C8a (NM_146148) Mouse Tagged ORF Clone

CAT#: MR209163

C8a (Myc-DDK-tagged) - Mouse complement component 8, alpha polypeptide (C8a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C8a
• Synonyms: MGC29381
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209163 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGTGGTTGCTTTCTTTGGCTTGTCTTTGGTGGCATGGCACCCTGGGGTAACTGCACAAGAGAAGG
  TGAACCAGCGGGTCACCCGAGCTGTGACCCCGCAAGCAGTGTCCTGTCAGTTGAGTGACTGGTACAAATG
  GACAGATTGTTTTCCATGTCAGGATAAAAAGTACCGATACCGGAGCCTCTTGCAGCCAAGCAAGTTTGGA
  GGAACCATCTGCAGTGGTGATATCTGGGATGAGGCCAGCTGTGACAGCCCCACACCTTGTCTAAGGCAAG
  CACAGTGTGGACAGGATTTCCAGTGTAGAGAGACAGGTCGCTGCCTGAAACGCCACCTGGTGTGTAACGG
  AGATAACGACTGCCTGGATGGCTCTGATGAGTCCGACTGTGAAGATGTCAGGGTCACTGAGGATGATTGC
  CATCAGTATGAACCAATCCCAGGATCAGAAAGGGCAGCCTTGGGGTACAACATCCTGACTCAAGAGGAAG
  CTCAGAGTGTTTACGATGCTAAGTATTATGGGGGCCAGTGTGAAACTGTGTACAATGGAGACTGGAGAAA
  GCTGCGGTACGACCCCACCTGTGAGCGCCTCTACTATGGGGAGGATGAGAAATACTTTCGGAAGCCCTAC
  AACTTCCTTAAGTACCACTTTGAAGCCCTGGCGGATACTTCAATCTCCTCAGAGTTTTATGATGATGCGA
  ATGATCTTTTTTTTCACATAAAGAATGGCAAGTCCCACTCAGCTGGTGTAACCGTTGGCGTAGCCCCAGT
  AAAAAGTCCTGTCTCAATAGAGGTGACTGGATCCGGGTCCAAAGCATCTTCATTCCTGAACAAGTTAAAC
  AAGTATAATGAGAAGAGATATGGCTTCATGAGAGTGTCTACAAAGATACAGACTGCACAGTTTAAGATGA
  GAAGGAACAATATTGTGCTGGATGAAGGAATGCTGCAGTCATTAATGGAGCTCCCAGAGCAGTTCAACTA
  TGGCATGTACGCCAAGTTCATCAATGACTATGGCACCCACTACATCACCTCTGGAACCATGGGTGGCATT
  TATGAATATGTTATGGTGCTTGACAAAGAGAAAATGAAAACTGAAGGTACTACTGTGGATGAAGTCCAAA
  AATGCATTGGCGGTGGCATTGGCATAGGAATTAAGGATAGTACAATTGAAGGAGTAGGTATATCAGGAGA
  GTTTTGTGAAAACTCTGGAGATGGTGACCGTGACATAAGGAAGAAAATCACAGGAGTGGAGGACATCATT
  TCTCGGGTGCAAGGTGGAAGTTCTGTCTGGGGCAGTGTCTTGACACACAACAGCAGTGCCATTACATATC
  AGTCCTGGGGGAGATCGTTAAAGTATAACCCTGTTGTTATCGATTTTGAGATGCAGCCTATCTACCAGCT
  ACTGCGGCACACAAACTTGGGACCCCTGGAGACGAAACGTCAGAACCTGCGACGGGCTTTGGACCAGTAC
  CTGATGGAATTTAATGCCTGTCGTTGTGGGCCCTGCTTCAACAATGGGGAACCCATACTTGACGGCACCA
  ACTGCCGGTGTCAGTGTAGTATGGGCCGGCAGGGCCTGGCCTGTGAACGCACGGTGATAGAGGGGCTCAA
  AGACTTCAAAGCCGCTGGCCACTGGAGCTGCTGGAGTTCCTGGTCAGAGTGCAGAGGAGGTTCCCAGGAG
  AGGAGGAGACAGTGCAACAACCCACCACCCAAGAATGGAGGTACCCCATGCCTAGGGAGGAACCTGCAGA
  CCCAGGCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209163 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFVVAFFGLSLVAWHPGVTAQEKVNQRVTRAVTPQAVSCQLSDWYKWTDCFPCQDKKYRYRSLLQPSKFG
  GTICSGDIWDEASCDSPTPCLRQAQCGQDFQCRETGRCLKRHLVCNGDNDCLDGSDESDCEDVRVTEDDC
  HQYEPIPGSERAALGYNILTQEEAQSVYDAKYYGGQCETVYNGDWRKLRYDPTCERLYYGEDEKYFRKPY
  NFLKYHFEALADTSISSEFYDDANDLFFHIKNGKSHSAGVTVGVAPVKSPVSIEVTGSGSKASSFLNKLN
  KYNEKRYGFMRVSTKIQTAQFKMRRNNIVLDEGMLQSLMELPEQFNYGMYAKFINDYGTHYITSGTMGGI
  YEYVMVLDKEKMKTEGTTVDEVQKCIGGGIGIGIKDSTIEGVGISGEFCENSGDGDRDIRKKITGVEDII
  SRVQGGSSVWGSVLTHNSSAITYQSWGRSLKYNPVVIDFEMQPIYQLLRHTNLGPLETKRQNLRRALDQY
  LMEFNACRCGPCFNNGEPILDGTNCRCQCSMGRQGLACERTVIEGLKDFKAAGHWSCWSSWSECRGGSQE
  RRRQCNNPPPKNGGTPCLGRNLQTQAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146148
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_146148.1, NM_146148.2, NP_666260.1
• RefSeq Size: 3636
• RefSeq ORF: 1764
• Locus ID: 230558
• MW: 66.1 kDa
• Gene Summary: This gene encodes the alpha subunit of complement component C8 that participates in the assembly of the complement membrane attack complex. The encoded preproprotein undergoes proteolytic processing to generate the alpha subunit, which associates with the beta and gamma subunits to form a trimeric complement component, C8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. This gene is located adjacent to the gene encoding the beta subunit. [provided by RefSeq, Oct 2015]