Trim25 (BC006908) Mouse Tagged ORF Clone

CAT#: MR209551

Trim25 (Myc-DDK-tagged) - Mouse tripartite motif protein 25 (cDNA clone MGC:6886 IMAGE:2651664)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim25
• Synonyms: EFP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209551 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGAATCCTCTGGCCGAGGAGCTGTCCTGCTCCGTCTGCCTGGAGCTCTTCAAGGAACCTG
  TCACCACCCCGTGCGGCCACAACTTCTGCATGTCATGCCTGGATGAGACGTGGGTCGTCCAGGGCCCGCC
  GTACCGGTGCCCGCAGTGTCGCAAAGTGTACCAGGTGCGCCCGCAGCTGCAAAAGAACACGGTAATGTGC
  GCGGTGGTGGAGCAGTTCCTGCAGGCTGAGCAGGCGCGGACCCCGGTGAACGACTGGACGCCCCCTGCCC
  GCTCCTCCGCTTCCAGCGCAGCCACCCAGGTGGCCTGCGACCACTGCCTGACGGAGATCGCAGTGAAGAC
  GTGCCTCGTGTGCATGGCCTCTTTCTGCCAGGAGCACTTGAGGCCTCACTTCGACAGTCCGGCCTTCCAG
  GATCACCCCCTGCAGTCGCCCATCCGTGACCTTCTGCGCCGCAAATGTACCCAGCACAACCGCCTTCGGG
  AATTGTTTTGTCCCGAGCATGGCGAGTGCATCTGCCATATCTGCTTGGTAGAGCACAAGACCTGCTCCCC
  TACGACCCTAAGTCAAGCCAGCGCCGACCTGGAGTACAAACTGAGGAATAAACTCACTATCATGCACAGC
  CACATCAATGGGGCAACCAAAGCATTGGAGGATGTGAGATCCAAGCAACAGTGTGTGCAGGATTCTATGA
  AGAGGAAGATGGAACAGCTGAGACAGGAGTATATGGAAATGAAGGCTGTCATCGACGCAGCAGAGACCAG
  CTCCTTGCGCAGGTTGAAGGAGGAGGAGAAGAGAGTCTACGGAAAATTCGACACCATCTACCAGGTTCTC
  GTCAAGAAGAAGAGTGAGATGCAGAAACTGAAGGCAGAGGTTGAGCTCATTATGGACAAAGGGGATGAGT
  TTGAGTTTCTGGAGAAAGCTGCAAAATTGCAAGGAGAGTCAACAAAACCAGTCTACATCCCTAAGATAGA
  TCTGGATCATGATTTGATAATGGGAATCTACCAAGGGGCGGCGGACCTCAAGAGTGAACTGAAGCACTCC
  ATTAAGAAGCTACAGAAGAAGTCTGAGGAGCACAATGGCTCAGGTAACAAGGGAGACCAGACACAGTCCA
  CTTTTAAACCCGTACAACCTTCGAAGAAGACCATTCCTGTTGCTCCCGGCTCTCCCTCCCACTTCTCACC
  TAACAAGTTGCCCACCTTTGGAGCTCCTGGCCAATCACTGGATTCAAAAGCAACTTCCCCTGATGCCGCA
  CCCAAAGCCAGTGCTGCACAGCCAGACTCTGTAGGAGTCAAGGCTAAGGTGCTAGAAAACTTCTTAACCA
  AGTCCAGAACGGAGCTTCTGGAGTATTTCGTGAAAGTCATCTTCGACTACAATACCGCCCACAACAAAGT
  GTCTCTGTCAAACAAGTACACCACCGCCTCTGTGTCTGATGGGCTCCAGCACTACCGGTCCCATCCCCAG
  AGGTTCACCTACTGCTCTCAGGTCCTGGGGCTGCACTGCTACAAGAACGGCATTCACTACTGGGAGGTGG
  AACTACAGAAGAACAACTTCTGCGGCGTAGGCATCTGCTACGGCAGCATGGAACGGCAGGGCCCCGAGAG
  CCGGCTGGGCCGCAACCCCAACTCATGGTGTGTGGAGTGGTTCAATAACAAGATCTCCGCCTGGCACAAC
  AACGTGGAGAAGACTCTGCCCTCCACCAAGGCCACTCGAGTCGGCGTGCTGCTCAACTGTGACCACGGCT
  TCGTCATTTTCTTTGCGGTCACCGAAAAGGTGCACCTGATGTACAAGTTCAAGGTGGACTTCACCGAAGC
  TCTGTACCCAGCCTTCTGGGTGTTCTCGGCCGGTACCACCCTCTCTATCTGCTCCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209551 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAELNPLAEELSCSVCLELFKEPVTTPCGHNFCMSCLDETWVVQGPPYRCPQCRKVYQVRPQLQKNTVMC
  AVVEQFLQAEQARTPVNDWTPPARSSASSAATQVACDHCLTEIAVKTCLVCMASFCQEHLRPHFDSPAFQ
  DHPLQSPIRDLLRRKCTQHNRLRELFCPEHGECICHICLVEHKTCSPTTLSQASADLEYKLRNKLTIMHS
  HINGATKALEDVRSKQQCVQDSMKRKMEQLRQEYMEMKAVIDAAETSSLRRLKEEEKRVYGKFDTIYQVL
  VKKKSEMQKLKAEVELIMDKGDEFEFLEKAAKLQGESTKPVYIPKIDLDHDLIMGIYQGAADLKSELKHS
  IKKLQKKSEEHNGSGNKGDQTQSTFKPVQPSKKTIPVAPGSPSHFSPNKLPTFGAPGQSLDSKATSPDAA
  PKASAAQPDSVGVKAKVLENFLTKSRTELLEYFVKVIFDYNTAHNKVSLSNKYTTASVSDGLQHYRSHPQ
  RFTYCSQVLGLHCYKNGIHYWEVELQKNNFCGVGICYGSMERQGPESRLGRNPNSWCVEWFNNKISAWHN
  NVEKTLPSTKATRVGVLLNCDHGFVIFFAVTEKVHLMYKFKVDFTEALYPAFWVFSAGTTLSICSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC006908
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC006908, AAH06908
• RefSeq Size: 2602
• RefSeq ORF: 1880
• Locus ID: 217069
• MW: 70.7 kDa