Fzd1 (NM_021457) Mouse Tagged ORF Clone

CAT#: MR209705

Fzd1 (Myc-DDK-tagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fzd1
• Synonyms: AW227548; FZ-1; Fz1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209705 representing NM_021457
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGAGGCGGCGCCTAGCGAGTCCCGGGCCGCCGGCCGGCTGAGCTTGGAACTTTGTGCCGAAG
  CACTCCCGGGCCGGCGGGAGGAGGTGGGGCACGAGGACACGGCCAGCCACCGCCGCCCCCGGGCTGATCC
  CCGGCGTTGGGCTAGCGGGCTGCTGCTGCTGCTTTGGTTGCTGGAGGCTCCTCTGCTTTTGGGGGTCCGA
  GCGCAGGCGGCGGGCCAGGTATCCGGGCCGGGCCAGCAAGCCCCGCCGCCGCCCCAGCCCCAGCAGAGCG
  GGCAGCAGTACAACGGCGAACGGGGCATCTCCATCCCGGACCACGGCTACTGCCAGCCCATCTCCATCCC
  GCTGTGCACGGACATCGCGTACAACCAGACCATCATGCCCAACCTGCTGGGCCACACGAATCAGGAGGAC
  GCCGGTCTGGAGGTGCACCAGTTCTACCCTCTGGTGAAGGTGCAGTGCTCCGCCGAGCTCAAGTTCTTCC
  TGTGCTCCATGTACGCGCCTGTGTGCACCGTACTGGAGCAGGCGCTACCGCCCTGCCGCTCCCTGTGCGA
  GCGCGCACGCCAGGGCTGCGAGGCGCTCATGAACAAGTTCGGCTTCCAGTGGCCAGACACACTCAAGTGC
  GAGAAGTTCCCGGTGCACGGCGCAGGAGAGCTGTGCGTGGGCCAGAACACGTCCGACAAAGGCACCCCAA
  CTCCCTCCTTGCTACCAGAGTTCTGGACCAGTAATCCGCAGCACGGCGGCGGTGGTTACCGCGGCGGCTA
  CCCGGGGGGTGCCGGGACGGTGGAGCGGGGAAAGTTCTCCTGCCCGCGCGCCCTCAGGGTGCCCTCCTAC
  CTCAACTACCACTTTCTGGGGGAGAAGGACTGCGGCGCACCCTGCGAACCCACCAAGGTTTACGGGCTCA
  TGTACTTCGGGCCAGAGGAGCTGCGCTTCTCGCGCACCTGGATAGGCATCTGGTCCGTGCTGTGCTGCGC
  CTCCACGCTCTTCACGGTGCTCACGTACCTAGTGGACATGCGGCGCTTCAGCTACCCGGAACGGCCCATC
  ATTTTCCTGTCCGGCTGTTACACAGCGGTGGCGGTGGCCTACATCGCTGGCTTTCTGTTGGAGGACCGGG
  TGGTGTGCAACGACAAGTTTGCAGAGGACGGGGCGCGCACGGTGGCGCAGGGCACTAAGAAAGAAGGCTG
  CACTATACTCTTTATGATGCTCTACTTCTTCAGCATGGCCAGCTCCATCTGGTGGGTGATCCTGTCCCTC
  ACCTGGTTCCTGGCAGCCGGCATGAAGTGGGGCCACGAAGCCATCGAGGCCAACTCACAGTATTTCCATT
  TAGCCGCCTGGGCTGTGCCAGCCATCAAAACTATAACCATCTTGGCGTTGGGCCAGGTGGATGGCGACGT
  ACTGAGCGGAGTGTGTTTTGTGGGGCTCAACAACGTGGACGCACTGCGTGGCTTTGTGCTGGCGCCTCTC
  TTCGTTTATCTGTTCATTGGCACTTCTTTCCTGCTGGCCGGTTTCGTGTCACTCTTCCGCATCCGCACCA
  TCATGAAGCATGACGGCACCAAGACAGAGAAGCTGGAGAAGCTCATGGTGCGCATCGGAGTCTTCAGTGT
  CCTCTACACTGTGCCGGCCACCATCGTCATCGCCTGCTACTTCTATGAACAGGCCTTTCGGGACCAGTGG
  GAGCGCAGCTGGGTGGCCCAGAGCTGCAAGAGTTATGCCATCCCTTGCCCTCACCTCCAGGGAGGTGGAG
  GAGTCCCACCACACCCGCCCATGAGCCCAGACTTTACAGTCTTCATGATCAAGTATCTCATGACGCTGAT
  TGTGGGCATCACATCGGGCTTCTGGATCTGGTCCGGCAAGACACTGAATTCCTGGAGGAAGTTCTACACG
  AGGCTTACCAACAGCAAACAGGGGGAGACTACCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209705 representing NM_021457
  Red=Cloning site Green=Tags(s)
  
  MAEEAAPSESRAAGRLSLELCAEALPGRREEVGHEDTASHRRPRADPRRWASGLLLLLWLLEAPLLLGVR
  AQAAGQVSGPGQQAPPPPQPQQSGQQYNGERGISIPDHGYCQPISIPLCTDIAYNQTIMPNLLGHTNQED
  AGLEVHQFYPLVKVQCSAELKFFLCSMYAPVCTVLEQALPPCRSLCERARQGCEALMNKFGFQWPDTLKC
  EKFPVHGAGELCVGQNTSDKGTPTPSLLPEFWTSNPQHGGGGYRGGYPGGAGTVERGKFSCPRALRVPSY
  LNYHFLGEKDCGAPCEPTKVYGLMYFGPEELRFSRTWIGIWSVLCCASTLFTVLTYLVDMRRFSYPERPI
  IFLSGCYTAVAVAYIAGFLLEDRVVCNDKFAEDGARTVAQGTKKEGCTILFMMLYFFSMASSIWWVILSL
  TWFLAAGMKWGHEAIEANSQYFHLAAWAVPAIKTITILALGQVDGDVLSGVCFVGLNNVDALRGFVLAPL
  FVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLEKLMVRIGVFSVLYTVPATIVIACYFYEQAFRDQW
  ERSWVAQSCKSYAIPCPHLQGGGGVPPHPPMSPDFTVFMIKYLMTLIVGITSGFWIWSGKTLNSWRKFYT
  RLTNSKQGETTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021457
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021457.2, NM_021457.3, NP_067432.2
• RefSeq Size: 4395
• RefSeq ORF: 1929
• Locus ID: 14362
• MW: 71.6 kDa
• Gene Summary: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. [UniProtKB/Swiss-Prot Function]