Jph4 (BC052049) Mouse Tagged ORF Clone

CAT#: MR209581

Jph4 (Myc-DDK-tagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Jph4
• Synonyms: JP-4, JPHL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209581 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCCGGGGGCAAGTTCGACTTTGACGACGGGGGCTGCTACGTGGGGGGCTGGGAGGCGGGGCGGG
  CACATGGCTACGGCGTGTGCACCGGGCCCGGCGCCCAGGGAGAGTACAGCGGCTGCTGGGCTCACGGCTT
  CGAGTCGCTGGGTGTCTTCACGGGGCCCGGCGGACACAGTTACCAGGGCCACTGGCAGCAGGGCAAGCGC
  GAAGGGCTGGGCGTGGAGCGCAAGAGCCGCTGGACCTACCGCGGCGAGTGGCTGGGCGGGCTGAAGGGGC
  GCAGCGGCGTATGGGAGAGCGTGTCCGGCCTGCGCTACGCCGGGCTCTGGAAGGACGGCTTCCAGGACGG
  CTACGGCACCGAGACCTACTCGGATGGAGGCACTTACCAAGGCCAGTGGCAAGCCGGGAAACGCCACGGC
  TACGGGGTGCGCCAAAGTGTACCCTACCACCAGGCGGCGCTTCTGCGCTCACCCCGCCGCACCTCCCTGG
  ACTCCGGCCACAGCGACCCCCCGACGCCGCCTCCACCCCTACCCCTACCAGGAGATGAAGGAGGCAGCCC
  AGCCTCCGGCTCCCGAGGCGGCTTCGTGCTGGCGGGGCCAGGGGACGCCGACGGGGCGTCTTCCCGTAAG
  CGCACCCCAGCAGCAGGTGGATTCTTCCGCCGTTCACTGCTGCTCAGCGGGCTCCGGGCTGGTGGGCGCC
  GCAGCTCCCTGGGCAGCAAGAGGGGATCTCTACGCAGCGAGGTGAGCAGCGAAGTGGGCAGTACAGGACC
  CCCGGGCTCCGAGGCCAGCGGGCCCCCAATCCCAGCTCCACCTGCCCTCATCGAGGGCTCAGCCACTGAG
  GTGTACGCCGGTGAGTGGCGTGCGGACCGGCGCAGTGGCTATGGAGTGAGCCAGAGATCCAACGGGCTGC
  GCTACGAAGGCGAGTGGCTGGGCAACCGACGCCACGGATACGGGCGCACCACCCGGCCCGATGGCTCCCG
  TGAGGAGGGCAAGTACAAGCGTAACCGGCTGGTACACGGGGGACGTGTCCGCAGCCTCCTGCCTCTGGCC
  CTTAGACGGGGCAAAGTCAAGGAGAAAGTGGACAGGGCTGTCGAAGGTGCCCGTCGAGCTGTGAGTGCTG
  CCCGCCAGCGCCAGGAAATTGCGGCTGCCAGGGCAGCAGACGCCCTCCTAAAAGCAGTGGCAGCCAGCAG
  CGTCGCGGAGAAGGCTGTGGAGGCTGCGCGGATGGCCAAGCTGATAGCCCAGGATCTACAACCCATGTTA
  GAGGCCCCAGGCCGCAGACCCAGACAGGACTCAGGAGGTTCTGACACAGAGCCTTTGGATGAGGACAGCC
  CTGGGGTATACGAGAATGGACTGACCCCCTCAGAGGGCTCTCCGGAACTACCCAGCAGCCCCGCCTCCTC
  CCACCAACCTTGGCGAGCCCCTGCCTGCCGGAGTCCACTGCCTCCTGGAGGGAACTGGGGCCCCTTCTCC
  AGCCCTAAAGCTTGGCCCGAGGAGTGGGGGGGTCCTGGTGAGCAGGCAGAGGAACTAGCTGGCTATGAGG
  CTGAGGATGAGGCTGGGGTGCAGGGTCCAGGACCAAGAGATGGTTCCCCACTTCTCGGAGGCTGCAGTGA
  CAGTTCAGGAAGTCTTCGAGAGGAGGAGGGGGAAGACGAAGAGTCCTTGCCCCAACTGAGGGCCCCAGGA
  GGCTCAGAGTCTGAGCCTGTCACCACGCCAGTTTTGAGGGGCCTGTCTTCAAGGGGTCCTGATGCTGGGT
  GCCTGACAGAAGAGGTTGAGGAGCCTGCTCCCACCGAGAGGCCTGCCCAGCCGGGAGCTGCCAACCCCCT
  GGTGGTGGGAGCCGTGGCCTTGCTGGACCTCAGCCTGGCCTTCCTGTTCTCCCAGCTCCTCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209581 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSPGGKFDFDDGGCYVGGWEAGRAHGYGVCTGPGAQGEYSGCWAHGFESLGVFTGPGGHSYQGHWQQGKR
  EGLGVERKSRWTYRGEWLGGLKGRSGVWESVSGLRYAGLWKDGFQDGYGTETYSDGGTYQGQWQAGKRHG
  YGVRQSVPYHQAALLRSPRRTSLDSGHSDPPTPPPPLPLPGDEGGSPASGSRGGFVLAGPGDADGASSRK
  RTPAAGGFFRRSLLLSGLRAGGRRSSLGSKRGSLRSEVSSEVGSTGPPGSEASGPPIPAPPALIEGSATE
  VYAGEWRADRRSGYGVSQRSNGLRYEGEWLGNRRHGYGRTTRPDGSREEGKYKRNRLVHGGRVRSLLPLA
  LRRGKVKEKVDRAVEGARRAVSAARQRQEIAAARAADALLKAVAASSVAEKAVEAARMAKLIAQDLQPML
  EAPGRRPRQDSGGSDTEPLDEDSPGVYENGLTPSEGSPELPSSPASSHQPWRAPACRSPLPPGGNWGPFS
  SPKAWPEEWGGPGEQAEELAGYEAEDEAGVQGPGPRDGSPLLGGCSDSSGSLREEEGEDEESLPQLRAPG
  GSESEPVTTPVLRGLSSRGPDAGCLTEEVEEPAPTERPAQPGAANPLVVGAVALLDLSLAFLFSQLLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052049
• ORF Size: 1884 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052049.1
• RefSeq Size: 3556 bp
• RefSeq ORF: 1886 bp
• Locus ID: 319984
• MW: 66 kDa