Prkci (NM_008857) Mouse Tagged ORF Clone

CAT#: MR209156

Prkci (Myc-DDK-tagged) - Mouse protein kinase C, iota (Prkci)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkci
• Synonyms: 2310021H13Rik; AI427505; aPKClambda; mKIAA4165; Pkci; Pkcl; PKClambda; Prkcl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209156 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCACACGGTCGCGTGCGGCGGCGGCGGGGACCATTCCCACCAGGTCCGGGTGAAAGCCTACTACC
  GCGGGGATATTATGATAACACACTTTGAGCCTTCCATCTCCTTTGAGGGACTTTGCAGTGAGGTTCGAGA
  TATGTGTTCTTTTGACAATGAGCAGCCGTTCACCATGAAATGGATAGATGAGGAAGGAGACCCATGTACA
  GTGTCTTCTCAGTTGGAGTTAGAAGAGGCTTTCAGGCTGTACGAGCTGAACAAGGATTCTGAACTCTTGA
  TTCATGTATTTCCATGTGTACCAGAGCGTCCTGGAATGCCTTGCCCAGGGGAAGACAAGTCCATTTACCG
  GAGAGGGGCACGCCGGTGGAGAAAGCTGTATTGTGCAAATGGCCACACTTTTCAAGCCAAACGTTTCAAT
  AGGCGCGCCCACTGTGCCATCTGCACAGACAGAATCTGGGGCCTCGGACGACAAGGATACAAGTGCATCA
  ACTGCAAACTGCTGGTTCATAAGAAGTGCCACAAGCTGGTCACAATTGAGTGTGGGCGGCACTCTTTGCC
  ACCGGAACCCATGATGCCAATGGACCAGACCATGCATCCAGACCACACACAGACAGTAATTCCATATAAT
  CCTTCAAGTCATGAGAGTTTGGACCAAGTTGGTGAAGAAAAGGAGGCAATGAACACCAGGGAGAGTGGTA
  AAGCGTCGTCCAGTCTAGGTCTGCAGGATTTCGATTTGCTTCGAGTTATAGGGAGGGGAAGTTACGCCAA
  AGTACTGTTGGTTCGGTTAAAGAAAACAGATCGCATTTATGCAATGAAAGTTGTGAAGAAAGAGCTCGTC
  AATGACGATGAGGATATCGATTGGGTGCAGACAGAGAAGCATGTGTTTGAGCAGGCGTCCAATCACCCTT
  TTCTTGTCGGGCTGCATTCTTGCTTCCAGACAGAAAGCAGGTTGTTTTTTGTCATAGAATATGTAAATGG
  AGGGGACCTCATGTTTCATATGCAGCGACAGAGAAAACTTCCTGAAGAGCATGCCAGGTTTTACTCTGCA
  GAAATCAGTCTAGCACTGAATTATCTTCATGAGCGAGGGATAATTTATAGAGATTTGAAGTTGGACAATG
  TACTGCTAGACTCTGAAGGACACATTAAACTCACTGACTACGGCATGTGTAAGGAAGGATTGCGGCCTGG
  AGACACAACCAGCACTTTCTGCGGCACTCCCAATTACATTGCTCCAGAGATCTTAAGAGGAGAAGATTAT
  GGCTTCAGCGTTGACTGGTGGGCTCTTGGAGTGCTTATGTTTGAGATGATGGCGGGAAGGTCTCCGTTTG
  ATATCGTTGGGAGCTCTGACAATCCTGACCAAAACACAGAGGATTATCTATTCCAAGTCATTTTGGAAAA
  GCAGATCCGCATCCCGCGTTCTCTGTCTGTAAAAGCAGCAAGTGTACTGAAGAGTTTTCTCAACAAGGAC
  CCAAAGGAACGATTGGGTTGTCACCCTCAAACTGGATTTGCTGACATTCAAGGACATCCATTCTTCAGAA
  ATGTGGACTGGGACATGATGGAGCAAAAGCAGGTGGTTCCACCCTTTAAACCAAACATTTCTGGAGAATT
  TGGTTTGGATAATTTCGATTCTCAGTTTACTAATGAACCAGTCCAGCTCACTCCAGATGATGATGACATT
  GTGAGGAAGATTGATCAGTCTGAATTTGAAGGTTTTGAGTATATCAACCCCCTCTTGATGTCTGCAGAAG
  AGTGTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209156 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSHTVACGGGGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCSEVRDMCSFDNEQPFTMKWIDEEGDPCT
  VSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFN
  RRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPPEPMMPMDQTMHPDHTQTVIPYN
  PSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELV
  NDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSA
  EISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDY
  GFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLNKD
  PKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDI
  VRKIDQSEFEGFEYINPLLMSAEECV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008857
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008857.1, NM_008857.2
• RefSeq Size: 4465 bp
• RefSeq ORF: 1788 bp
• Locus ID: 18759
• MW: 67.2 kDa