Hdac9 (NM_024124) Mouse Tagged ORF Clone

CAT#: MR209176

Hdac9 (Myc-DDK-tagged) - Mouse histone deacetylase 9 (Hdac9)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hdac9
• Synonyms: AV022454; D030072B18Rik; HD7B; HD9; Hdac7b; HDRP; Mitr; mKIAA0744
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209176 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAGTATGATCAGCTCAGTGGACGTGAAATCAGAGGTTCCTATGGGCCTGGAGCCCATCTCACCTT
  TAGACCTGAGGACAGATCTCAGGATGATGATGCCTGTGGTGGATCCTGTCGTCCGCGAGAAGCAACTGCA
  GCAAGAGTTACTTCTTATTCAGCAGCAGCAGCAGATCCAGAAGCAGCTCCTCATAGCAGAGTTTCAGAAG
  CAGCACGAGAATTTGACAAGGCAGCATCAAGCACAACTCCAGGAGCACATCAAGGAACTTCTAGCCATAA
  AACAACAGCAAGAACTCCTAGAAAAGGAGCAGAAACTGGAGCAGCAGAGGCAAGAACAGGAAGTAGAGAG
  GCATCGCAGAGAGCAGCAGCTTCCTCCTCTCAGAGGCAAAGATAGAGGACGAGAAAGGGCAGTGGCAAGC
  ACAGAGGTTAAGCAGAAGCTTCAAGAATTCCTGTTGAGTAAATCAGCAACAAAAGACACTCCAACCAATG
  GAAAGAATCATTCCGTGGGCCGCCATCCCAAGCTCTGGTACACGGCTGCCCACCACACATCACTGGATCA
  AAGCTCTCCACCCCTCAGTGGAACGTCTCCATCCTACAAGTACACATTACCAGGAGCTCAAGATAGCAAG
  GATGATTTCCCCTTGAGAAAAACTGCCTCAGAGCCCAACTTGAAGGTGCGGTCCAGGTTAAAACAGAAAG
  TGGCAGAGAGGAGAAGCAGTCCCTTACTCAGGCGGAAGGATGGAAATCTTGTCACTTCATTCAAGAAGCG
  AGTGTTTGAGGTGGCAGAATCCTCGGTCAGTAGCAGCTCTCCAGGGTCAGGTCCCAGTTCACCAAACAAT
  GGCCCTGCTGGGAATGTGACCGAAAATGAGGCTTCAGCTCTGCCTCCCACGCCTCACCCCGAGCAACTGG
  TTCCACAGCAGCGCATACTAATTCATGAAGATTCCATGAACCTGCTAAGTCTCTATACCTCCCCGTCCCT
  GCCCAATATCACTCTGGGACTTCCAGCAGTGCCGTCCCCACTCAATGCTTCTAACTCACTCAAAGACAAA
  CAGAAGTGCGAGACACAGATGCTCAGACAAGGTGTTCCTCTGCCCAGTCAGTATGGCAGTAGCATTGCAG
  CGTCCTCCAGCCACGTTCATGTAGCAATGGAAGGAAAACCCACCAGCAGCCACCAGGCTCTCCTGCAGCA
  CCTACTGCTGAAGGAACAAATGCGACAGCAAAAGCTTCTCGTGGCTGGTGGAGTTCCATTACACCCTCAG
  TCTCCTTTGGCAACAAAAGAAAGAATTTCACCAGGCATTAGAGGTACCCACAAATTGCCCCGCCACCGAC
  CCCTGAATCGAACCCAGTCTGCACCTTTGCCTCAGAGCACGCTGGCTCAGCTGGTCATTCAGCAGCAGCA
  CCAGCAGTTCCTGGAGAAGCAGAAACAATACCAGCAGCAGATCCACATGAACAAACTGCTATCGAAATCT
  ATTGAACAACTGAAGCAACCAGGCAGCCACCTTGAAGAAGCAGAGGAGGAGCTTCAGGGGGACCAATCCA
  TGGAAGACAGAGCAGCCTCTAAGGACAACAGTGCTAGGAGTGACAGCAGTGCTTGTGTGGAGGACACGCT
  GGGACAAGTTGGGGCTGTGAAGGTCAAGGAGGAACCCGTGGACAGCGATGAAGATGCTCAGATACAGGAA
  ATGGAATGTGGGGAGCAGGCTGCTTTTATGCAACAGGTAATAGGCAAAGATTTAGCTCCAGGATTTGTAA
  TTAAAGTCATTATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209176 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHSMISSVDVKSEVPMGLEPISPLDLRTDLRMMMPVVDPVVREKQLQQELLLIQQQQQIQKQLLIAEFQK
  QHENLTRQHQAQLQEHIKELLAIKQQQELLEKEQKLEQQRQEQEVERHRREQQLPPLRGKDRGRERAVAS
  TEVKQKLQEFLLSKSATKDTPTNGKNHSVGRHPKLWYTAAHHTSLDQSSPPLSGTSPSYKYTLPGAQDSK
  DDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGNLVTSFKKRVFEVAESSVSSSSPGSGPSSPNN
  GPAGNVTENEASALPPTPHPEQLVPQQRILIHEDSMNLLSLYTSPSLPNITLGLPAVPSPLNASNSLKDK
  QKCETQMLRQGVPLPSQYGSSIAASSSHVHVAMEGKPTSSHQALLQHLLLKEQMRQQKLLVAGGVPLHPQ
  SPLATKERISPGIRGTHKLPRHRPLNRTQSAPLPQSTLAQLVIQQQHQQFLEKQKQYQQQIHMNKLLSKS
  IEQLKQPGSHLEEAEEELQGDQSMEDRAASKDNSARSDSSACVEDTLGQVGAVKVKEEPVDSDEDAQIQE
  MECGEQAAFMQQVIGKDLAPGFVIKVII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024124
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024124.1, NM_024124.2, NM_024124.3, NP_077038.2
• RefSeq Size: 4461 bp
• RefSeq ORF: 1767 bp
• Locus ID: 79221
• MW: 65.7 kDa