Ralbp1 (NM_009067) Mouse Tagged ORF Clone

CAT#: MR209748

  • TrueORF®

Ralbp1 (Myc-DDK-tagged) - Mouse ralA binding protein 1 (Ralbp1), transcript variant 1


  "NM_009067" in other vectors (4)


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Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ralbp1
Synonyms Rip1; RLIP76
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209748 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGAGTGCTTCCTGCCCCCGAGCAGCAGCCCTAGTGAACACCGCAGGGCCGAGCATGGCAGCGGGC
TGACCCGGACCCCCAGCTCTGAGGAGATCAGTCCTACAAAGTTCCCTGGATTGTACCGGACGGGCGAGCC
CTCGCCTCCCCATGACGTCCTCCATGAGCCTCCTGATACAGTGTCAGATGACGACAAAGACCATGGAAAG
AAAAAAGGGAAATTTAAGAAAAAGGAGAAAAGGACTGAAGGCTACGCTGCCTTCCAGGAAGACAGCTCTG
GAGATGAAGCTGAAAGTCCTTCCAAGGTGAAGAGGTCCAAGGGAATCCATGTGTTCAAAAAGCCCAGTTT
CTCTAAAAAGAAGGAAAAAGATTTTAAAATCAAAGAAAAACCCAAAGAAGAAAAGCATAAAGAAGAAAAG
CACAAAGAGGAAAAGCATAAAGAAAAGAAATCGAAAGACTTGACAGCAGCTGATGTTGTTAAACAGTGGA
AGGAAAAGAAGAAAAAGAAAAAGCCAATTCAGGAGCCAGAGGTGCCTCAGATGGACGCACCGAGTGTCAA
GCCCATTTTTGGGGTTCCCTTGGTTGACGCAGTAGAGAGGACCATGATGTACGACGGCGTCCGGCTGCCA
GCTGTCTTCCGGGAGTGTGTGGACTACATGGAGAAGCACGGCATGAAGTGTGAAGGCGTCTACCGGGTGT
CAGGAATCAAATCAAAGGTAGATGAGCTAAAAGCAGCCTATGACCGAGAGGAGTCTCCAAACTTAGAAGA
ATATGAGCCTAACACTGTAGCCAGCTTGCTGAAGCAGTATTTGCGAGACCTTCCAGAGAATTTGCTTACC
AAAGAGCTTATGCCCCGATTTGAAGAGGCTTGTGGGAAGACCACAGAGATGGAGAAAGTGCAGGAATTCC
AGCGCTTGCTCCGGGAACTGCCGGAGTGCAATCATCTTCTGCTTTCCTGGCTCATTGTGCACCTGGACCA
CGTCATTGCGAAGGAGCTGGAAACGAAGATGAACATCCAGAACATCTCTATAGTGCTGAGCCCCACCGTG
CAGATCAGCAATCGGGTCCTGTACGTGCTTTTCACACATGTGCAAGAGCTCTTTGGCACCGTGGTCCTGA
AGCAAGTAACAAGACCTCTGCGCTGGTCCAACATGGCCACGATGCCCACACTGCCAGAGACCCAAGCAGG
CATCAAGGAGGAGATCAGGAGACAGGAGTTCCTTTTGAATTGTTTACATCGAGATCTGCAGGGCGGGATA
AAGGACTTATCTAAAGAAGAAAGATTATGGGAAGTACAGAGGATTCTGACTGCCCTCAAGAGAAAGCTGA
GGGAAGCTAAAAGACAAGAGTGTGAAACCAAGATTGCACAGGAGATAGCTAGTCTTTCGAAAGAGGATGT
TTCCAAAGAAGAAATGAATGAAAACGAAGAAGTTATAAATATCCTTCTTGCCCAGGAGAATGAAATCCTG
ACAGAACAGGAGGAGCTCCTGGCCATGGAGCAGTTCCTGCGTCGTCAGATCGCCTCAGAGAAAGAAGAGA
TCGACCGCCTCCGAGCTGAAATTGCGGAGATCCAGAGCCGTCAGCAGCATGGCCGCAGTGAGACGGAGGA
GTACTCGTCCGACAGCGAGAGTGAGAGCGAGGACGAGGAGGAGCTGCAGCTCATCCTGGAGGACCTGCAG
CGGCAGAACGAGGAGTTAGAAATAAAGAACAATCACTTGAACCAAGCAGTCCATGAGGAGCGGGAGGCCA
TCATCGAGCTCCGAGTGCAGCTCCGGCTGCTCCAGATGCAGCGAGCCAAGTCTGAGCAGCAGCCGCAGGA
GGAGGAGGAGCCCGAGAGGCGAGGGGGCACAGGCCCACCGCCCTGTGACGGTGTCCTCGAGGTGCGGGTA
GCCAAGGAGCAGGCGAAGGCCTCGCCCAGCAAAGACCGGAAGGAGACGCCCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209748 protein sequence
Red=Cloning site Green=Tags(s)

MTECFLPPSSSPSEHRRAEHGSGLTRTPSSEEISPTKFPGLYRTGEPSPPHDVLHEPPDTVSDDDKDHGK
KKGKFKKKEKRTEGYAAFQEDSSGDEAESPSKVKRSKGIHVFKKPSFSKKKEKDFKIKEKPKEEKHKEEK
HKEEKHKEKKSKDLTAADVVKQWKEKKKKKKPIQEPEVPQMDAPSVKPIFGVPLVDAVERTMMYDGVRLP
AVFRECVDYMEKHGMKCEGVYRVSGIKSKVDELKAAYDREESPNLEEYEPNTVASLLKQYLRDLPENLLT
KELMPRFEEACGKTTEMEKVQEFQRLLRELPECNHLLLSWLIVHLDHVIAKELETKMNIQNISIVLSPTV
QISNRVLYVLFTHVQELFGTVVLKQVTRPLRWSNMATMPTLPETQAGIKEEIRRQEFLLNCLHRDLQGGI
KDLSKEERLWEVQRILTALKRKLREAKRQECETKIAQEIASLSKEDVSKEEMNENEEVINILLAQENEIL
TEQEELLAMEQFLRRQIASEKEEIDRLRAEIAEIQSRQQHGRSETEEYSSDSESESEDEEELQLILEDLQ
RQNEELEIKNNHLNQAVHEEREAIIELRVQLRLLQMQRAKSEQQPQEEEEPERRGGTGPPPCDGVLEVRV
AKEQAKASPSKDRKETPI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009067
ORF Size 1947 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009067.1, NM_009067.2, NM_009067.3, NM_009067.4, NM_009067.5, NP_033093.1
RefSeq Size 4112 bp
RefSeq ORF 1947 bp
Locus ID 19765
MW 75 kDa

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