Leprel2 (BC003726) Mouse Tagged ORF Clone

CAT#: MR208685

Leprel2 (Myc-DDK-tagged) - Mouse leprecan-like 2 (cDNA clone MGC:5777 IMAGE:3497610)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Leprel2
• Synonyms: BC016431|Grcb|Leprel2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208685 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGAAGACATGGCTAAGTACAGAAGGATGTCTGCAATCCGACCCCAGAGCTTCCGGGACCTGGTGA
  CGCCCCTATACTGGGCAGCTTATGACACTGGCCTGGAGCTTCTGGAGCAACGGGAGGCAGCACTGGCTCT
  ACCCCAACTAGAGGAGGCCCTGCAGGGGAGCCTGGCCCACATGGAGAGCTGCCGTGCTGCCTGTGAGGGG
  CCTGAGGAGCACCAAGGGGCTGAAGAAGAGGGAGAAGGGAGCCAGGGAGGCCTGTATGAAGCCATTGCAG
  GACACTGGATACGGGTTCTGCAGTGCAGGCAGCACTGTGTGGCAGACACGGCCACCCGTCCTGGTCGCAG
  CTTCCCTGTCCAGGACTTCCTCCTCAGCCAGCTGAGACGGCTGCACGAGGCGTATGCTCAGGTGGGGAAC
  ATGTCTCAGGCCATGGAAAATGTCCTGAGTGTCCTGCTCTTCTACCCAGAGGATGAGGCTGCCAAAAAGG
  CTCTGAACCAGTACCAAACTCAGTTGGGAGAGCCAAGACCTGACCTGGGACCACGAGAGGACATCCAGCG
  GTTCATCCTTCGCTCCCTCGGAGAGAAGAGACAGTTATATTACGCCATGGAGCATCTGGGCACTAGCTTC
  AAGGACCCGGATTCTTGGACCCCGGAGGCTCTCATCCCTAAGGCACTGAGAGAGAGGCTCAGAGAGGATC
  AAGAGAAGAAGCCCTGGGACCATCAGCCTCCACAGCAGAAGCCCTTGGCTCACTGGAAGGATGCCCTTCT
  GATGGAGGGTGTGACCCTCACGCAGGACGCCCAGCAGCTGAATGGGTCGGAGCGAGCTGTCTTAGATGGG
  CTGCTAACTTCAGCCGAGTGTGGGGTTCTGCTGCAGCTGGCCAAGGATGCAGCACAGGCTGGAGCCAGGT
  CAGGCTACCGTGGCCGCCGCTCTCCTCATAGTCCTCATGAACGCTTTGAGGGGCTCACGGTGCTCAAGGC
  TGCTCAGCTGGCCCGGGCAGGGACTGTGGGCAGGCCAGGCGCTAAGCTGCTTCTGGAGGTAAGTGAGCGA
  GTGCGGACTTTGACCCAGGCCTACTTCTCCCCGGAACGGCCACTGCATCTTTCCTTCACCCACCTGGTGT
  GCCGAAGTGCCATAGAAGGTGAACAAGAGCAGCGTATGGACCTGAGTCACCCGGTTCATGCAGACAACTG
  TGTCCTGGACCCCGACACTGGCGAATGCTGGCGGGAGCCCCCAGCCTACACCTATCGAGACTATAGTGGA
  CTCCTCTACCTCAATGATGACTTCAAGGGAGGCGACCTGTTCTTCACGCAGCCCAACGCCCTCACTGTCA
  CGGCTCAGGTCCGTCCTCGGTGTGGGCGCCTTGTGGCCTTCAGCTCTGGTGGTGAGAATCCCCATGGTGT
  ATGGGCTGTGACTCGGGGACGGCGCTGTGCCCTAGCACTGTGGCACACGTGGGCACCTGAGCACAGTGAA
  CAGGAGTGGACAGAAGCCAAAGAGCTGCTGCAGGAGGAAGAGGAGGAAGAAGAGGAGGAAGACATTCTCA
  GCAGAGACCCTTCCCCAGAACCCCCAAGTCACAAGCTTCAGCGAGTCCAGGAGAAAGCTGGGAAGCCCCG
  CCGGGTCCGGGTCCGAGAGGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208685 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MREDMAKYRRMSAIRPQSFRDLVTPLYWAAYDTGLELLEQREAALALPQLEEALQGSLAHMESCRAACEG
  PEEHQGAEEEGEGSQGGLYEAIAGHWIRVLQCRQHCVADTATRPGRSFPVQDFLLSQLRRLHEAYAQVGN
  MSQAMENVLSVLLFYPEDEAAKKALNQYQTQLGEPRPDLGPREDIQRFILRSLGEKRQLYYAMEHLGTSF
  KDPDSWTPEALIPKALRERLREDQEKKPWDHQPPQQKPLAHWKDALLMEGVTLTQDAQQLNGSERAVLDG
  LLTSAECGVLLQLAKDAAQAGARSGYRGRRSPHSPHERFEGLTVLKAAQLARAGTVGRPGAKLLLEVSER
  VRTLTQAYFSPERPLHLSFTHLVCRSAIEGEQEQRMDLSHPVHADNCVLDPDTGECWREPPAYTYRDYSG
  LLYLNDDFKGGDLFFTQPNALTVTAQVRPRCGRLVAFSSGGENPHGVWAVTRGRRCALALWHTWAPEHSE
  QEWTEAKELLQEEEEEEEEEDILSRDPSPEPPSHKLQRVQEKAGKPRRVRVREEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003726
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003726, AAH03726
• RefSeq Size: 2289 bp
• RefSeq ORF: 1637 bp
• Locus ID: 14789
• MW: 61.7 kDa