Sh3bp1 (BC004598) Mouse Tagged ORF Clone

CAT#: MR209303

Sh3bp1 (Myc-DDK-tagged) - Mouse SH3-domain binding protein 1 (cDNA clone MGC:6037 IMAGE:3584958)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sh3bp1
• Synonyms: 3BP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209303 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGAGCTTCAAGGAACTGGATCCTGATTCCAGCATGGGGAAGGCCTTGGAGATGACCTGCGCCA
  TCCAGAACCAGCTGGCCCGAATCCTAGCCGAGTTTGAGATGACCCTGGAGAGGGATGTCCTGCAGCCGCT
  CAGCAGGCTGAGCGAGGAGGAGCTGCCTGCCATCCTGAAGCACAAGAAAAGCCTTCAGAAGCTGGTGTCT
  GACTGGAACACCCTCAAAAGCAGGCTCAGCCAGGCAGCCAAGAACTCAGGAAGCAACCAAGGCCTGGGTG
  GTGCCTCAGGCAGTCACACCCACACCACCACTGCCAACAAGGTAGAGATGCTGAAGGAGGAAGAGGAGGA
  GCTCAAGAAGAAGGTGGAACAGTGCAAGGACGAGTACCTGGCCGATCTCTACCACTTCTCCACCAAGGAA
  GATTCTTATGCCAACTACTTTATTCATCTCTTGGAGATTCAGGCTGACTACCACCGCAAGTCACTGACCT
  CACTCGACACGGCCCTAGCTGAGCTGAGGGACAACCACAACCAAGCAGACCACTCCCCCTTGACGACGGC
  TGCCCCCTTCTCCAGGGTATATGGGGTATCTTTGAGAACCCACCTACAAGACCTAGGCCGTGACATTGCC
  CTGCCCATCGAGGCCTGCGTCCTGTTGCTGCTGTCAGAGGGCATGCAGGAGGAGGGCCTCTTCCGTCTGG
  CTGCTGGGGCCTCTGTGCTGAAGCGCCTTAAGCAGACCATGGCCTCAGATCCCCACAGCCTAGAGGAATT
  CTGCTCAGACCCCCATGCTGTAGCAGGTGCCCTCAAGTCCTATCTCCGGGAGCTGCCGGAGCCCCTGATG
  ACCTCTGACCTCTACGATGACTGGATGAGAGCAGCCAGCCTGAAGGAGCCAGGAGCCCGCCTGGAAGCCC
  TCCATGATGTTTGCAGCCGTCTACCCCAAGAGAACTTCAACAACCTCAGGTACCTGATGAAGTTCCTGGC
  TCTGCTGGCAGAGGAGCAGGATGTAAACAAGATGACACCCAGCAACATCGCCATTGTCCTAGGACCCAAC
  CTGCTGTGGCCTCCCGAGAAAGAAGGGGACCAGGCCCAACTGGACGCTGCCTCTGTGTCCTCCATTCAGG
  TGGTGGGTGTTGTCGAGGCGCTGATACAGAACGCAGACACCCTCTTCCCTGGAGATATCAACTTCAATGT
  ATCAGGCATCTTCCCAGGCCTGGCCCCCCAGGAGAAGGTCAGTAGCCAACAGGTCTCAGAGGAGTTGCCA
  CCTGTTACTGTGCCTGCCCCAGCCACGACCCCAGCTCCCACCCTGGCTCCAGCCTCAATGGCTGTCAGAG
  AAAGGACAGAGGCTGACTTGCCCAAACCAACTTCTCCCAAGGTCAGCAGGAACCCCACCGAAACAGCTGC
  TTCGGCGGAGGACATGACCCGTAAGACCAAGCGCCCAGCCCCAGCCCGGCCTACCATGCCACCTCCCCAG
  CCCTCCAGCACCCGCTCATCCCCTCCAGCTCCATCCCTGCCCCCTGGCTCTGTCAGCCCTGGTACTCCCC
  AAGCTCTGCCTCGCCGTCTGGTGGGCACCAGCCTCCGGGCCCCTACCATGCCTCCCCCGCTACCCCCTGT
  TCCTCCACAGCCTGCCCGGCGTCAGAGCCGGCGTTTACCAGCCTCCCCTGTCATCTCAAATATGCCTGCT
  CAGGTGGACCAGGGAGTAGCTACAGAGGACAGAGAAGGCCCTGAGGCTGTGGGTGGGCATCCCCCTCCAC
  CAGCTCTGCCACCCCAGCCCCGGCCCAGGGGCCTCATTTCAGAGACAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209303 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAESFKELDPDSSMGKALEMTCAIQNQLARILAEFEMTLERDVLQPLSRLSEEELPAILKHKKSLQKLVS
  DWNTLKSRLSQAAKNSGSNQGLGGASGSHTHTTTANKVEMLKEEEEELKKKVEQCKDEYLADLYHFSTKE
  DSYANYFIHLLEIQADYHRKSLTSLDTALAELRDNHNQADHSPLTTAAPFSRVYGVSLRTHLQDLGRDIA
  LPIEACVLLLLSEGMQEEGLFRLAAGASVLKRLKQTMASDPHSLEEFCSDPHAVAGALKSYLRELPEPLM
  TSDLYDDWMRAASLKEPGARLEALHDVCSRLPQENFNNLRYLMKFLALLAEEQDVNKMTPSNIAIVLGPN
  LLWPPEKEGDQAQLDAASVSSIQVVGVVEALIQNADTLFPGDINFNVSGIFPGLAPQEKVSSQQVSEELP
  PVTVPAPATTPAPTLAPASMAVRERTEADLPKPTSPKVSRNPTETAASAEDMTRKTKRPAPARPTMPPPQ
  PSSTRSSPPAPSLPPGSVSPGTPQALPRRLVGTSLRAPTMPPPLPPVPPQPARRQSRRLPASPVISNMPA
  QVDQGVATEDREGPEAVGGHPPPPALPPQPRPRGLISETE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC004598
• ORF Size: 1800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004598, AAH04598
• RefSeq Size: 2224 bp
• RefSeq ORF: 1802 bp
• Locus ID: 20401
• MW: 65.3 kDa