C1r (BC004637) Mouse Tagged ORF Clone

CAT#: MR210176

C1r (Myc-DDK-tagged) - Mouse complement component 1, r subcomponent (cDNA clone MGC:6404 IMAGE:3586055)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1r
• Synonyms: C1rb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210176 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTCTTTGCCCTCCTGGTGACCCTGTTCTATGGGGTGGAAGGCTCCATTTACCTCCCTCAGAAGC
  TCTATGGAGAGGTGACCTCCCCTCTGTATCCCAAGCCTTACCCCAGTGACTTGGAGACAACCACTGTGAT
  CACTGTCCCCATGGGGTACAGGGTGAAGCTGGTCTTCTGGCAGTTTGACGTGGAGCCTTCTGAAGGCTGC
  TTCTATGACTATGTTAAGATTTCTGCTGATAAGCAAACACTGGGGAGGTTCTGTGGGCAGCTGGATTCTC
  CCCTGGGCAACCCCCCAGGAAGCAAGGAATTCATGTCCCAAGGAAACAAGATGCTGCTGACCTTCCACAC
  AGACTTCTCCAATGAGGAGAATGGGACCATCATGTTCTACAAGGGCTTCCTGGCCTACTACCAGGCTGTA
  GACCTTGATGAATGTGCATCGCAGCCCAACTCAGTGGAAGAGGGTTTGCAGCCCCGATGCCAACATCTGT
  GTCACAACTATGTTGGAGGCTACTTCTGTTCCTGCCATCCTGGCTATGAGCTTCAGAAAGATGGGCAATC
  CTGCCAGGCTGAGTGCAGCAGTGAGCTCTACACAGAGCCCTCAGGCTATGTCTCCAGCCTTGAATACCCT
  CAGCCCTATCCACCGGATCTACGCTGCAACTACAGCATCCGGGTGGAGAGGGGCCTCACTGTGCACCTCA
  AGTTCCTGGATCCCTTTGAAATTGATGACCACCAGCAAGTGCACTGCCCCTATGACCAGCTCCAGATCTA
  CGCTAATGGGAAGAACTTGGGTGAATTCTGTGGAAAGCAAAGGCCTCCAGACCTTGACACCAGCAGCAAT
  GCAGTGGATCTGCTGTTCTTCACAGATGAGTCAGGGGACAGCCGAGGCTGGAAGCTGCACTACACCACTG
  AAACCATCAAGTGCCCCCAGCCCAAGGCTCTGGATGAGTTCACCATCATCCAGGATCCGCAGCCTCAGTA
  CCAGTTCCGGGATTACTTCATTGTCACCTGCAAGCAAGGCTACCAGCTCATGGAGGGAAATCAGGCGCTA
  CTCTCCTTCACAGCTGTTTGCCAGCATGATGGCACATGGCATCGTGCCATGCCCAGGTGCAAGATCAAGA
  ACTGTGGGCAGCCCCAAAGCCTGTCTAATGGGGACTTCCGCTACATCACCACAAAAGGCGTGACCACCTA
  TGAAGCCAGTATCCAGTATCACTGCCATGAACCATATTACAAGATGCTGACCAGAGCTGGCAGCAGCGAG
  TCCATGCGAGGGATATATACCTGCACAGCCCAAGGCATTTGGAAGAATGAAGAGGAAGGAGAGAAAATGC
  CCCGGTGTCTGCCAGTGTGTGGGAAACCTGTCAACCCTGTGACACAGAAGGAGCGCATCATCAGAGGGCA
  GCCAGCCAGGCCCGGCAACTTCCCCTGGCAGGCCTTCACCACTACCCACGGGCGAGGGGGTGGGGCCCTG
  CTTGGAGACCGCTGGATCCTCACAGCAGCCCACACCATCTACCCCAAGCATCACAACAAGGAAAACGACA
  ATGCCAACCCCAAAATGCTTGTTTTCCTGGGCCACACAAATGTGGAACAGATCAAAAAACTGGGACATCA
  CCCAGTCCGTAGGGTCATCATACACCCAGACTACCGCCAAGATGAACCTAACAATTTTGAAGGAGACATT
  GCTCTACTGGAGCTGGAAAACAGTGTCACACTGGGCCCCGAACTCCTCCCCATCTGTCTCCCAGACAATG
  AGACCTTCTATGGCCAAGGCCTCATGGGTTATGTCAGCGGATTCGGGATAACAGAAGATAAGTTAGCTTT
  CGATCTCAGGTTCGTCAGACTGCCTGTAGCTGACAGTGAGGCATGCCAGAGATGGCTCCAGACAAAAAAG
  GATACTTCTCCATTTTCTCAAAATATGTTCTGTTCTGGGGACCCAGCTGTACAGCAAGACGCCTGCCAAG
  GGGACAGTGGGGGTGTTTTTGCAGTCAGGGACAGAAATCGTGATATCTGGGTGGCTACGGGCATCGTATC
  CTGGGGCATTGGGTGTGGTGAGGGATATGGCTTCTACACCAAGGTACTGAATTATGTTGACTGGATCAAG
  AAAGAGATGGGAGACGAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210176 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWLFALLVTLFYGVEGSIYLPQKLYGEVTSPLYPKPYPSDLETTTVITVPMGYRVKLVFWQFDVEPSEGC
  FYDYVKISADKQTLGRFCGQLDSPLGNPPGSKEFMSQGNKMLLTFHTDFSNEENGTIMFYKGFLAYYQAV
  DLDECASQPNSVEEGLQPRCQHLCHNYVGGYFCSCHPGYELQKDGQSCQAECSSELYTEPSGYVSSLEYP
  QPYPPDLRCNYSIRVERGLTVHLKFLDPFEIDDHQQVHCPYDQLQIYANGKNLGEFCGKQRPPDLDTSSN
  AVDLLFFTDESGDSRGWKLHYTTETIKCPQPKALDEFTIIQDPQPQYQFRDYFIVTCKQGYQLMEGNQAL
  LSFTAVCQHDGTWHRAMPRCKIKNCGQPQSLSNGDFRYITTKGVTTYEASIQYHCHEPYYKMLTRAGSSE
  SMRGIYTCTAQGIWKNEEEGEKMPRCLPVCGKPVNPVTQKERIIRGQPARPGNFPWQAFTTTHGRGGGAL
  LGDRWILTAAHTIYPKHHNKENDNANPKMLVFLGHTNVEQIKKLGHHPVRRVIIHPDYRQDEPNNFEGDI
  ALLELENSVTLGPELLPICLPDNETFYGQGLMGYVSGFGITEDKLAFDLRFVRLPVADSEACQRWLQTKK
  DTSPFSQNMFCSGDPAVQQDACQGDSGGVFAVRDRNRDIWVATGIVSWGIGCGEGYGFYTKVLNYVDWIK
  KEMGDEN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC004637
• ORF Size: 2121 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004637, AAH04637
• RefSeq Size: 2409 bp
• RefSeq ORF: 2123 bp
• Locus ID: 50909
• MW: 80.1 kDa
• Gene Summary: C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. [UniProtKB/Swiss-Prot Function]