Zwilch (BC027435) Mouse Tagged ORF Clone

CAT#: MR209182

Zwilch (Myc-DDK-tagged) - Mouse Zwilch, kinetochore associated, homolog (Drosophila) (cDNA clone MGC:35649 IMAGE:2812472)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zwilch
• Synonyms: 2310031L18Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209182 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTCGCGAATGAACCGCGCGGCGGAGGAGTTTTATGCTCGTCTCCGGCAGGAATTTAATGAAGAGA
  AGAAAGGAGCCTCTAAAGACCCATTCATCTATGAGGCTGATGTACAAGTGCAGTTGATCAGCAAAGGCCA
  ACCAAGTCTTCTGAAAACTATTCTAAATGAAAATGACTCAGTGTTTCTCGTGGAAAAAGTGGTTTTGGAG
  AAGGAAGAAACAAGTCAAGTTGAAGAACTGCAGTCTGAAGAAACTGCTATTTCTGATCTCTCTGCTGGTG
  AAAATATTAGGCCACTGGCCTTACCTGTTGGGAGAGCAAGGCAGTTAATTGGGCTCTATACCATGGCTCA
  CAATCCTAATATGACTCATCTGAAGATTAAGCAGCCAGTGACAGCCCTTCCTCCTCTTTGGGTGAGATGT
  GACGGTTCAGATCCTGAAGGTACCTGTTGGCTAGGAGCTGAGCTTATCACAACAAATGACATCATTGCAG
  GCGTTATCCTGTATGTGCTCACTTGTAAAGCTGATAAAAATTACTCTGAAGATCTGGAAAATTTAAAAAC
  TTCACACAAGAAAAGACATCACGTGTCTGCTGTGACAGCCAGAGGCTTTGCCCAGTATGAGCTCTTTAAG
  TCTGACGACTTGGATGACACTGTTGCCCCATCACAAACTACAGTCACGCTGGACCTTTCCTGGAGCCCTG
  TGGATGAGATGCTCCAAACCCCTCCCCTGTCTTCAACTGCAGCTCTGAATATCAGAGTACAATCAGGAGA
  GTCCAGAGGTTGTCTGAGTCATCTTCACAGAGAACTGAAATTTCTCCTTGTTTTAGCTGATGGCATCAGG
  ACTGGTGTAACTGAATGGCTTGAGCCTCTGGAAACAAAATCTGCTCTTGAATTTGTTCAGGAATTTCTGA
  ATGACTTAAATAAACTGGATGAATTTGATGATTCTACAAAAAAAGATAAACAAAAAGAGGCAGTGAACCA
  TGACGCAGCTGCAGTTGTCAGGTCCATGCTTCTCACCGTGCGGGGGGATCTCGATTTTGCTGAACAGCTT
  TGGTGTAGAATGAGCAGCAGTGTGGTTTCCTATCAAGACTTGGTGAAGTGTTTCACACTCATCCTGCAGA
  GTCTTCAGCGTGGTGATATACAGCCATGGTTGCACAGTGGAAGTAACAGTTTACTAAGTAAACTCATTCA
  TCAGTCTTACCATGGAGCTATGGACAGTGTCCCTCTCTCTGGAACTACCCCACTGCAGATGCTTTTGGAA
  ATTGGTTTGGACAAACTGAAGAAAGATTATATCAGCTTTTTTGTCAGTCAGGAACTTGCATCTTTGAATC
  ATTTGGAATACTTCATTTCTCCCTCAGTATCGACACAGGAACAGGTTTGCCGTGTTCAGAAACTCCATCA
  TATTCTTGAAATCTTAGTCATCTGCATGCTTTTCATTAAACCTCAGCATGAGCTTCTCTTCTCTTTAACA
  CAATCCTGCATAAAATATTATAAACAAAATCCTCTTGATGAGCAACACATTTTTCAGTTGCCAGTCAGAC
  CAGCTGCTGTCAAGAATTTGTATCAAAGTGAGAAGCCGCAGAAATGGAGAGTGGAGCTCAGCAATAGTCA
  GAAGAGGGTGAAAACAGTGTGGCAACTGAGTGACAGCTCCCCTGTAGACCATTCCAGTTTCCACAGGCCT
  GAGTTTCCTGAGCTGACACTGAATGGTAGTCTGGAAGAAAGGACAGCCTTCGTTAACATGCTTACCTGCA
  GCCAGGTGCACTTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209182 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWSRMNRAAEEFYARLRQEFNEEKKGASKDPFIYEADVQVQLISKGQPSLLKTILNENDSVFLVEKVVLE
  KEETSQVEELQSEETAISDLSAGENIRPLALPVGRARQLIGLYTMAHNPNMTHLKIKQPVTALPPLWVRC
  DGSDPEGTCWLGAELITTNDIIAGVILYVLTCKADKNYSEDLENLKTSHKKRHHVSAVTARGFAQYELFK
  SDDLDDTVAPSQTTVTLDLSWSPVDEMLQTPPLSSTAALNIRVQSGESRGCLSHLHRELKFLLVLADGIR
  TGVTEWLEPLETKSALEFVQEFLNDLNKLDEFDDSTKKDKQKEAVNHDAAAVVRSMLLTVRGDLDFAEQL
  WCRMSSSVVSYQDLVKCFTLILQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGAMDSVPLSGTTPLQMLLE
  IGLDKLKKDYISFFVSQELASLNHLEYFISPSVSTQEQVCRVQKLHHILEILVICMLFIKPQHELLFSLT
  QSCIKYYKQNPLDEQHIFQLPVRPAAVKNLYQSEKPQKWRVELSNSQKRVKTVWQLSDSSPVDHSSFHRP
  EFPELTLNGSLEERTAFVNMLTCSQVHFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027435
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027435, AAH27435
• RefSeq Size: 2745 bp
• RefSeq ORF: 1769 bp
• Locus ID: 68014
• MW: 66.8 kDa