Kcnc4 (NM_145922) Mouse Tagged ORF Clone

CAT#: MR209572

Kcnc4 (Myc-DDK-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 4 (Kcnc4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnc4
• Synonyms: AI850292; Kcr2-4; KSHIIIC; Kv3.4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209572 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAGCTCGGTGTGTGTCTCCTCCTACCGCGGGCGCAAGTCGGGGAACAAGCCTCCGTCCAAAACAT
  GTCTGAAGGAGGAGATGGCCAAGGGCGAGGCGTCGGAGAAGATCATCATCAACGTGGGCGGCACGCGACA
  TGAGACCTACCGCAGCACCCTGCGCACCCTACCGGGCACCCGCCTTGCCTGGTTGGCGGATCCCGACGGC
  GGGGGTCGGCCAGAGTCGGATGGCGGCGGTGCAGGCAGCAGCGGCAGCAGCGGCGGCGGCGGCGGCGGCG
  GGGGCTGTGAGTTCTTCTTTGATCGGCACCCGGGTGTTTTTGCCTATGTGCTCAACTACTACCGTACGGG
  CAAGCTGCATTGCCCCGCAGACGTCTGTGGGCCTCTCTTTGAGGAAGAGCTCACCTTCTGGGGTATCGAT
  GAAACAGATGTGGAACCCTGCTGCTGGATGACCTACCGGCAGCACCGCGATGCTGAGGAGGCACTGGACA
  TCTTCGAGAGCCCGGACGGGGGCGGGGGTGGCGCAGGGCCAGGCGACGAGGCTGGCGACGATGAGCGGGA
  GTTGGCCTTGCAGCGCCTGGGCCCCCATGAAGGAGGCTCTGGCCCTGGTGCTGGGTCCGGAGGGTGCCGT
  GGCTGGCAGCCCCGAATGTGGGCGCTCTTCGAGGATCCCTACTCATCCCGGGCGGCCAGGGTGGTAGCCT
  TTGCCTCTCTCTTCTTCATCTTGGTCTCCATTACCACCTTCTGCCTGGAGACCCATGAGGCCTTCAACAT
  TGACCGAAACGTGACGGAGATTCATCGGGTAGGGAATATCACCAGCGTGCGCTTCCGGCGGGAGGTAGAA
  ACAGAGCCCATTCTTACCTACATCGAGGGTGTGTGCGTGATGTGGTTCACTCTAGAGTTCCTGGTTCGCA
  TTGTGTGCTGCCCTGATACGCTGGACTTTGTCAAGAACTTGCTCAACATCATCGACTTTGTGGCCATCTT
  ACCCTTTTACCTGGAGGTGGGATTGAGTGGTTTGTCTTCCAAGGCAGCTCGCGATGTGCTGGGTTTCCTG
  CGTGTGGTACGCTTTGTACGCATCCTGCGGATCTTCAAGCTCACACGCCACTTTGTGGGGCTGCGTGTGC
  TAGGCCACACTCTCCGGGCCAGCACTAATGAGTTCCTGTTGCTTATCATCTTCTTGGCCCTGGGTGTGCT
  CATCTTCGCCACCATGATCTATTATGCCGAGCGAATTGGGGCCAGGCCATCTGACCCACGGGGCAACGAC
  CACACCGACTTCAAGAACATCCCCATCGGTTTCTGGTGGGCTGTGGTCACCATGACAACGCTTGGCTATG
  GGGACATGTATCCCAAGACGTGGTCAGGAATGTTGGTGGGTGCGCTGTGTGCGCTGGCTGGTGTGCTGAC
  CATTGCCATGCCCGTGCCTGTCATCGTCAATAACTTTGGTATGTACTACTCCCTGGCTATGGCCAAGCAG
  AAGCTGCCCAAGAAGCGAAAGAAGCACGTACCACGGCCACCCCAGCTCGAATCGCCCATTTACTGCAAGT
  CTGAGGAGACTTCACCCCGGGACAGCACCTACAGTGACACCAGCCCCCCTGCCCGGGAAGAGGGTGTGGT
  CGAGAGGAAACGGGCCGACTCTAAGCAGAATGGCGATGCTAATGCGGTGCTGTCTGATGAGGAGGGAGCT
  GGCCTCACTCAGCCCTTGGCCTTGGCCCCCACCCCTGAAGAGCGTCGGGCCCTGAGACGCTCAGGCACGC
  GGGACAGAAACAAGAAGGCAGCTGCCTGCTTCCTGCTCAGTGCTGGGGACTATGCCTGTGCTGATGGCAG
  TGTCCGGAAAGAAGGCAATGTTGAGCCGAAAGCGTGCGTCCCAGTGTCTCACACCTGTGCTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209572 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MISSVCVSSYRGRKSGNKPPSKTCLKEEMAKGEASEKIIINVGGTRHETYRSTLRTLPGTRLAWLADPDG
  GGRPESDGGGAGSSGSSGGGGGGGGCEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFEEELTFWGID
  ETDVEPCCWMTYRQHRDAEEALDIFESPDGGGGGAGPGDEAGDDERELALQRLGPHEGGSGPGAGSGGCR
  GWQPRMWALFEDPYSSRAARVVAFASLFFILVSITTFCLETHEAFNIDRNVTEIHRVGNITSVRFRREVE
  TEPILTYIEGVCVMWFTLEFLVRIVCCPDTLDFVKNLLNIIDFVAILPFYLEVGLSGLSSKAARDVLGFL
  RVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGARPSDPRGND
  HTDFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAKQ
  KLPKKRKKHVPRPPQLESPIYCKSEETSPRDSTYSDTSPPAREEGVVERKRADSKQNGDANAVLSDEEGA
  GLTQPLALAPTPEERRALRRSGTRDRNKKAAACFLLSAGDYACADGSVRKEGNVEPKACVPVSHTCAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145922
• ORF Size: 1887 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145922.1, NM_145922.2, NP_666034.1
• RefSeq Size: 3013 bp
• RefSeq ORF: 1887 bp
• Locus ID: 99738
• MW: 68.7 kDa