Nr1d1 (NM_145434) Mouse Tagged ORF Clone

CAT#: MR209431

Nr1d1 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 1, group D, member 1 (Nr1d1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nr1d1
• Synonyms: A530070C09Rik; R75201
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209431 representing NM_145434
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACCCTGGACTCCAATAACAACACAGGTGGTGTTATCACCTACATTGGCTCTAGTGGCTCCTCCC
  CGAGCCGGACCAGCCCGGAGTCCCTCTACAGTGACAGCTCCAATGGCAGCTTCCAGTCCCTGACTCAAGG
  TTGTCCCACATACTTCCCACCATCACCTACTGGCTCCCTCACCCAGGACCCTGCCCGCTCTTTTGGCAGT
  GCGCCACCCAGTCTCAGTGATGATAGCTCCCCTTCTTCTACATCATCGTCATCCTCTTCATCCTCCTCCT
  CCTTCTATAACGGGAGCCCCCCAGGAAGTCTACAAGTGGCCATGGAAGACAGCAGCCGAGTGTCCCCCAG
  CAAGGGCACAAGCAACATTACCAAGCTGAATGGCATGGTGCTACTGTGTAAGGTGTGTGGGGACGTGGCC
  TCAGGCTTCCACTATGGAGTGCACGCCTGTGAGGGCTGCAAGGGCTTTTTTCGCCGGAGCATCCAACAGA
  ATATCCAGTACAAACGGTGTCTGAAAAACGAAAACTGCTCCATCGTTCGCATCAATCGCAACCGCTGCCA
  GCAGTGTCGCTTCAAGAAGTGTCTCTCCGTTGGCATGTCTAGAGATGCTGTGCGTTTTGGGCGCATCCCC
  AAGAGAGAGAAGCAACGGATGCTTGCCGAGATGCAGAGCGCCATGAACTTGGCCAACAACCAACTGAGCA
  GCCTGTGCCCTCTAGAGACCTCACCTACTCCACATCCCACCTCTGGCTCCATGGGCCCCTCACCTCCTCC
  TGCACCAGCCCCCACACCTTTGGTGGGCTTCTCTCAGTTCCCACAACAGCTGACACCACCCAGGTCTCCC
  AGCCCTGAGCCCACCATGGAGGATGTGATATCCCAGGTCGCCCGGGCCCATCGAGAAATCTTCACCTATG
  CCCATGACAAGTTAGGCACCTCACCTGGTAACTTCAATGCCAATCATGCATCAGGTAGCCCTTCAGCTAC
  AACTCCACACCGCTGGGAGAGTCAGGGATGCCCGTCTGCCCCCAACGACAACAACCTTTTGGCGGCTCAG
  CGTCATAATGAAGCGCTGAATGGTCTACGCCAGGGCCCTTCCTCCTACCCGCCTACCTGGCCTTCTGGCC
  CCACCCACCACAGCTGCCACCAACCTAACAGCAATGGGCACCGTCTGTGCCCCACCCACGTATATTCGGC
  CCCAGAAGGGGAGGCACCTGCCAACAGTCTACGGCAAGGCAACACCAAGAATGTTCTGCTGGCATGTCCC
  ATGAACATGTATCCCCATGGACGCAGCGGCCGGACTGTGCAGGAGATCTGGGAAGACTTCTCTATGAGCT
  TCACGCCTGCCGTTCGGGAGGTGGTAGAGTTTGCCAAACACATCCCAGGCTTCCGTGACCTTTCTCAGCA
  CGACCAGGTGACCCTGCTTAAGGCTGGCACCTTTGAGGTGCTGATGGTGCGCTTTGCATCGTTGTTCAAC
  GTGAAGGACCAGACAGTGATGTTCCTGAGCCGCACAACCTACAGTCTGCAGGAGCTCGGTGCCATGGGCA
  TGGGCGACCTGCTCAATGCCATGTTTGACTTCAGCGAGAAGCTCAACTCCCTGGCACTTACCGAGGAGGA
  GCTGGGCCTATTCACCGCGGTGGTGCTTGTCTCTGCAGACCGCTCGGGAATGGAGAATTCCGCTTCGGTG
  GAGCAGCTCCAGGAGACGCTGCTGCGGGCTCTTCGGGCTCTGGTGCTGAAGAACCGGCCCTCGGAGACTT
  CCCGCTTCACCAAGCTGCTGCTCAAGCTGCCGGACCTGCGGACCCTGAACAACATGCATTCCGAGAAGCT
  GCTGTCCTTCCGGGTGGACGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209431 representing NM_145434
  Red=Cloning site Green=Tags(s)
  
  MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDSSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGS
  APPSLSDDSSPSSTSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKGTSNITKLNGMVLLCKVCGDVA
  SGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIP
  KREKQRMLAEMQSAMNLANNQLSSLCPLETSPTPHPTSGSMGPSPPPAPAPTPLVGFSQFPQQLTPPRSP
  SPEPTMEDVISQVARAHREIFTYAHDKLGTSPGNFNANHASGSPSATTPHRWESQGCPSAPNDNNLLAAQ
  RHNEALNGLRQGPSSYPPTWPSGPTHHSCHQPNSNGHRLCPTHVYSAPEGEAPANSLRQGNTKNVLLACP
  MNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFN
  VKDQTVMFLSRTTYSLQELGAMGMGDLLNAMFDFSEKLNSLALTEEELGLFTAVVLVSADRSGMENSASV
  EQLQETLLRALRALVLKNRPSETSRFTKLLLKLPDLRTLNNMHSEKLLSFRVDAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145434
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145434.1, NM_145434.2, NM_145434.3, NM_145434.4, NP_663409.2
• RefSeq Size: 2627 bp
• RefSeq ORF: 1848 bp
• Locus ID: 217166
• MW: 67.3 kDa
• Gene Summary: This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (Arntl). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Feb 2014]