Sema7a (NM_011352) Mouse Tagged ORF Clone

CAT#: MR209876

Sema7a (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sema7a
• Synonyms: 2900057C09Rik; CDw108; H-Sema-L; H-Sema K1; M-Sema-L; sema K1; Semal; sema L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209876 representing NM_011352
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCCTCCTCCTCCCGGACGTGCCGCCCCCAGCGCACCGCGCGCCCGCGTCCTCAGCCTGCCGGCTC
  GGTTCGGGCTCCCGCTGCGGCTGCGGCTTCTGCTGGTGTTCTGGGTGGCCGCCGCCTCCGCCCAAGGCCA
  CTCGAGGAGCGGACCCCGCATCTCCGCCGTCTGGAAAGGGCAGGACCATGTGGACTTTAGCCAGCCTGAG
  CCACACACCGTGCTTTTCCATGAGCCGGGCAGCTTCTCTGTCTGGGTGGGTGGACGTGGCAAGGTCTACC
  ACTTCAACTTCCCCGAGGGCAAGAATGCCTCTGTGCGCACGGTGAACATCGGCTCCACAAAGGGGTCCTG
  TCAGGACAAACAGGACTGTGGGAATTACATCACTCTTCTAGAAAGGCGGGGTAATGGGCTGCTGGTCTGT
  GGCACCAATGCCCGGAAGCCCAGCTGCTGGAACTTGGTGAATGACAGTGTGGTGATGTCACTTGGTGAGA
  TGAAAGGCTATGCCCCCTTCAGCCCGGATGAGAACTCCCTGGTTCTGTTTGAAGGAGATGAAGTGTACTC
  TACCATCCGGAAGCAGGAATACAACGGGAAGATCCCTCGGTTTCGACGCATTCGGGGCGAGAGTGAACTG
  TACACAAGTGATACAGTCATGCAGAACCCACAGTTCATCAAGGCCACCATTGTGCACCAAGACCAAGCCT
  ATGATGATAAGATCTACTACTTCTTCCGAGAAGACAACCCTGACAAGAACCCCGAGGCTCCTCTCAATGT
  GTCCCGAGTAGCCCAGTTGTGCAGGGGGGACCAGGGTGGTGAGAGTTCGTTGTCTGTCTCCAAGTGGAAC
  ACCTTCCTGAAAGCCATGTTGGTCTGCAGCGATGCAGCCACCAACAGGAACTTCAATCGGCTGCAAGATG
  TCTTCCTGCTCCCTGACCCCAGTGGCCAGTGGAGAGATACCAGGGTCTATGGCGTTTTCTCCAACCCCTG
  GAACTACTCAGCTGTCTGCGTGTATTCGCTTGGTGACATTGACAGAGTCTTCCGTACCTCATCGCTCAAA
  GGCTACCACATGGGCCTTCCCAACCCTCGACCTGGCATGTGCCTCCCAAAAAAGCAGCCCATACCCACAG
  AAACCTTCCAGGTAGCTGATAGTCACCCAGAGGTGGCTCAGAGGGTGGAACCTATGGGGCCACTGAAGAC
  ACCATTGTTCCATTCTAAGTACCATTACCAGAAAGTGGTCGTTCACCGCATGCAAGCCAGCAATGGAGAG
  ACCTTCCATGTGCTTTATCTAACCACAGACAGGGGCACCATTCACAAGGTGGTGGAATCAGGGGACCAGG
  ACCATAGCTTTGTCTTCAATATCATGGAGATCCAGCCCTTTCACCGTGCAGCGGCCATCCAGGCTATATC
  ATTGGATGCTGACCGGCGGAAGCTCTATGTGACCTCCCAGTGGGAAGTGAGCCAGGTACCCCTGGACATG
  TGTGAGGTCTACAGCGGGGGCTGCCATGGCTGCCTCATGTCCCGAGACCCCTACTGTGGCTGGGACCAGG
  ACCGCTGCGTGTCTATCTACAGCTCCCAACGATCAGTGCTGCAGTCCATTAATCCAGCGGAGCCACACAG
  AGAGTGTCCCAACCCTAAACCAGACGAGGCTCCATTGCAGAAGGTTTCCCTGGCTCGGAACTCTCGATAC
  TACCTGACCTGCCCCATGGAGTCCCGCCACGCCACTTACTTATGGCGCCATGAGGAGAATGTGGAACAGA
  GCTGTGAGCCAGGCCACCAAAGCCCTAGCTGCATCCTGTTCATTGAGAACCTCACGGCCCGTCAGTATGG
  CCACTACCGCTGCGAGGCCCAAGAGGGCTCCTACCTCCGTGAGGCTCAACACTGGGAGCTGCTGCCAGAG
  GACAGAGCACTGGCCGAACAACTAATGGGCCATGCCCGGGCCCTGGCCGCCTCCTTCTGGCTGGGGGTCC
  TGCCCACACTCATACTTGGTCTGCTGGTTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209876 representing NM_011352
  Red=Cloning site Green=Tags(s)
  
  MTPPPPGRAAPSAPRARVLSLPARFGLPLRLRLLLVFWVAAASAQGHSRSGPRISAVWKGQDHVDFSQPE
  PHTVLFHEPGSFSVWVGGRGKVYHFNFPEGKNASVRTVNIGSTKGSCQDKQDCGNYITLLERRGNGLLVC
  GTNARKPSCWNLVNDSVVMSLGEMKGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESEL
  YTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWN
  TFLKAMLVCSDAATNRNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDRVFRTSSLK
  GYHMGLPNPRPGMCLPKKQPIPTETFQVADSHPEVAQRVEPMGPLKTPLFHSKYHYQKVVVHRMQASNGE
  TFHVLYLTTDRGTIHKVVESGDQDHSFVFNIMEIQPFHRAAAIQAISLDADRRKLYVTSQWEVSQVPLDM
  CEVYSGGCHGCLMSRDPYCGWDQDRCVSIYSSQRSVLQSINPAEPHRECPNPKPDEAPLQKVSLARNSRY
  YLTCPMESRHATYLWRHEENVEQSCEPGHQSPSCILFIENLTARQYGHYRCEAQEGSYLREAQHWELLPE
  DRALAEQLMGHARALAASFWLGVLPTLILGLLVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011352
• ORF Size: 1992 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011352.1, NM_011352.2, NP_035482.1
• RefSeq Size: 3290 bp
• RefSeq ORF: 1995 bp
• Locus ID: 20361
• MW: 75.4 kDa