Zc3hav1 (BC029090) Mouse Tagged ORF Clone

CAT#: MR209944

Zc3hav1 (Myc-DDK-tagged) - Mouse zinc finger CCCH type, antiviral 1 (cDNA clone MGC:27750 IMAGE:2651558)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zc3hav1
• Synonyms: 1200014N16, 2900058M19, ZAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209944 representing BC029090
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGATCCCGAGGTATTCTGTTTCATCACCAAGATCCTGTGCGCTCACGGGGGCCGCATGACCCTGG
  AGGAACTGCTGGGTGAGATCAGCCTCCCCGAAGCGCAACTCTACGAGCTGCTGAAGGCAGCGGGGCCCGA
  TCGCTTTGTGCTATTGGAGACTGGAGACCAGGCCGGGATCACTCGGTCGGTGGTGGCTACTACTCGAGCC
  CGCGTCTGCCGTCGCAAGTACTGCCAGAGACCCTGCGACAGCCTGCACCTTTGCAAGCTTAATCTGCTCG
  GCCGGTGCCACTATGCACAGTCCCAGCGGAACCTCTGCAAATATTCTCACGATGTTCTCTCGGAACAGAA
  CTTCCAGGTCCTGAAGAATCATGAGCTCTCCGGGCTTAACCAAGAGGAGCTGGCGGTCCTCCTGGTCCAA
  AGCGACCCTTTCTTCATGCCTGAGATATGCAAGAGTTACAAAGGAGAGGGCCGCAAACAGATCTGCGGGC
  AGCCGCAGCCCTGCGAGAGACTCCACATCTGTGAGCACTTCACCCGGGGCAACTGCAGTTACCTCAACTG
  TCTCAGGTCTCATAACCTGATGGACAGGAAGGTGTTGGCCATCATGAGGGAGCATGGGCTGAGTTCTGAC
  GTGGTCCAGAACATCCAGGACATCTGTAACAACAAACACACTCGGAGGAACCCCCCTAGCATGAGAGCTC
  CCCACCCACATCGCAGAGGCGGGGCACACAGGGACAGAAGCAAAAGCAGAGACCGCTTCCATCACAACAG
  TCTAGAGGTTCTCTCAACGGTCTCACCTCTGGGGTCTGGTCCCCCTAGCCCAGATGTCACCGGCTGTAAG
  GATCCCCTGGAGGATGTGTCTGCAGATGTCACCCAGAAGTTCAAGTACCTGGGGACTCAGGACCGTGCAC
  AGCTTTCCTCCGTCTCATCTAAGGCCGCTGGTGTCCGAGGACCCAGTCAAATGAGAGCAAGCCAGGAGTT
  TTTGGAGGATGGGGATCCAGATGGCTTGTTTTCTAGGAATCGTTCTGATTCGTCCACAAGTCGAACCTCT
  GCTGCTGGCTTTCCTCTCGTTGCGGCACAAAGAAATGAAGCTGGGGCCATGAAAATGGGCATGCCTTCAG
  GACACCACGTCGAGGTCAAGGGCAAGAACAAGGACATTGATCGCGTCCCGTTTTTAAATAGTTATATTGA
  TGGGGTAACAATGGAAGAAGCAACAGTTTCAGGAATTCTAGGTAAAAGGGCCACAGACAACGGTCTGGAA
  GAAATGATACTATCTAGCAACCATCAGAAGAGTGTGGCTAAGACCCAGGATCCCCAGACCACTGGCAGAA
  TCACTGACAGTGGCCAAGACACGGCATTCCTGCATAGTAAATATGAAGAAAACCCAGCGTGGCCAGATTC
  CCTATGCTACATCGTAAATCCTGTATCTCCTAGGATGGATGATCATGGCCTGAAGGAAATCTGTCTGGAT
  CATCTGTACAGGGGCTGTCAGCAGGTCAACTGCAACAAGAACCACTTCCATCTGCCCTACCGGTGGCAGC
  TGTTCATATTGCCCACTTGGATGGACTTTCAGGACATGGAGTATATCGAGCGGGCCTATTGTGATCCCCA
  AATTGAAATCATTGTGATAGAAAAACATCGGATCAATTTCAAGAAAATGACTTGTGATTCCTACCCCATC
  CGTCGCCTCTCCACTCCTTCATTTGTCGAAAAAACACTTAATTCTGTCTTCACCACCAAGTGGCTTTGGT
  ATTGGAGGAATGAATTGAATGAATATACTCAGTATGGGCATGAGAGCCCAGGCCATACCAGCTCCGAAAT
  TAATTCTGCATACCTGGAGTCTTTCTTCCACTCCTGTCCCAGGGGAGTTTTGCAGTTCCACGCTGGTTCA
  CAGAATTACGAGTTAAGCTTTCAAGGGATGATTCAGACGAATATAGCTTCCAAGACTCAAAGGCATGTTG
  TGAGAAGGCCAGTTTTTGTTTCTTCGAAGGATGTGGAGCAGAAGAGAAGAGGTCCAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209944 representing BC029090
  Red=Cloning site Green=Tags(s)
  
  MTDPEVFCFITKILCAHGGRMTLEELLGEISLPEAQLYELLKAAGPDRFVLLETGDQAGITRSVVATTRA
  RVCRRKYCQRPCDSLHLCKLNLLGRCHYAQSQRNLCKYSHDVLSEQNFQVLKNHELSGLNQEELAVLLVQ
  SDPFFMPEICKSYKGEGRKQICGQPQPCERLHICEHFTRGNCSYLNCLRSHNLMDRKVLAIMREHGLSSD
  VVQNIQDICNNKHTRRNPPSMRAPHPHRRGGAHRDRSKSRDRFHHNSLEVLSTVSPLGSGPPSPDVTGCK
  DPLEDVSADVTQKFKYLGTQDRAQLSSVSSKAAGVRGPSQMRASQEFLEDGDPDGLFSRNRSDSSTSRTS
  AAGFPLVAAQRNEAGAMKMGMPSGHHVEVKGKNKDIDRVPFLNSYIDGVTMEEATVSGILGKRATDNGLE
  EMILSSNHQKSVAKTQDPQTTGRITDSGQDTAFLHSKYEENPAWPDSLCYIVNPVSPRMDDHGLKEICLD
  HLYRGCQQVNCNKNHFHLPYRWQLFILPTWMDFQDMEYIERAYCDPQIEIIVIEKHRINFKKMTCDSYPI
  RRLSTPSFVEKTLNSVFTTKWLWYWRNELNEYTQYGHESPGHTSSEINSAYLESFFHSCPRGVLQFHAGS
  QNYELSFQGMIQTNIASKTQRHVVRRPVFVSSKDVEQKRRGPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC029090
• ORF Size: 2019 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC029090, AAH29090
• RefSeq Size: 2980 bp
• RefSeq ORF: 2021 bp
• Locus ID: 78781
• MW: 109.2 kDa