWATRVFKGLCSLPSETTMMADIVERNEKRVNLF
  GKSQSQILQTNYVDYLDELALEIGAKPDFVSLFFKDPKLAVKLYFGPCNSYQYRLVGPGQWEGARNAILT
  QKQRILKPLKTRTLQSSDSAPVSFLLKILGLLAVVLAFFFQLQGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018881
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018881.1, NM_018881.2, NM_018881.3, NP_061369.2
• RefSeq Size: 3336 bp
• RefSeq ORF: 1608 bp
• Locus ID: 55990
• MW: 61 kDa
• Gene Summary: This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Shows catalytic activity towards methimazole, thiourea, trimethylamine, and the insecticide phorate. [UniProtKB/Swiss-Prot Function]