Ndst3 (BC079622) Mouse Tagged ORF Clone

CAT#: MR209708

Ndst3 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (cDNA clone MGC:90637 IMAGE:30535990)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ndst3
• Synonyms: 4921531K01Rik|4930511P15Rik|N-HSST 3|NDST-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209708 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTTTATCATGAAACCTCACAGACACTTTCAGAGAACACTGATTCTGCTTGCCACCTTTTGTATGG
  TAAGCATTATTATTTCTGCTTACTACCTGTACAGCGGCTACAAACAGGAAAGTGAAGTCTCCGGGCGGGC
  TTCGGAAGTGGACTGTGGTGACCTCCAGCACATACCATCCAGGCTGATGGAAGTGAGGAGGACAATGATT
  TCCGATGCTTCAAGGACAGACCCCACAGTCCTGGTGTTTGTGGAGAGCCAGTACTCATCCCTTGGTCAAG
  ACATCATTATGATGCTAGAATCCATCCGGTTCCATTATCACACTGAAATCGCTCCTGGAAAAGGAGATCT
  TCCGGCACTTACAGACAATGTGAAGGGCAAATATGTTCTCATTATATATGAGAATATTCTAAAGTATATA
  AACATGGACTCTTGGAATAGAAGCCTTTTAGATAAATACTGTATAGAGTATGGTGTGGGTATCATTGGAT
  TCCATAAAACCAGTGAGAAAAATCTACAGAGCTTTCAGTTCAGGGGCTTCCCCTTTTCCATAAGTGGAAA
  CCTGGCAGTAAAAGATTGCTGTATTAATCCTCACTCCCCACTCCTTCGTGTGACCAAATCATCCAAGCTG
  GACAGAGGTTCTCTACCTGGAACTGACTGGACAGTTTTTCAGATTAACCACTCCACCTACCAGCCAGTAA
  TATTTGCCAAAGTTAAGACTCCAGAAAACCTTTCTCCTCCCATCTCTAAACATGCATTTTATGCCACTAT
  CATACACGACCTGGGGCTTCATGACGGGATCCAGCGAGTTCTTTTTGGCAACAACTTGAACTTCTGGCTA
  CACAAGCTCATCTTCATAGACGCCATCTCCTTCTTGTCGGGGAAGAGGCTGACACTGTCCTTGGACAGGT
  ACATCCTTGTGGACATTGACGACATATTTGTAGGGAAGGAGGGCACAAGGATGAACACCAATGATGTGAA
  GGCCCTGCTTGACACTCAGAATCTTTTGCGCACACAAATCACAAATTTTACATTCAACCTTGGATTTTCA
  GGGAAATTTTATCACACAGGAACTGAAGAGGAAGATGAAGGGGATGATTGTCTGCTGGGTTCTGTGGACG
  AGTTCTGGTGGTTTCCTCACATGTGGAGTCACATGCAGCCCCACCTCTTCCACAATGAGTCGTCTTTAAT
  AGAGCAGATGATTCTCAACAAAAAGTTTGCCTTAGAGCACGGCATCCCTACCGATATGGGATATGCAGTG
  TCCCCTCACCATTCAGGTGTCTACCCTGTCCATGTTCAGCTTTATGAGGCCTGGAAGAAGGTTTGGAATA
  TTAAAATCACCAGCACTGAAGAATATCCACATCTGAAACCAGCTAGATATCGGAGAGGCTTCATTCACAA
  AAACATCATGGTTCTCCCAAGACAAACCTGTGGGCTCTTCACCCACACAATTTTCTACAAGGAGTATCCA
  GGGGGTCCCAGGGAACTGGACAAGAGTATTCATGGAGGGGAGCTCTTCTTCACTGTGGTTCTCAACCCAA
  TCAGTATTTTCATGACCCATTTGTCTAACTATGGAAACGACCGACTGGGATTATATACATTTGTGAATCT
  AGCCAATTTTGTGCAGACCTGGACCAACCTGCGTCTTCAGACCTTGCCTCCAGCTCAGCTGGCTCACAAG
  TATTTTGAGCTCTTTCCTGATCAGAAAGACCCTCTCTGGCAGAACCCCTGTGATGACAAACGCCACAGAG
  ACATTTGGTCTAAAGAGAAAACCTGCGATCGTTTACCAAAATTCTTGGTAATAGGACCCCAGAAAACTGG
  TACCACTGCCTTGTGTCTGTTCCTGATTATGCATCCTTCCATCCTTAGTAACTCCCCCAGCCCAAAATCC
  TTTGAGGAGGTACATGGATTTCTTCCCAGTCCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209708 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFIMKPHRHFQRTLILLATFCMVSIIISAYYLYSGYKQESEVSGRASEVDCGDLQHIPSRLMEVRRTMI
  SDASRTDPTVLVFVESQYSSLGQDIIMMLESIRFHYHTEIAPGKGDLPALTDNVKGKYVLIIYENILKYI
  NMDSWNRSLLDKYCIEYGVGIIGFHKTSEKNLQSFQFRGFPFSISGNLAVKDCCINPHSPLLRVTKSSKL
  DRGSLPGTDWTVFQINHSTYQPVIFAKVKTPENLSPPISKHAFYATIIHDLGLHDGIQRVLFGNNLNFWL
  HKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNTNDVKALLDTQNLLRTQITNFTFNLGFS
  GKFYHTGTEEEDEGDDCLLGSVDEFWWFPHMWSHMQPHLFHNESSLIEQMILNKKFALEHGIPTDMGYAV
  SPHHSGVYPVHVQLYEAWKKVWNIKITSTEEYPHLKPARYRRGFIHKNIMVLPRQTCGLFTHTIFYKEYP
  GGPRELDKSIHGGELFFTVVLNPISIFMTHLSNYGNDRLGLYTFVNLANFVQTWTNLRLQTLPPAQLAHK
  YFELFPDQKDPLWQNPCDDKRHRDIWSKEKTCDRLPKFLVIGPQKTGTTALCLFLIMHPSILSNSPSPKS
  FEEVHGFLPSPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC079622
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC079622, AAH79622
• RefSeq Size: 5317 bp
• RefSeq ORF: 1928 bp
• Locus ID: 83398
• MW: 73.8 kDa