Capn1 (NM_007600) Mouse Tagged ORF Clone

CAT#: MR210215

Capn1 (Myc-DDK-tagged) - Mouse calpain 1 (Capn1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Capn1
• Synonyms: Capa-1; Capa1; mu-calpin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210215 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGAGGAGTTAATCACCCCTGTGTACTGCACCGGGGTGTCAGCCCAAGTACAGAAGAAGCGGGACA
  AGGAGCTGGGCTTGGGCCGCCATGAAAACGCCATCAAGTACCTGGGCCAGGATTATGAAACGCTTCGGGC
  AAGATGCCTGCAGAGTGGGGTCCTCTTCCAAGACGAGGCCTTCCCTCCGGTTTCTCATAGCCTGGGCTTC
  AAGGAACTGGGTCCTCATTCCTCTAAAACCTATGGCATCAAATGGAAGCGGCCTACGGAACTGATGTCAA
  ACCCCCAGTTCATCGTGGATGGAGCCACCCGGACAGACATCTGCCAGGGAGCACTGGGGGACTGTTGGCT
  CCTGGCTGCCATTGCCTCCCTCACCCTCAACGAGACTATTCTGCACCGAGTGGTTCCCTACGGCCAGAGC
  TTTCAGGATGGCTACGCTGGCATCTTTCATTTCCAGCTGTGGCAGTTCGGGGAGTGGGTCGACGTGGTCA
  TAGATGACTTGCTGCCCACCAAGGACGGGAAGCTGGTGTTCGTGCACTCTGCCCAGGGCAACGAGTTCTG
  GAGCGCACTGCTGGAGAAAGCCTATGCTAAAGTGAATGGCAGCTATGAGGCTCTTTCGGGAGGCTGCACC
  TCAGAGGCCTTCGAGGACTTTACCGGTGGGGTCACTGAGTGGTACGACCTGCAGAAGGCCCCCAGCGACC
  TCTACCAGATCATTCTAAAGGCCCTGGAACGAGGCTCCTTGCTGGGCTGCTCCATTAATATCTCCGATAT
  CCGTGATTTAGAGGCTATTACTTTTAAGAACCTGGTGAGGGGCCATGCGTACTCTGTGACGGGCGCCAAG
  CAGGTAACTTACCAGGGCCAGCGGGTGAACCTAATCCGGATGCGGAACCCCTGGGGTGAAGTGGAGTGGA
  AAGGACCCTGGAGTGACAGCTCCTATGAGTGGAACAAAGTGGACCCCTATGAACGAGAGCAGCTGAGGGT
  CAAGATGGAGGATGGGGAGTTCTGGATGTCGTTCCGAGACTTCATCCGTGAATTCACCAAACTGGAAATC
  TGCAACCTTACACCGGACGCCCTTAAGAGCAGGACCCTCCGGAATTGGAATACCACATTTTACGAGGGCA
  CCTGGCGTCGGGGAAGCACCGCTGGAGGCTGCAGGAACTACCCAGCTACCTTCTGGGTAAACCCCCAGTT
  CAAGATCCGGTTGGAGGAGGTGGATGACGCAGACGACTATGACAACCGGGAGTCGGGCTGCAGCTTCTTG
  CTGGCCCTCATGCAGAAACACCGCCGCAGGGAGCGTCGCTTTGGCCGGGACATGGAGACCATCGGTTTTG
  CAGTGTACCAGGTCCCTCGGGAGCTGGCGGGTCAGCCTGTGCACTTGAAGCGTGATTTCTTCCTGGCCAA
  CGCTTCTCGGGCGCAGTCAGAGCACTTCATCAACCTTCGGGAAGTCAGCAACCGTATCCGCCTGCCGCCC
  GGGGAGTACATAGTGGTGCCCTCCACCTTCGAGCCCAACAAAGAAGGCGACTTCCTGCTGCGCTTCTTCT
  CAGAGAAGAAGGCTGGGACCCAGGAACTAGATGACCAGATCCAGGCCAACCTCCCTGATGAGAAAGTTCT
  CTCTGAAGAGGAGATTGATGACAACTTCAAGACCTTGTTCAGCAAGTTGGCAGGGGATGACATGGAGATC
  AGCGTCAAGGAGCTACAGACCATCTTGAACAGGATCATTAGCAAACACAAAGACCTGCGCACTAATGGCT
  TCAGCCTGGAGTCGTGCCGCAGCATGGTGAACCTCATGGATCGAGATGGCAACGGGAAGCTGGGTCTGGT
  GGAGTTCAACATCCTGTGGAACCGCATCCGAAATTACCTGACCATCTTCCGGAAGTTTGACCTGGACAAG
  TCTGGCAGCATGAGTGCCTATGAGATGAGGATGGCCATCGAGGCTGCAGGCTTCAAGCTTAACAAGAAGC
  TGCATGAACTCATAATCACCCGCTACTCGGAGCCCGACCTGGCCGTGGACTTTGACAACTTTGTGTGCTG
  TCTTGTGCGGCTGGAGACCATGTTCCGGTTTTTCAAACTTCTGGACACAGACCTGGATGGTGTTGTGACC
  TTTGATCTATTTAAGTGGCTCCAGCTGACTATGTTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210215 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTEELITPVYCTGVSAQVQKKRDKELGLGRHENAIKYLGQDYETLRARCLQSGVLFQDEAFPPVSHSLGF
  KELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQS
  FQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCT
  SEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAK
  QVTYQGQRVNLIRMRNPWGEVEWKGPWSDSSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEI
  CNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFL
  LALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPP
  GEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEI
  SVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDK
  SGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKLLDTDLDGVVT
  FDLFKWLQLTMFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007600
• ORF Size: 2142 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007600.1, NM_007600.2, NM_007600.3, NP_031626.1
• RefSeq Size: 3119 bp
• RefSeq ORF: 2142 bp
• Locus ID: 12333
• MW: 82.1 kDa
• Gene Summary: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. [UniProtKB/Swiss-Prot Function]