Dars2 (NM_172644) Mouse Tagged ORF Clone

CAT#: MR209779

Dars2 (Myc-DDK-tagged) - Mouse aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dars2
• Synonyms: 5830468K18Rik; aspRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209779 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCTTGGTTTTTGGTTAAGTCGTCTATGCAGGGGTTTATCCAGACCCATCGGGAAGACCATGCGGC
  CAATCTGGGGTTCTCTTTCCAGAAATCTGGCGTTGAGTTCACAAAGAATTCCAGAATTCAGTAGCTTTGT
  TGCTCGGACCAACACATGTGGAGAGTTGCGTTCGTCCCACTTAGGCCAAGAAGTCACTCTGTGTGGATGG
  GTTCAGTACCGCAGGCAGAACACCTTCTTAGTCCTAAGAGATTGCCACGGCCTTGTTCAGATTCTCATTC
  CCCAGGATGAGTCAGCCGCATCTGTGAGGGGGATTTTGTGTGAAGCCCCTGTGGAGTCTGTGGTGAGAGT
  ATCTGGGACAGTAATCTCCCGTCCTCCAGGACAAGAGAATCCGAAAATGCCGACGGGTGAGATTGAAATC
  AAAGTTAAAACAGCTGAGCTCCTGAATGCCTGCAAAAAGCTGCCCTTTGAGATTAAAGACTTTGTGAAGA
  AAACAGAGGCGCTCCGTTTGCAGTATCGCTACTTAGATCTGCGCAGCTTCCAGATGCAGTACAACTTGCG
  GCTGAGGTCGCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCACGGGTTTGTGGATATAGAA
  ACCCCAACCTTGTTTAAGAGGACCCCTGGAGGTGCCAAGGAGTTTTTAGTACCATCCAGGGAACCTGGAA
  AGTTTTATTCCCTCCCTCAAAGTCCTCAGCAATTTAAGCAGCTTCTGATGGTTGGAGGTTTAGACAGGTA
  TTTTCAGGTCGCTCGGTGTTACCGAGATGAAGGCTCAAGACCGGATAGACAGCCAGAGTTCACTCAGATT
  GACATAGAGATGTCCTTTGTAGAGCAGACTGGCATTCAGCGTTTAGTTGAGGGTTTGCTGCAGTATTCCT
  GGCCTGGTGATAAAGACCCTCTAGTGACTCCTTTCCCTTCCATGACTTTTGCTGAGGCACTGGCCACCTA
  TGGAACTGATAAGCCAGATACTCGCTTTGGGATGAAGATTGTGGATGTCAGTGATGTGTTCAGAAACACA
  GAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGACG
  GAGCGAAATACTTAAGGAAGGAAGACATTGAGTTCATTAGGAAGTTTGCAGTTCACCATTTCAGTCAGGA
  AGTCCTACCTATATTCCTGAATGCCAAGAAGAACTGGAGTTCGCCATTTGCTAAGTTCATAATGGAGGAG
  GAAAGACTGGAATTGGCCAGATCGATGGAGATACAAGAAGAAGACATAGTGTTACTGACTGCTGGGGAAC
  ATGAGAAAGCATGCTCTCTGTTGGGAAAGTTACGGCTAGAGTGTGCAGACCTTTTGGAGATGAGAGGAGC
  GGTGCTCCGAGACCCAGCAGTGTTCTCTTTTCTTTGGGTGGTTGACTTTCCACTCTTCCTTGCCAAGGAA
  GAGAGTCCCACAGAGCTGGAGTCAGCCCATCACCCATTTACTGCTCCTAACTCCAGTGACATTCACCTCC
  TCTATACTGAGCCGGAAAAGGTTCGTGGCCAACACTATGACTTGGTTTTAAACGGCAATGAGATAGGAGG
  TGGTTCTGCCCGAATCCATGATGCACAGCTACAGAGGTACATCCTGGAAACACTGCTAAAGGAAGATGTG
  AAATTGCTTTCCCATCTGCTTCAGGCTTTAGACTATGGGGCACCCCCACACGGAGGAATTGCCTTAGGGT
  TAGACAGACTGGTATGTCTTGTCACGGGAGCACCCAGCATCAGAGATGTCATAGCCTTCCCTAAATCCTA
  CCGAGGACAGGACCTTATGAGCAATGCTCCAGACTCCGTGTCTCCAGAAGAGCTGAAGCCTTACCATATC
  CACGTCTTGTGGCCAGCAGACTCAGAAGAGGAGAGTGCATCCGCCACACCTTCCAAACACTTGAGCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209779 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYLGFWLSRLCRGLSRPIGKTMRPIWGSLSRNLALSSQRIPEFSSFVARTNTCGELRSSHLGQEVTLCGW
  VQYRRQNTFLVLRDCHGLVQILIPQDESAASVRGILCEAPVESVVRVSGTVISRPPGQENPKMPTGEIEI
  KVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIE
  TPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQI
  DIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNT
  ELRFLQDALAKPQGTVKAICVHDGAKYLRKEDIEFIRKFAVHHFSQEVLPIFLNAKKNWSSPFAKFIMEE
  ERLELARSMEIQEEDIVLLTAGEHEKACSLLGKLRLECADLLEMRGAVLRDPAVFSFLWVVDFPLFLAKE
  ESPTELESAHHPFTAPNSSDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSARIHDAQLQRYILETLLKEDV
  KLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGQDLMSNAPDSVSPEELKPYHI
  HVLWPADSEEESASATPSKHLSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172644
• ORF Size: 1962 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_172644.1, NM_172644.2, NM_172644.3, NM_172644.4, NP_766232.1
• RefSeq Size: 3581
• RefSeq ORF: 1962
• Locus ID: 226539
• MW: 74 kDa