Plekha4 (BC021387) Mouse Tagged ORF Clone

CAT#: MR209172

Plekha4 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 (cDNA clone

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plekha4
• Synonyms: PEPP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209172 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGGGCCGACCCCGGAGCAGCCTCAGCCTGGCCAGCAGTGCTTCCACCGTCTCCTCTCTGAGTA
  GCCTGAGCACCAAGAAGCCTACCCGTGCAGTACACAAGGTCCATGCCTTTGGAAAAAGGAACAATGCACT
  TCGACGAGACCCCAACTTGCCGGTGCACATCCGTGGCTGGCTGCACAAACAGGACAGCTCAGGCCTCCGG
  CTGTGGAAGCGCCGTTGGTTCGTCCTTTCAGGCCATTGCCTCTTCTACTATAAGGATAGCAGAGAAGAGA
  GTGTCCTGGGCAGTGTATTATTACCCAGCTACAGTGTCAGGCCAGATGGGCCCGGAGCCCCTCGTGGCCG
  GCGATTCACCTTCACAGCAGAGCACCCGGGCATGAGGACCTATGTCCTTGCAGCTGACACATTAGAGGAC
  CTGAGAGGTTGGCTTCGAGCACTGGGGAGGGCCTCTCGTGCGGAGGGGGAAGATTGCGGACTCCCGAGGT
  CACCTGCACGTCCCAGGCCTGGGGAAGGTCCTGGGGGACCCGGAGGACCCCCAGAGGTGAACAGAAGGGA
  AGAGGGGCGCATCTCAGAATCACCAGAGGTAGCTCGGCTCTCCAGAGGTCTTGGCAGACATGAAACGCAC
  ACTCCCAACTCTACAGTGGATCTGCAGACTGACACCTGGAGTCGTAGAACCCGGAGCCCAGAGTTGTTCT
  CACCCCTTTCCCGGCCTCCCTCCCCTCTGAGCCTTCCCCGTCCTCGCTCTGCCCCGGCCCGGCGACCCCC
  TCTCTCAGCAGGAGACATCTCATTTCCTGCCCGACCGCATACTCCTTTGAGTCGCATCGATGTCCGACCT
  CCTCTGGATTGGGGACCCCAAAGACAGACCCTCTCCCGACCTCCCATTCCACGCCGAGGACCTTCCTCTG
  AAGCTGGCGGAGAACGGCCACCCAGGAGTCCCCAGCCCCGGACACCAGAGCAGAGAACGCAGTCTACCCA
  GGCCACCTCTGGTTCGTCCACCTATCTCCAGCTGCCCCCAAGACCTCCTGGGACCCAGGCCTCCATGATT
  TTGCTGCCAGGACCCCCTGTGGACTCAGCTTTGCATCAGAGCTTGGAGACAGATACGTTGTTGACTAAGC
  TGTGTGGGCAGGACCGGCTCCTGAGGCAGCTACAGGAGGATCTGGACAAGAGGCAGGAGGAGAAGGAGCA
  GCTGGAGGCAGCTTTGGAGCTGACCCGGCAGCAGCTGGGCCAAGCTACCAGGGAGGCTGCAGCTTCCGGG
  AAGGCCTGGGGTCGCCAGCGCCTTCTGCAGGACCGGCTGGTGAACGTGAGGGCTGCTCTGTGTCACCTGG
  CTCAGGAGCGAGAGCGAGTTTGGGACACATACAGCGGCCTGGAGCAGGACCTCGGCACCCTAAGAGAGAC
  GCTCGAGTACCTGCTGCACCTCGGGTCTCCCCAGGACCGTGCGTGTGCTCAACAGCACCTGTGGATGGTG
  GAGGACACGCTGGCAGGTCTGGGTGGCCCCCAGAAACAGCCCCCACATACTGATCCCAAGTCTCCATCCC
  CTGCACCCCAAGGAGAAGAGTCCTCAGAGAGGGAGAGCCTTTCAGAGTCACTGGAGCTCAGCTCCCCTCA
  GTCCCCTGAGGTGGACTGGAGTCGTCCCTCAGGCGGTGACAGAGCCCTCAGCAGCCCTCAGTCAGGTGTT
  GGGTCTCCAAGGGTCTCCCGGGCATCCAGCCCTGAATGTCGTCAACCCTCATCTCCACTGCTGAGAACTA
  AGGTAGACCATCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209172 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEQGRPRSSLSLASSASTVSSLSSLSTKKPTRAVHKVHAFGKRNNALRRDPNLPVHIRGWLHKQDSSGLR
  LWKRRWFVLSGHCLFYYKDSREESVLGSVLLPSYSVRPDGPGAPRGRRFTFTAEHPGMRTYVLAADTLED
  LRGWLRALGRASRAEGEDCGLPRSPARPRPGEGPGGPGGPPEVNRREEGRISESPEVARLSRGLGRHETH
  TPNSTVDLQTDTWSRRTRSPELFSPLSRPPSPLSLPRPRSAPARRPPLSAGDISFPARPHTPLSRIDVRP
  PLDWGPQRQTLSRPPIPRRGPSSEAGGERPPRSPQPRTPEQRTQSTQATSGSSTYLQLPPRPPGTQASMI
  LLPGPPVDSALHQSLETDTLLTKLCGQDRLLRQLQEDLDKRQEEKEQLEAALELTRQQLGQATREAAASG
  KAWGRQRLLQDRLVNVRAALCHLAQERERVWDTYSGLEQDLGTLRETLEYLLHLGSPQDRACAQQHLWMV
  EDTLAGLGGPQKQPPHTDPKSPSPAPQGEESSERESLSESLELSSPQSPEVDWSRPSGGDRALSSPQSGV
  GSPRVSRASSPECRQPSSPLLRTKVDHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC021387
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021387, AAH21387
• RefSeq Size: 2158 bp
• RefSeq ORF: 1766 bp
• Locus ID: 69217
• MW: 64.8 kDa