Fzd2 (NM_020510) Mouse Tagged ORF Clone

CAT#: MR208974

Fzd2 (Myc-DDK-tagged) - Mouse frizzled homolog 2 (Drosophila) (Fzd2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fzd2
• Synonyms: AL033370; AW456835; Fz10; Fzd10; Mfz10; Mfz10a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208974 representing NM_020510
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCCCGCAGCGCCCTGCCCCGCAGCGCCCTGCCCCGCCTGCTGCTGCCACTGCTGCTGCTGCCGG
  CCGCCGGACCGGCCCAGTTCCACGGGGAGAAGGGCATCTCCATCCCGGACCACGGCTTCTGCCAGCCCAT
  CTCCATCCCGCTGTGCACGGACATCGCCTACAACCAGACCATCATGCCCAACCTTCTTGGCCACACGAAC
  CAGGAAGACGCGGGCCTGGAGGTGCATCAGTTCTACCCGCTGGTGAAGGTGCAGTGCTCGCCCGAGCTGC
  GCTTCTTCCTGTGCTCCATGTACGCGCCGGTGTGCACAGTGCTGGAGCAGGCCATCCCGCCGTGCCGCTC
  CATCTGCGAGCGCGCGCGCCAAGGCTGCGAGGCGCTCATGAACAAGTTCGGCTTCCAATGGCCCGAGCGC
  CTCCGCTGCGAGCATTTCCCGCGTCACGGCGCGGAGCAGATCTGCGTGGGCCAGAACCACTCGGAGGACG
  GAGCTCCTGCGCTACTCACCACCGCGCCACCTTCTGGGCTGCAGCCCGGCGCGGGTGGCACCCCGGGCGG
  CCCTGGCGGTGGTGGCTCGCCACCGCGTTACGCCACTCTGGAGCACCCTTTCCACTGTCCCCGCGTCCTC
  AAGGTGCCGTCCTATCTCAGCTATAAGTTTCTGGGTGAGCGCGATTGTGCCGCGCCCTGCGAGCCCGCAC
  GGCCCGACGGCTCTATGTTCTTCTCGCAAGAGGAGACTCGTTTTGCCCGTCTCTGGATCCTCACATGGTC
  GGTGTTGTGCTGCGCTTCCACTTTCTTCACGGTCACCACCTATTTAGTGGACATGCAGCGATTTCGCTAC
  CCAGAGCGGCCCATCATCTTTCTGTCCGGCTGCTACACCATGGTGTCAGTGGCCTACATTGCGGGCTTCG
  TTCTCCAGGAGCGCGTGGTATGCAATGAGCGCTTCTCAGAGGACGGTTATCGCACGGTGGTGCAGGGCAC
  TAAGAAAGAAGGCTGCACTATACTCTTCATGATGCTCTACTTCTTCAGCATGGCCAGCTCCATCTGGTGG
  GTGATTCTGTCCCTCACCTGGTTCCTGGCAGCCGGAATGAAGTGGGGCCACGAGGCCATCGAGGCCAATT
  CGCAGTACTTCCACCTGGCCGCCTGGGCCGTGCCGGCCGTCAAAACCATCACCATCTTGGCCATGGGCCA
  GATCGACGGCGACCTGCTGAGCGGCGTGTGCTTCGTGGGCCTCAATAGCCTGGACCCGCTGCGGGGCTTC
  GTGCTGGCGCCGCTCTTCGTATACCTGTTCATCGGTACATCCTTCCTGCTGGCCGGCTTCGTGTCACTCT
  TCCGCATCCGCACCATCATGAAGCACGACGGCACCAAGACGGAGAAGCTGGAGAGGCTCATGGTGCGCAT
  TGGCGTCTTCTCGGTGCTCTACACGGTACCGGCCACCATCGTCATCGCCTGCTACTTCTATGAGCAGGCC
  TTCCGCGAGCACTGGGAGCGCTCCTGGGTAAGCCAGCACTGCAAGAGCCTAGCCATCCCCTGCCCGGCCC
  ACTACACGCCCCGCATGTCGCCCGACTTCACAGTCTACATGATCAAATACCTCATGACGCTCATCGTGGG
  CATCACGTCGGGCTTCTGGATCTGGTCCGGCAAGACACTGCACTCGTGGAGGAAGTTCTACACTCGTCTC
  ACCAACAGCCGGCATGGCGAGACCACTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208974 representing NM_020510
  Red=Cloning site Green=Tags(s)
  
  MRARSALPRSALPRLLLPLLLLPAAGPAQFHGEKGISIPDHGFCQPISIPLCTDIAYNQTIMPNLLGHTN
  QEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSICERARQGCEALMNKFGFQWPER
  LRCEHFPRHGAEQICVGQNHSEDGAPALLTTAPPSGLQPGAGGTPGGPGGGGSPPRYATLEHPFHCPRVL
  KVPSYLSYKFLGERDCAAPCEPARPDGSMFFSQEETRFARLWILTWSVLCCASTFFTVTTYLVDMQRFRY
  PERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVVQGTKKEGCTILFMMLYFFSMASSIWW
  VILSLTWFLAAGMKWGHEAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNSLDPLRGF
  VLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLERLMVRIGVFSVLYTVPATIVIACYFYEQA
  FREHWERSWVSQHCKSLAIPCPAHYTPRMSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRL
  TNSRHGETTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020510
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020510.1, NM_020510.2, NP_065256.1
• RefSeq Size: 3628
• RefSeq ORF: 1713
• Locus ID: 57265
• MW: 64.5 kDa
• Gene Summary: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. [UniProtKB/Swiss-Prot Function]