Cdk5rap1 (NM_025876) Mouse Tagged ORF Clone

CAT#: MR209175

Cdk5rap1 (Myc-DDK-tagged) - Mouse CDK5 regulatory subunit associated protein 1 (Cdk5rap1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdk5rap1
• Synonyms: 2310066P17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209175 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCTTTACGGTGTGTCCTCCAAGTACAGAGGTTGTCAGCACCATTCACCTCCATGTGCTGGGTGT
  TGCTTAGGACCTGCAGAGCACAAAGCAGTGTGTCCAGCACTCCTTGTCCCAGTCCAGAGGCGAAGAGCTC
  AGAAGCTCAGAAGGACTTCAGCTCCAGGCTAGCCACTGGACCGACTTTTCAGCATTTTTTAAGAAGTGCT
  TCAGTTCCTCAAGAGAAACCATCTTCTCCAGAGGTGGAGGACCCACCTCCCTATCTCTCGGGGGATGAAC
  TTCTAGGAAGGCAGAGAAAAGTCTACCTCGAGACCTATGGCTGTCAGATGAACGTGAACGACACAGAGAT
  AGCCTGGTCCATCTTACAGAAGAGTGGCTACCTTCGGACCAGCAACCTCCAAGAGGCTGATGTGATCCTT
  CTTGTCACGTGTTCTATCAGGGAGAAGGCCGAGCAGACCATCTGGAACCGCTTACATCAGCTCAAAGTCC
  TGAAGACAAAGCGGCCACGCTCTCGAGTACCTCTGAGGATTGGGATTCTAGGCTGCATGGCTGAGAGGCT
  GAAGGGAGAGATCCTCAACAGGGAGAAGATGGTAGATCTCTTGGCTGGTCCAGACGCCTACCGAGACCTT
  CCCCGGCTGCTGGCTGTGGTGGAGTCAGGTCAGCAAGCAGCAAATGTGCTTCTCTCTCTGGATGAGACCT
  ACGCAGATATCATGCCAGTCCAGACGAGCCCCAGTGCCACTTCTGCCTTTGTATCCATTATGCGGGGCTG
  CGACAACATGTGCAGTTACTGTATCGTGCCGTTCACGCGTGGCCGGGAGAGGAGCCGGCCAGTTGCCTCC
  ATTCTCGATGAAGTGAGGAAGCTGTCCGAGCAGGGCCTAAAAGAAGTGACACTTCTTGGTCAGAATGTTA
  ATAGTTTTCGGGACAATTCGGAAGTCCAGTTCAATAATGCAGGGTCTGCTAACCTCAGCCGTGGCTTTAC
  CACCAACTATAAACCCAAACAAGGAGGGCTTCGTTTTTCTCACCTTCTGGATCAGGTTTCAAGAATCGAT
  CCTGAAATGAGGATCCGATTCACCTCTCCTCACCCCAAGGATTTTCCTGATGAGGTTCTTCAGCTGATTC
  GTGAGAGACACAACATCTGTAAGCAGATCCACCTGCCAGCCCAGAGTGGAAGCAGCCGTGTACTGGATGC
  CATGCGGAGAGGATATTCAAGAGAAGCATATGTGGCATTAGTTCATCATGTCAGAGAGACTATCCCAGGT
  GTGAGCCTTAGCAGCGACTTCATCACTGGCTTTTGTGGAGAGACAGAGGATGATCACCGGCAGACAGTGT
  CTTTACTTAGAGAAGTGCAGTATAATACCGGTTTTCTCTTTGCATACAGCATGAGGCAAAAGACACGAGC
  ATATCACAGGCTGAAGGATGATGTTCCAGAAGAAGTAAAATTAAGGCGTTTGGAGGAACTCATTACTGTC
  TTCAGAGAAGAAGCTTCAAAAGCCAATAAGACCTCAGTGGGTTGTAGCCAGCTGGTACTGGTTGAAGGGT
  TCAGCAAACGTTCTACCACAGACCTTTGTGGCCGGAATGATGCAAACCTTAAGGTGATTTTCCCTGACGC
  AGAGGTGGAGGATATCACTAACCCTGGGCTCAAGGTCAGAGCTCAACCTGGGGACTATGTGCTGGTGAAG
  ATCACCTCTGCCAGCTCTCAGACCCTGAAAGGACACATTCTCTGCAGGACCACTATGAAAGACTCATTGA
  CGTATTGCACGACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209175 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHPLRCVLQVQRLSAPFTSMCWVLLRTCRAQSSVSSTPCPSPEAKSSEAQKDFSSRLATGPTFQHFLRSA
  SVPQEKPSSPEVEDPPPYLSGDELLGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVIL
  LVTCSIREKAEQTIWNRLHQLKVLKTKRPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDL
  PRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPVAS
  ILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFNNAGSANLSRGFTTNYKPKQGGLRFSHLLDQVSRID
  PEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQSGSSRVLDAMRRGYSREAYVALVHHVRETIPG
  VSLSSDFITGFCGETEDDHRQTVSLLREVQYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITV
  FREEASKANKTSVGCSQLVLVEGFSKRSTTDLCGRNDANLKVIFPDAEVEDITNPGLKVRAQPGDYVLVK
  ITSASSQTLKGHILCRTTMKDSLTYCTT
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025876
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025876.1, NM_025876.2, NP_080152.1
• RefSeq Size: 1970 bp
• RefSeq ORF: 1767 bp
• Locus ID: 66971
• MW: 66.1 kDa