Zfp451 (BC019546) Mouse Tagged ORF Clone

CAT#: MR208860

Zfp451 (Myc-DDK-tagged) - Mouse zinc finger protein 451 (cDNA clone MGC:28438 IMAGE:4038791)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp451
• Synonyms: COASTER
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208860 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACCCAGGGCCGGAGATAATAGAATCTGTCCCACCAGCTGGGCCCGAGGCATCAGAGTCTACCA
  CGGATGAAAACGAAGATGACATTCAGTTTGTTAGTGAAGGACCGTTAAGACCCGTTTTGGAATACATTGA
  TCTAGTCAGCAGTGATGATGAAGAGCCTAGTACCTCTCACAGTGATAGAATGCCTGAATCTAAAGTGCCA
  TCCTCTGAGAATCATCGCCCAGAAATGTGCTCTAGCTGCAGTGTTCCTCTTCCCATTGGAGACAGCAGCT
  CCTCCTCTGGGAGTTGTGCCAACAGCCCACAAAGGATAGTTTCTCAACCTTCTTCTGTTGAGAACCCATT
  GGAGAACCAGAAAAATGATCACAATAATTCCGATGTTAGTGTCTCTGAAAAAGAGACACTTAAACCACAA
  AGTAATCAGACTCTGCCTTCAGCATCACTTCTGGGCTCCCAGGAATCTTTGGCTTTTTCTGAGGTCAAAG
  AGGATTTACCTGGCACGATGTCTTCAGCTTCACAGAGTGGACAGGATGCCATCCTGTATCTCCAGACACA
  AGTGGCTGAGATGTCCCGAGTCATACGTGATCTGCAGTCCAGGAGTTGTTTTAGATTTCATCATTCTAGG
  CCAAGTGAGAGCTCGTCAGTTCCTTGGGATATCTCCAGCTCTAAGGAGGACAGTTTATCCACAGTTGAAG
  AGGAGTCTGACTGCAAATCTCCGTCAGCTGATGACAAAGGGCAGCCACCTGACCCCATGCAGTCTAGTTT
  TACCGGTCTTTTGAAGAGAATGGAACAAAGGGGTGTTATAAAGAGGGTGACATTCCAGTCTGAAGCAGAA
  ACCTGTGAGGGCAAGCCTGATTGGATGACCTCTAAAAAACGCTTGGTTCCTCCACTGCATCCACTTTTGA
  GAATTGCCACCACTGAAGTTTTTAAAGACCCTGCTGATTGCCATCCTTCTTCCTTCATGGGACACAGGGT
  TTATCCTGTGGCCAAGGACACATCTCCTTTCCAGCCAAATCCACCAGCTGAGGGACCCATCATAGATGTA
  TTAGAACATAGCAAAAGAGGAAACACAGCATCGCCTTTAGACTCTACCTCAAAAGAAATGGAGGTCATGG
  GTTGCAGGTTCTACCATGCTGCTTCCATTGCAGCCCGGGCTGCTAGCTACATGGCCTATATGACTCAGTA
  TCAACGTAAACTCTGGGAAGACATGGAAGATCTGGTTCATGACCCGGAGTTTGATCGTGGGAAAGCAAGG
  TGTATAATATCTGATGGCATGGATGCAGGACTTTGGCAGCTCTGTACCACTAGGGACATAATGGACTCTG
  TAGTCAGAGTTATGGCCATGGCAGTAGACTATAGAAGACAGGCCTGGCTCCGACTAACATCTCTCACTAA
  GAAAACCCAGGAGAAGATCTTACACTTGCCCTTTGATGGTACATCTCTTTTTGGACAAGATGTGAATGCT
  ATTGTTGCAGAAGACAATAGTATTAAAGAAAATGAATATAAGGATCACAATAAATACTATACTCAGCATC
  GGTACTTTTATAGTCATGATCAGAAAGCACATTATCACAACAGAGGGTACTCCAAAGGGGATTGGTACAA
  ACCCCGGAATCACCCCTATAGATACAGAAAAAAGGGAGACTCTCCAGAACGGCATGGGTATAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208860 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDPGPEIIESVPPAGPEASESTTDENEDDIQFVSEGPLRPVLEYIDLVSSDDEEPSTSHSDRMPESKVP
  SSENHRPEMCSSCSVPLPIGDSSSSSGSCANSPQRIVSQPSSVENPLENQKNDHNNSDVSVSEKETLKPQ
  SNQTLPSASLLGSQESLAFSEVKEDLPGTMSSASQSGQDAILYLQTQVAEMSRVIRDLQSRSCFRFHHSR
  PSESSSVPWDISSSKEDSLSTVEEESDCKSPSADDKGQPPDPMQSSFTGLLKRMEQRGVIKRVTFQSEAE
  TCEGKPDWMTSKKRLVPPLHPLLRIATTEVFKDPADCHPSSFMGHRVYPVAKDTSPFQPNPPAEGPIIDV
  LEHSKRGNTASPLDSTSKEMEVMGCRFYHAASIAARAASYMAYMTQYQRKLWEDMEDLVHDPEFDRGKAR
  CIISDGMDAGLWQLCTTRDIMDSVVRVMAMAVDYRRQAWLRLTSLTKKTQEKILHLPFDGTSLFGQDVNA
  IVAEDNSIKENEYKDHNKYYTQHRYFYSHDQKAHYHNRGYSKGDWYKPRNHPYRYRKKGDSPERHGYKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC019546
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019546, AAH19546
• RefSeq Size: 1928 bp
• RefSeq ORF: 1679 bp
• Locus ID: 98403
• MW: 62.7 kDa