Hdac10 (NM_199198) Mouse Tagged ORF Clone

CAT#: MR209891

Hdac10 (Myc-DDK-tagged) - Mouse histone deacetylase 10 (Hdac10), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hdac10
• Synonyms: AW548891; Hd10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209891 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACAGCACTTGTGTACCACGAGGACATGACAGCCACTCGACTGCTCTGGGATGACCCCGAGTGCG
  AAATTGAGTGCCCAGAGCGCCTGACAGCTGCCCTGGATGGCCTGCGGCAGCGTGGCCTGGAAGAAAGGTG
  CCTGTGTTTGTCAGCTTGTGAGGCATCAGAGGAAGAGTTGGGCTTGGTGCACAGCCCGGAATATATAGCC
  CTGGTGCAGAAGACCCAGACCCTGGATAAAGAGGAGCTCCACGCACTGTCTAAGCAGTACGATGCTGTCT
  ACTTTCACCCGGACACCTTTCACTGTGCCCGGCTGGCAGCGGGGGCTGCACTGCAGCTGGTCGATGCTGT
  GCTAACAGGAGCTGTGCACAATGGGCTTGCCCTGGTGAGGCCTCCAGGGCACCACAGTCAGAGGGCAGCT
  GCCAATGGATTCTGTGTGTTCAACAATGTGGCTCTAGCAGCCAAACATGCCAAGCAGAAATATGGGCTGC
  AGAGGATTCTCATTGTCGACTGGGATGTCCACCATGGCCAGGGCATCCAGTATATCTTCAACGATGACCC
  CAGTGTCCTTTATTTCTCTTGGCACCGCTATGAGCATGGAAGCTTCTGGCCGTTTCTCCCAGAGTCTGAT
  GCAGATGCAGTTGGCCAAGGGCAGGGCCAAGGTTTCACTGTCAATTTGCCCTGGAACCAGGTTGGGATGG
  GAAATGCCGACTATTTGGCTGCCTTTCTGCATGTGCTGCTCCCACTGGCCTTCGAGTTTGATCCTGAGTT
  GGTGCTGGTGTCGGCTGGATTTGACTCTGCTATCGGGGACCCTGAGGGGCAGATGCAGGCCACCCCTGAG
  TGCTTTGCCCATCTCACACAGCTGCTGCAGGTGCTGGCTGGTGGCCGGATTTGTGCTGTGTTGGAGGGTG
  GCTACCACTTGGAGTCCCTAGCACAGTCAGTGTGCATGATGGTGCAGACACTGCTTGGTGACCCCACACC
  TCCCCTTCTTGGGCTCATGGTGCCATGTCAGAGTGCCCTGGAGTCCATCCAGAGTGTCCAGACAGCCCAG
  ACCCCTTACTGGACAAGCCTCCAGCAAAATGTGGCCCCAGTTCTGAGTTCCAGCACCCACTCTCCTGAAG
  AGAGATCTCTGCGTCTGCTTGGTGAGAGCCCCACATGCGCAGTAGCAGAAGATTCACTGAGCCCCCTCCT
  GGACCAACTGTGCCTCCGCCCTGCACCTCCAATCTGCACAGCGGTGGCCTCGACTGTACCAGGTGCTGCT
  CTGTGCTTACCTCCTGGAGTGCTCCATCAAGAAGGGTCAGTCTTGAGGGAGGAGACCGAAGCCTGGGCCA
  GGCTTCACAAATCCCGGTTCCAGGATGAGGATCTTGCCACACTGGGGAAGATTCTGTGTCTCTTAGATGG
  AATCATGGATGGGCAGATAAGAAATGCCATAGCAACCACAACTGCCCTCGCCACAGCAGCAACATTGGAT
  GTACTCATTCAGCGATGCCTAGCTCGCAGAGCTCAGAGGGTGCTCTGTGTGGCCCTGGGACAACTGGATC
  GACCCCTGGACCTTGCAGATGATGGGAGAATTCTGTGGCTCAACATCCGGGGCAAGGATGCAGCCATCCA
  GTCCATGTTCCACTTCTCCACTCCACTGCCACAGACAACTGGAGGGTTTCTGAGCCTCATTCTGGGTCTG
  GTACTGCCCTTGGCCTATGGTTTCCAGCCTGATATGGTGTTGATGGCACTGGGGCCTGCCCATGGCCTGC
  AGAACGCTCAAGCTGCTCTCTTGGCTGCAATGCTTCGGAGCCCAGTAGGGGGCCGAATTCTAGCTGTAGT
  GGAAGAGGAATCCATACGCCTGCTTGCAAGGAGCCTGGCGCAGGCATTGCATGGAGAAACACCTCCCAGT
  CTGGGTCCTTTCTCGAAGGCCACTCCAGAGGAGATCCAGGCTCTGATGTTTCTAAAAGCTCGGCTGGAGG
  CTCGCTGGAAATTGCTGCAGGTGGCTGCTCCTCCACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209891 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGTALVYHEDMTATRLLWDDPECEIECPERLTAALDGLRQRGLEERCLCLSACEASEEELGLVHSPEYIA
  LVQKTQTLDKEELHALSKQYDAVYFHPDTFHCARLAAGAALQLVDAVLTGAVHNGLALVRPPGHHSQRAA
  ANGFCVFNNVALAAKHAKQKYGLQRILIVDWDVHHGQGIQYIFNDDPSVLYFSWHRYEHGSFWPFLPESD
  ADAVGQGQGQGFTVNLPWNQVGMGNADYLAAFLHVLLPLAFEFDPELVLVSAGFDSAIGDPEGQMQATPE
  CFAHLTQLLQVLAGGRICAVLEGGYHLESLAQSVCMMVQTLLGDPTPPLLGLMVPCQSALESIQSVQTAQ
  TPYWTSLQQNVAPVLSSSTHSPEERSLRLLGESPTCAVAEDSLSPLLDQLCLRPAPPICTAVASTVPGAA
  LCLPPGVLHQEGSVLREETEAWARLHKSRFQDEDLATLGKILCLLDGIMDGQIRNAIATTTALATAATLD
  VLIQRCLARRAQRVLCVALGQLDRPLDLADDGRILWLNIRGKDAAIQSMFHFSTPLPQTTGGFLSLILGL
  VLPLAYGFQPDMVLMALGPAHGLQNAQAALLAAMLRSPVGGRILAVVEEESIRLLARSLAQALHGETPPS
  LGPFSKATPEEIQALMFLKARLEARWKLLQVAAPPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199198
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199198.1, NM_199198.2, NP_954668.1
• RefSeq Size: 2424 bp
• RefSeq ORF: 2001 bp
• Locus ID: 170787
• MW: 72.1 kDa