Clcn4 (BC005553) Mouse Tagged ORF Clone

CAT#: MR210240

Clcn4 (Myc-DDK-tagged) - Mouse chloride channel 4-2 (cDNA clone MGC:6024 IMAGE:3586154)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clcn4
• Synonyms: Clc4-2|Clcn4-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210240 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTCCTCGAGGAGCCCTTCCCTGACGTGGGGACCTACGAGGACTTCCACACCATAGACTGGCTGA
  GGGAAAAGTCCCGGGATACCGACAGACATAGGAAGATCACCAGCAAAAGTAAGGAGTCTATTTGGGAGTT
  CATCAAGAGCCTGCTGGACGCGTGGTCGGGATGGGTGGTGATGCTACTCATTGGGCTGCTGGCAGGTACC
  TTGGCTGGAGTCATCGATCTCGCTGTGGATTGGATGACGGACCTCAAGGAGGGGGTCTGTCTGTCCGCAT
  TCTGGTACAGCCATGAACAGTGCTGTTGGACCTCCAACGAGACCACTTTTGAGGACAGGGACAAGTGTCC
  CCTGTGGCAGAAGTGGTCAGAGCTTCTTCTGAGCCAGTCAGAGGGCGCCAGCGCTTACATTCTGAATTAC
  TTAATGTACATTCTATGGGCGTTGCTGTTTGCATTTCTGGCTGTCTCCCTGGTACGTGTGTTCGCACCGT
  ATGCCTGTGGCTCTGGCATACCCGAGATAAAGACTATTTTGAGTGGCTTTATCATCAGGGGCTACTTGGG
  GAAATGGACTCTTCTAATCAAGACTGTCACCCTCGTGCTCGTCGTATCCTCTGGCCTGAGCCTTGGCAAA
  GAGGGCCCACTGGTGCATGTGGCATGTTGCTGTGGCAACTTCTTCAGCAGCCTTTTCTCCAAGTATAGCA
  AGAATGAAGGCAAGAGGCGTGAGGTGCTTTCAGCTGCAGCTGCTGCTGGTGTCTCTGTGGCCTTTGGTGC
  TCCGATAGGAGGTGTGCTCTTCAGTCTAGAGGAGGTCAGTTACTACTTTCCCTTGAAAACCTTGTGGAGG
  TCATTCTTTGCAGCCCTGGTGGCTGCCTTCACACTGCGCTCCATCAACCCCTTTGGAAATAGCCGCCTGG
  TTCTCTTTTACGTGGAGTATCATACACCCTGGAGGCGTAAGACCACCAGGCTGGGCAGGTACCCAGTGTT
  GGAGGTTATTGCGGTGACAGCCGTCACCGCCATCGTGGCCTACCCCAATCCCTACACTCGCCAGAGCACC
  AGTGAGCTCATCTCTGAGCTCTTCAACGATTGTGGGGCTCTCGAGTCTTCTCAGCTCTGTGACTACATCA
  ACGACCCCAACATGACTCGGCCTGTGGATGACATTCCGGACCGGCCGGCTGGGGTTGGAGTTTACACAGC
  CATGTGGCAGCTGGCCTTGGCACTGATCTTCAAAATAGTCATTACTATATTTACCTTTGGCATGAAGATT
  CCCTCAGGTCTCTTCATCCCCAGTATGGCTGTCGGAGCCATGGCAGGCCGGATGGTGGGAATCGGTGTGG
  AGCAGCTGGCCTACCATCACCATGACTGGATCATCTTCAGGAACTGGTGCAGGCCTGGAGCGGACTGTGT
  CACACCAGGGCTTTATGCGATGGTGGGAGCTGCAGCCTGTCTAGGTGGGGTGACTAGGATGACAGTGTCT
  CTAGTGGTCATTATGTTTGAACTGACTGGAGGTCTGGAGTATATTGTACCCCTGATGGCAGCTGCTGTCA
  CCAGCAAGTGGGTGGCTGATGCCTTTGGGAAAGAAGGGATTTATGAAGCCCACATCCATCTGAATGGGTA
  CCCATTTCTTGATGTGAAGGATGAGTTCACCCACCGTACGCTGGCCACTGATGTGATGCGGCCCCGGAGG
  GGAGAACCGCCATTATCGGTACTAACCCAGGACAGCATGACTGTGGAGGACGTGGAGACTCTCATCAAGG
  AGACAGACTACAACGGCTTTCCTGTGCTCGTCTCCAGAGACTCGGAGCGTCTCATCGGGTTTGCCCAGAG
  GCGGGAGCTAATCTTGGCTATAAAAAATGCCAGGCAGAGGCAAGAGGGCATTGTGAGCAATTCCATCATG
  TACTTCACAGAGGAGCCTCCTGAGCTGCCTGCCAACAGCCCACATCCACTGAAGCTGAGGCGCATTTTGA
  ACCTGAGCCCTTTCACGGTCACAGATCACACCCCCATGGAGACGGTGGTGGACATTTTCCGGAAACTGGG
  GCTCCGACAATGCCTGGTGACACGGAGTGGGAGACTTCTTGGGATCATCACAAAAAAGGATGTTCTGAGA
  CACATGGCCCAGATGGCAAACCAGGACCCTGAATCCATCATGTTTAAT
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210240 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDFLEEPFPDVGTYEDFHTIDWLREKSRDTDRHRKITSKSKESIWEFIKSLLDAWSGWVVMLLIGLLAGT
  LAGVIDLAVDWMTDLKEGVCLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLLSQSEGASAYILNY
  LMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGK
  EGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWR
  SFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWRRKTTRLGRYPVLEVIAVTAVTAIVAYPNPYTRQST
  SELISELFNDCGALESSQLCDYINDPNMTRPVDDIPDRPAGVGVYTAMWQLALALIFKIVITIFTFGMKI
  PSGLFIPSMAVGAMAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVS
  LVVIMFELTGGLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRR
  GEPPLSVLTQDSMTVEDVETLIKETDYNGFPVLVSRDSERLIGFAQRRELILAIKNARQRQEGIVSNSIM
  YFTEEPPELPANSPHPLKLRRILNLSPFTVTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLR
  HMAQMANQDPESIMFN
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005553
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005553, AAH05553
• RefSeq Size: 2447 bp
• RefSeq ORF: 2150 bp
• Locus ID: 12727
• MW: 80.2 kDa