Racgap1 (BC010715) Mouse Tagged ORF Clone

CAT#: MR209580

Racgap1 (Myc-DDK-tagged) - Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Racgap1
• Synonyms: Band25, GTPase, MgcRacGAP, gtl11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209580 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACTACAATGGTGAATTTGTGGACTCTGTTTGAGCAGCTTGTGCGCCGGATGGAGATTATCAATG
  AAGGAAATGAAAGCATTGAATTCATCCAGGTTGTGAAGGACTTCGAGGACTTCCGAAAGAAGTATCAAAG
  AACCAACCAGGAGCTGGAGAAATTCAAAGACCTATTGTTGAAAGCAGAGACGGGGCGGAGCGCCCTGGAC
  GTGAAGCTGAAGCATGCCCGTAATCAAGTGGACGTGGAGATCAAGCGGAGGCAGCGCGCTGAGGCAGAGT
  GTGCAAAGCTGGAACAACAGATTCAGCTGATTCGAGACATACTCATGTGTGACACATCTGGCAGTATTCA
  GCTGAGCGAGGAACAAAAATCAGCTCTCGCTTTCCTCAACCGAGGCCAAGCATCCAGTGGCCACGCCGGC
  AACAATAGACTGTCAACGATTGATGAATCTGGTTCCATTTTATCAGATATCAGCTTTGACAAGACTGATG
  AATCACTGGACTGGGATTCTTCTTTGGTGAAGAATTTCAAAATGAAGAAACGAGAGAAGAGGCGCTCCAA
  CAGTAGACAGTTCATCGACGGCCCTCCTGGGCCTGTGAAGAAAACTTGTTCCATTGGCTCTACAGTAGAC
  CAGGCAAATGAATCAATAGTTGCAAAAACTACAGTGACTGTTCCCAGTGATGGGGGACCCATTGAAGCTG
  TGTCTACTATTGAGACATTGCCGTCCTGGACCAGGAGTCGCGGGAAGTCAGGACCTTTACAACCTGTGAA
  CAGTGACTCCGCTCTGAACAGCAGGCCACTGGAGCCAAGAACTGACACAGACAATTTGGGCACACCTCAG
  AATACTGGAGGCATGCGCTTGCACGACTTCGTCTCAAAGACGGTTATTAAGCCTGAATCTTGTGTTCCGT
  GTGGAAAGCGGATCAAGTTTGGCAAGCTGTCTCTGAAGTGTCGAGACTGTCGTTTGGTCTCCCATCCAGA
  ATGTCGGGACCGATGTCCCCTTCCCTGCATCCCCCCCCTGGTGGGGACACCGGTTAAGATTGGAGAGGGC
  ATGCTGGCCGACTTCGTGTCGCAGGCTTCTCCCATGATCCCTGCCATTGTCGTCAGCTGTGTCAATGAGA
  TCGAGCAGCGAGGCCTGACTGAGGCAGGCTTGTACAGGATCTCAGGCTGTGACCGCACAGTGAAAGAACT
  GAAAGAAAAATTCCTTAAGGTGAAAACTGTGCCCCTCCTCAGCAAAGTGGACGATATCCACGTCATCTGC
  AGCCTCCTGAAGGACTTCCTGCGCAACCTCAAAGAGCCCCTCCTGACCTTCTGGCTGAGCAAAGCCTTCA
  TGGAGGCAGCAGAGATAACAGATGAAGACAACAGCACAGCCGCCATGTACCAGGCTGTCAGTGAGCTGCC
  CCAGGCCAACAGGGACACGCTAGCCTTCCTTATGATCCACCTACAGAGAGTGTCTCAGAGTCCAGACACT
  AAGATGGATATTGCCAATCTAGCTAAAGTCTTTGGCCCTACAATAGTTGCCCATACTGTGCCCAATCCAG
  ATCCAGTGACAATGTTCCAGGACATCAAACGTCAGCTCAAGGTGGTGGAGCGCCTACTCTCTCTCCCCTT
  GGAGTACTGGAATCAGTTCATGATGGTGGACCAAGAGAACATAGACAGCCAGCGAGGCAATGGAAACTCA
  ACACCACGCACACCAGACGTTAAAGTGAGCTTACTGGGGCCTGTGACCACTCCTGAATTCCAGCTTGTCA
  AGACTCCTTTATCAAGTTCCCTGTCACAGAGGTTGTACAACCTCTCCAAGAGCACACCCAGATTTGGGAA
  CAAGAGCAAGTCTGCCACCAACTTAGGTCAACAGGGCAAATTTTTCCCTGCTCCGTACCTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209580 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDTTMVNLWTLFEQLVRRMEIINEGNESIEFIQVVKDFEDFRKKYQRTNQELEKFKDLLLKAETGRSALD
  VKLKHARNQVDVEIKRRQRAEAECAKLEQQIQLIRDILMCDTSGSIQLSEEQKSALAFLNRGQASSGHAG
  NNRLSTIDESGSILSDISFDKTDESLDWDSSLVKNFKMKKREKRRSNSRQFIDGPPGPVKKTCSIGSTVD
  QANESIVAKTTVTVPSDGGPIEAVSTIETLPSWTRSRGKSGPLQPVNSDSALNSRPLEPRTDTDNLGTPQ
  NTGGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRLVSHPECRDRCPLPCIPPLVGTPVKIGEG
  MLADFVSQASPMIPAIVVSCVNEIEQRGLTEAGLYRISGCDRTVKELKEKFLKVKTVPLLSKVDDIHVIC
  SLLKDFLRNLKEPLLTFWLSKAFMEAAEITDEDNSTAAMYQAVSELPQANRDTLAFLMIHLQRVSQSPDT
  KMDIANLAKVFGPTIVAHTVPNPDPVTMFQDIKRQLKVVERLLSLPLEYWNQFMMVDQENIDSQRGNGNS
  TPRTPDVKVSLLGPVTTPEFQLVKTPLSSSLSQRLYNLSKSTPRFGNKSKSATNLGQQGKFFPAPYLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC010715
• ORF Size: 1884 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010715, AAH10715
• RefSeq Size: 2913 bp
• RefSeq ORF: 1886 bp
• Locus ID: 26934
• MW: 70.2 kDa