Cry2 (BC054794) Mouse Tagged ORF Clone

CAT#: MR209229

Cry2 (Myc-DDK-tagged) - Mouse cryptochrome 2 (photolyase-like) (cDNA clone MGC:65642 IMAGE:6834104)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cry2
• Synonyms: AV006279|D130054K12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209229 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCTGCTGTGGTGGCAGCGACGGTCCCCGCGCAATCGATGGGCGCGGACGGCGCGTCCTCGG
  TGCACTGGTTCCGCAAAGGACTACGGCTCCACGACAACCCCGCGCTGCTAGCTGCCGTGCGCGGGGCGCG
  CTGTGTGCGCTGCGTCTACATCCTCGACCCGTGGTTCGCGGCCTCCTCGTCTGTGGGCATCAACCGATGG
  AGGTTCCTACTGCAATCTCTGGAAGATCTGGACACAAGCTTAAGAAAGCTGAATTCGCGTCTGTTTGTAG
  TCCGGGGACAGCCAGCTGATGTGTTCCCAAGGCTGTTCAAGGAATGGGGGGTGACCCGTTTGACCTTTGA
  ATATGACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATTATGAAGATGGCCAAGGAGGCTGGCGTG
  GAGGTGGTGACTGAGAACTCTCACACCCTCTATGACCTAGACAGAATCATCGAACTGAATGGGCAGAAAC
  CACCCCTTACCTACAAGCGCTTTCAGGCCCTCATCAGCCGCATGGAGCTGCCCAAGAAGCCCGCGGTGGC
  TGTGAGCAGCCAGCAGATGGAGAGCTGCAGAGCTGAGATCCAGGAGAACCATGACGACACCTATGGCGTG
  CCTTCCCTGGAGGAGCTGGGATTCCCCACGGAAGGACTTGGCCCAGCTGTTTGGCAAGGAGGAGAGACAG
  AAGCTCTGGCCCGCCTGGACAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCTCGGAT
  GAATGCCAATTCCTTACTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGATGCCTCTCCTGC
  CGCCTCTTCTACTACCGCCTGTGGGACTTGTACAAGAAGGTGAAGAGGAACAGCACACCCCCCCTCTCCT
  TATTTGGACAACTCCTGTGGCGAGAATTCTTCTACACAGCGGCCACCAACAACCCCAGGTTTGACCGAAT
  GGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAACCCCGAAGCCCTGGCCAAGTGGGCCGAG
  GGCAAGACAGGCTTCCCTTGGATTGACGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACC
  TGGCCCGGCACGCTGTGGCCTGCTTCCTCACCCGCGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCG
  GGTATTTGACGAGCTGCTCCTGGATGCCGATTTCAGTGTGAATGCAGGCAGCTGGATGTGGCTGTCCTGC
  AGTGCTTTCTTCCAACAATTCTTCCACTGCTACTGCCCTGTGGGCTTCGGCCGACGTACAGACCCCAGTG
  GGGACTACATCCGGCGGTACCTGCCCAAACTGAAAGGCTTCCCCTCTCGATACATCTATGAGCCCTGGAA
  TGCCCCCGAGTCAGTTCAGAAGGCTGCCAAGTGCATCATTGGCGTGGACTACCCACGGCCCATCGTCAAT
  CATGCAGAGACTAGTCGGCTCAACATTGAACGAATGAAGCAGATCTACCAACAGCTGTCGAGATACCGGG
  GACTCTGTCTATTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCACCCTGTGGCAGAGCCTGGTTC
  AAGCCAAGCTGGGAGCATCAGCAACACAGGCCCCAGAGCACTATCCAGTGGCCCAGCTTCCCCCAAACGC
  AAGCTGGAAGCAGCCGAGGAACCTCCTGGTGAAGAACTGACCAAGCGGGCTAGAGTGACGGAGATGCCTA
  CCCAAGAGCCAGCAAGCAAGGACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209229 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAAVVAATVPAQSMGADGASSVHWFRKGLRLHDNPALLAAVRGARCVRCVYILDPWFAASSSVGINRW
  RFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAGV
  EVVTENSHTLYDLDRIIELNGQKPPLTYKRFQALISRMELPKKPAVAVSSQQMESCRAEIQENHDDTYGV
  PSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFGCLSC
  RLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWAE
  GKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSVNAGSWMWLSC
  SAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKGFPSRYIYEPWNAPESVQKAAKCIIGVDYPRPIVN
  HAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPGSSQAGSISNTGPRALSSGPASPKR
  KLEAAEEPPGEELTKRARVTEMPTQEPASKDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC054794
• ORF Size: 1776 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC054794, AAH54794
• RefSeq Size: 3975 bp
• RefSeq ORF: 1778 bp
• Locus ID: 12953
• MW: 66.9 kDa
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]