Kirrel1 (BC023765) Mouse Tagged ORF Clone

CAT#: MR209638

Kirrel1 (Myc-DDK-tagged) - Mouse kin of IRRE like 1 (Drosophila) (cDNA clone MGC:38329 IMAGE:5343720)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kirrel1
• Synonyms: 6720469N11Rik|Kirrel1|Neph1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209638 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTGGAGAGCCCTAGCACACGTCTAATGACCTGCCAATCAAGCCTGCTCCCTGAGAAGCCCAGGT
  TTCTGAGTCAGAAGATGTGGGCTCCACACTTAGTCGTTGCTTATCTTATCTTTGTGACTTTGGCTTTGGC
  TTTGCCCGGGACTCAGACTCGCTTCAGCCAGGAGCCAGCTGATCAGACTGTGGTGGCCGGACAGCGGGCG
  GTGCTCCCGTGTGTGCTCCTTAACTACTCTGGGATTGTACAGTGGACCAAGGACGGGCTGGCCCTGGGTA
  TGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTCGTGGGCTCTGCGGATGCTGGGCAATACAACTT
  GGAGATCACAGATGCCGAGCTGTCTGATGACGCTTCCTATGAGTGCCAGGCCACGGAGGCTGCCCTGCGC
  TCTCGGCGGGCCAAACTCACCGTGCTCATTCCTCCAGAGGAAACAAGGATTGATGGGGGCCCGGTGATTC
  TGCTGCAAGCAGGCACCCCCTACAACCTCACGTGCAGAGCATTTAATGCCAAACCTGCTGCCACCATCAT
  TTGGTTCCGAGATGGGACACAGCAGGAGGGGGCTGTGACTAGCACGGAGCTGCTGAAGGATGGGAAAAGG
  GAGACCACAATCAGCCAACTGCTCATTGAGCCCACAGACCTAGACATTGGCCGCGTATTCACCTGTCGCA
  GTATGAATGAGGCCATCCCCAATGGCAAGGAGACATCCATTGAGCTTGATGTGCACCACCCTCCCACAGT
  GACTCTGTCCATCGAGCCCCAGACAGTGCTGGAAGGCGAGCGTGTCATTTTTACATGCCAGGCCACAGCC
  AACCCAGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGCTTCTTGATTGAAGACGCCCATGAGAGTCGCT
  ATGAGACAAACGTTGACTATTCCTTCTTCACGGAGCCTGTGTCTTGTGAGGTTTATAACAAAGTCGGGAG
  CACCAATGTCAGCACTTTAGTGAATGTTCACTTCGCCCCCCGGATTGTAGTTTACCCAAAGCCCACCACC
  ACAGACATTGGATCTGATGTGACCCTCACTTGTGTCTGGGTTGGGAATCCTCCCCTCACCCTCACCTGGA
  CCAAGAAGGACTCAAACATGGTCCTGAGTAACAGCAATCAACTGTTGCTGAAGTCAGTGACCCAGGCAGA
  TGCTGGCACCTATACCTGCCGGGCCATCGTGCCTCGGATCGGAGTGGCTGAGCGAGAGGTACCGCTTTAT
  GTAAACGGACCTCCTATCATCTCCAGCGAGGCGGTACAGTTTGCTGTGAGAGGTGATGGCGGTAAGGTGG
  AGTGCTTTATCGGGAGTACCCCACCTCCGGATCGAATTGCATGGGCATGGAAGGAGAACTTCCTCGAGGT
  GGGGACCCTGGAACGCTACACCGTGGAGAGGACGAACTCAGGCAGCGGTGTGCTGTCCACGCTCACCATT
  AATAATGTCATGGAGGCGGACTTCCAGACCCACTACAACTGCACTGCCTGGAACAGCTTTGGACCAGGCA
  CAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATTGCCGGGGCCACCATCGGTGC
  CGGCATCCTGGTCGTCTTCTCTTTTGCTGCCTTAGTGTTCTTCCTCTACCGACGTCGCAAAGGCAGTCGA
  AAGGATGTGACGTTGAGGAAGCTGGACATCAAGGTGGAGACGGTGAATCGGGAGCCACTTACGATGCACT
  CTGACCGGGAGGATGATACTGCCAGCATTTCCACGGCAACGCGGGTCATGAAGGCCATCTACTCGGTGAG
  GATAATGCTCCTTTCAACGTGGCCTAGGCTCTTCATTGTCCTACGGCCAGCCCCACCAGCTTTTAATGAT
  TTCCGTTACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209638 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTLESPSTRLMTCQSSLLPEKPRFLSQKMWAPHLVVAYLIFVTLALALPGTQTRFSQEPADQTVVAGQRA
  VLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALR
  SRRAKLTVLIPPEETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEGAVTSTELLKDGKR
  ETTISQLLIEPTDLDIGRVFTCRSMNEAIPNGKETSIELDVHHPPTVTLSIEPQTVLEGERVIFTCQATA
  NPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVYNKVGSTNVSTLVNVHFAPRIVVYPKPTT
  TDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLY
  VNGPPIISSEAVQFAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTI
  NNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGAGILVVFSFAALVFFLYRRRKGSR
  KDVTLRKLDIKVETVNREPLTMHSDREDDTASISTATRVMKAIYSVRIMLLSTWPRLFIVLRPAPPAFND
  FRYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC023765
• ORF Size: 1902 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023765, AAH23765
• RefSeq Size: 2228 bp
• RefSeq ORF: 1904 bp
• Locus ID: 170643
• MW: 70 kDa