Mcm7 (NM_008568) Mouse Tagged ORF Clone

CAT#: MR210265L4

Lenti ORF clone of Mcm7 (mGFP-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: mGFP
• Symbol: Mcm7
• Synonyms: AI747533; mCDC47; Mcmd7
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(MR210265).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_008568
• ORF Size: 2160 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008568.2, NP_032594.1
• RefSeq Size: 2396
• RefSeq ORF: 2160
• Locus ID: 17220
• Gene Summary: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProtKB/Swiss-Prot Function]