Tec (NM_001113461) Mouse Tagged ORF Clone

CAT#: MR209373

Tec (Myc-DDK-tagged) - Mouse tec protein tyrosine kinase (Tec), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tec
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209373 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTCAACACTATCCTAGAAGAGATTCTTATTAAAAGGTCCCAGCAGAAAAAGAAGACATCACCCT
  TAAACTACAAAGAGAGACTTTTTGTACTTACAAAATCCGTGTTGAGCTACTATGAGGGTCGAGCGGAGAA
  GAAATACAGAAAGGGCGTCATTGATATTTCCAAAATCAAGTGTGTGGAGATAGTGAAGAACGATGATGGT
  GTCATTCCCTGTCAAAATAAATTTCCATTCCAGGTTGTTCATGATGCTAATACACTTTATATTTTTGCAC
  CTAGTCCACAAAGCAGGGACCGATGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATCAT
  GATTAAATACCATCCTAAATTCTGGGCAGATGGGAGTTACCAGTGTTGTAGACAAACAGAAAAACTAGCA
  CCCGGATGTGAGAAGTACAATCTTTTTGAGAGTAGTATAAGAAAGACCCTGCCTCCCGCGCCAGAAATAA
  AGAAGAGAAGGCCTCCTCCACCAATTCCCCCAGAGGAAGAAAATACTGAAGAAATCGTTGTAGCGATGTA
  TGACTTCCAAGCGACGGAAGCACATGACCTCAGGTTAGAGAGAGGCCAAGAGTATATCATCCTGGAAAAG
  AATGACCTCCATTGGTGGAGAGCGAGAGATAAGTATGGGTGGTACTGCAGAAATACCAACAGAAGCAAAG
  CAGAACAGCTCCTCAGAACGGAAGATAAAGAAGGTGGTTTTATGGTGAGAGACTCCAGTCAACCAGGCTT
  GTACACTGTCTCCCTTTACACAAAGTTTGGGGGAGAAGGCTCATCAGGTTTCAGGCATTATCACATAAAG
  GAAACAGCAACATCCCCAAAGAAGTATTACCTGGCAGAGAAGCATGCTTTCGGGTCCATTCCTGAGATCA
  TTGAATATCACAAGCACAATGCGGCAGGGCTTGTCACCAGGCTGCGGTACCCGGTCAGTACAAAGGGGAA
  GAACGCTCCCACTACTGCCGGCTTCAGCTATGATAAGTGGGAGATTAACCCATCAGAGCTGACCTTTATG
  AGAGAGTTGGGGAGCGGACTGTTTGGAGTGGTGAGGCTTGGCAAGTGGCGGGCCCAGTACAAAGTGGCCA
  TCAAAGCTATCCGGGAAGGCGCCATGTGTGAAGAGGATTTCATAGAGGAAGCTAAAGTCATGATGAAGCT
  GACACACCCCAAGCTGGTACAGCTCTATGGTGTATGCACCCAGCAGAAGCCCATCTACATCGTTACCGAG
  TTCATGGAACGGGGCTGCCTTCTGAATTTCCTCCGGCAGAGACAAGGCCATTTCAGCAGAGACATGCTGC
  TAAGCATGTGTCAAGATGTCTGTGAAGGGATGGAGTACCTGGAGAGAAACAGCTTCATCCACAGAGACCT
  GGCTGCCAGAAATTGTCTAGTGAATGAAGCAGGAGTTGTCAAAGTATCTGATTTTGGAATGGCCAGGTAC
  GTTCTGGATGATCAGTACACAAGTTCTTCTGGCGCCAAGTTCCCTGTGAAGTGGTGTCCCCCAGAAGTGT
  TTAATTACAGCCGCTTTAGCAGCAAGTCAGACGTCTGGTCGTTTGGTGTGCTAATGTGGGAAATATTCAC
  AGAAGGCAGGATGCCCTTTGAGAAGAACACCAATTACGAAGTGGTAACCATGGTGACTCGTGGCCACCGC
  CTCCACCGGCCAAAGCTGGCTTCCAAATATTTGTATGAGGTGATGCTGAGATGCTGGCAAGAGAGACCAG
  AGGGAAGGCCTTCCTTTGAAGACTTGCTGCGTACGATAGATGAACTAGTTGAATGTGAAGAAACTTTTGG
  AAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209373 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNFNTILEEILIKRSQQKKKTSPLNYKERLFVLTKSVLSYYEGRAEKKYRKGVIDISKIKCVEIVKNDDG
  VIPCQNKFPFQVVHDANTLYIFAPSPQSRDRWVKKLKEEIKNNNNIMIKYHPKFWADGSYQCCRQTEKLA
  PGCEKYNLFESSIRKTLPPAPEIKKRRPPPPIPPEEENTEEIVVAMYDFQATEAHDLRLERGQEYIILEK
  NDLHWWRARDKYGWYCRNTNRSKAEQLLRTEDKEGGFMVRDSSQPGLYTVSLYTKFGGEGSSGFRHYHIK
  ETATSPKKYYLAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSTKGKNAPTTAGFSYDKWEINPSELTFM
  RELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKLTHPKLVQLYGVCTQQKPIYIVTE
  FMERGCLLNFLRQRQGHFSRDMLLSMCQDVCEGMEYLERNSFIHRDLAARNCLVNEAGVVKVSDFGMARY
  VLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEIFTEGRMPFEKNTNYEVVTMVTRGHR
  LHRPKLASKYLYEVMLRCWQERPEGRPSFEDLLRTIDELVECEETFGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113461
• ORF Size: 1827 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113461.1, NM_001113461.2, NP_001106932.1
• RefSeq Size: 2595 bp
• RefSeq ORF: 1827 bp
• Locus ID: 21682
• MW: 71 kDa