Afp (NM_007423) Mouse Tagged ORF Clone

CAT#: MR209348

Afp (Myc-DDK-tagged) - Mouse alpha fetoprotein (Afp)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Afp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209348 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGGATCACACCCGCTTCCCTCATCCTCCTGCTACATTTCGCTGCGTCCAAAGCATTGCACGAAA
  ATGAGTTTGGGATAGCTTCCACGTTAGATTCCTCCCAGTGCGTGACGGAGAAGAATGTGCTTAGCATAGC
  TACCATCACCTTTACCCAGTTTGTTCCGGAAGCCACCGAGGAGGAAGTGAACAAAATGACTAGCGATGTG
  TTGGCTGCAATGAAGAAAAACTCTGGCGATGGGTGTTTAGAAAGCCAGCTATCTGTGTTTCTGGATGAAA
  TTTGTCATGAGACGGAACTCTCTAACAAGTATGGACTCTCAGGCTGCTGCAGCCAAAGTGGAGTGGAAAG
  ACATCAGTGTCTGCTGGCACGCAAGAAGACTGCTCCGGCCTCTGTCCCACCCTTCCAGTTTCCAGAACCT
  GCCGAGAGTTGCAAAGCACATGAAGAAAACAGGGCAGTGTTCATGAACAGGTTCATCTATGAAGTGTCAA
  GGAGGAACCCCTTCATGTATGCCCCAGCCATTCTGTCCTTGGCTGCTCAGTACGACAAGGTCGTTCTGGC
  ATGCTGCAAAGCTGACAACAAGGAGGAGTGCTTCCAGACAAAGAGAGCATCCATTGCAAAGGAATTAAGA
  GAAGGAAGCATGTTAAATGAGCATGTATGTTCAGTGATAAGAAAATTTGGATCCCGAAACCTCCAGGCAA
  CAACCATTATTAAGCTAAGTCAAAAGTTAACTGAAGCAAATTTTACTGAGATTCAGAAGCTGGCCCTGGA
  TGTGGCTCACATCCACGAGGAGTGTTGCCAAGGAAACTCGCTGGAGTGTCTGCAGGATGGGGAAAAAGTC
  ATGACATATATATGTTCTCAACAAAATATTCTGTCAAGCAAAATAGCAGAGTGCTGCAAATTACCCATGA
  TCCAACTAGGCTTCTGCATAATTCACGCAGAGAATGGCGTCAAACCTGAAGGCTTATCTCTAAATCCAAG
  CCAGTTTTTGGGAGACAGAAATTTTGCCCAATTTTCTTCAGAGGAAAAAATCATGTTCATGGCAAGCTTT
  CTTCATGAATACTCAAGAACTCACCCCAACCTTCCTGTCTCAGTCATTCTAAGAATTGCTAAAACGTACC
  AGGAAATATTGGAGAAGTGTTCCCAGTCTGGAAATCTACCTGGATGTCAGGACAATCTGGAAGAAGAATT
  GCAGAAACACATCGAGGAGAGCCAGGCACTGTCCAAGCAAAGCTGCGCTCTCTACCAGACCTTAGGAGAC
  TACAAATTACAAAATCTGTTCCTTATTGGTTACACGAGGAAAGCCCCTCAGCTGACCTCAGCAGAGCTGA
  TCGACCTCACCGGGAAGATGGTGAGCATTGCCTCCACGTGCTGCCAGCTCAGCGAGGAGAAATGGTCCGG
  CTGTGGTGAGGGAATGGCCGACATTTTCATTGGACATTTGTGTATAAGGAATGAAGCAAGCCCTGTGAAC
  TCTGGTATCAGCCACTGCTGCAACTCTTCGTATTCCAACAGGAGGCTATGCATCACCAGTTTTCTGAGGG
  ATGAAACCTATGCCCCTCCCCCATTCTCTGAGGATAAATTCATCTTCCACAAGGATCTGTGCCAAGCTCA
  GGGCAAAGCCCTACAGACCATGAAACAAGAGCTTCTCATTAACCTGGTGAAGCAAAAGCCTGAACTGACA
  GAGGAGCAGCTGGCGGCTGTCACTGCAGATTTCTCGGGCCTTTTGGAGAAGTGCTGCAAAGCCCAGGACC
  AGGAAGTCTGTTTCACAGAAGAGGGTCCAAAGTTGATTTCCAAAACTCGTGATGCTTTGGGCGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209348 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKWITPASLILLLHFAASKALHENEFGIASTLDSSQCVTEKNVLSIATITFTQFVPEATEEEVNKMTSDV
  LAAMKKNSGDGCLESQLSVFLDEICHETELSNKYGLSGCCSQSGVERHQCLLARKKTAPASVPPFQFPEP
  AESCKAHEENRAVFMNRFIYEVSRRNPFMYAPAILSLAAQYDKVVLACCKADNKEECFQTKRASIAKELR
  EGSMLNEHVCSVIRKFGSRNLQATTIIKLSQKLTEANFTEIQKLALDVAHIHEECCQGNSLECLQDGEKV
  MTYICSQQNILSSKIAECCKLPMIQLGFCIIHAENGVKPEGLSLNPSQFLGDRNFAQFSSEEKIMFMASF
  LHEYSRTHPNLPVSVILRIAKTYQEILEKCSQSGNLPGCQDNLEEELQKHIEESQALSKQSCALYQTLGD
  YKLQNLFLIGYTRKAPQLTSAELIDLTGKMVSIASTCCQLSEEKWSGCGEGMADIFIGHLCIRNEASPVN
  SGISHCCNSSYSNRRLCITSFLRDETYAPPPFSEDKFIFHKDLCQAQGKALQTMKQELLINLVKQKPELT
  EEQLAAVTADFSGLLEKCCKAQDQEVCFTEEGPKLISKTRDALGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007423
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007423.1, NM_007423.2, NM_007423.3, NM_007423.4, NP_031449.2
• RefSeq Size: 2086 bp
• RefSeq ORF: 1818 bp
• Locus ID: 11576
• MW: 67.3 kDa
• Gene Summary: Binds estrogens, fatty acids and metals. [UniProtKB/Swiss-Prot Function]