Mcm7 (NM_008568) Mouse Tagged ORF Clone

CAT#: MR210265

Mcm7 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mcm7
• Synonyms: AI747533; mCDC47; Mcmd7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210265 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTTAAGGACTACGCGATCGAAAAAGAAAAAGTTAAGAAGTTCCTCCAAGAGTTTTATTACGAGA
  ATGAGCTTGGAAAGAAGCAGTTCAAGTATGGGACCCAGTTGGTTCATCTGGCTCATCGGGAACAAGTGGC
  ACTGTATGTGGACCTGGATGACATAGCTGAAGATGACCCTGAGTTGGTCGACTCAATTTGCGAGAATGCC
  AAGCGCTACTCAAGACTGTTTGGTGATGTTGTGCAAGAGCTTTTGCCTGAGTACAAGGAGAAGGAGGTGG
  TGAATAAGGATGTCTTAGATGTTTACATTGAACACCGGCTGATGATGGAGCAGCGAAGCAGAGACCCTGG
  GGCAGTCCGGAACCCCCAAAACCAGTATCCTTCTGAACTCATGCGAAGATTTGAGTTGTATTTCCGAGGC
  CCAAGTAGCAGCAAGCCTCGAGTGATCCGGGAAGTACGAGCTGACTCTGTGGGGAAATTGTTAACTGTGC
  GTGGCATTGTCACTCGTGTGTCTGAAGTCAAGCCCAGGATGGTGGTGGCCACATACACTTGTGATCAGTG
  TGGGGCAGAGACCTACCAGCCAATCCAGTCTCCCACTTTCATGCCTCTGATCATGTGCCCCAGCCAGGAG
  TGCCAGACCAATCGCTCAGGGGGGCGGCTGTACCTACAGACTCGTGGCTCCAAATTTGTTAAATTCCAGG
  AAATGAAGATTCAAGAGCATAGTGACCAAGTTCCTGTGGGAAACATCCCTCGTAGCATCACAGTGGTTCT
  AGAAGGTGAGAACACAAGAATTGCCCAGCCTGGTGACCATGTCAGCGTCACTGGCATCTTCTTGCCAGTC
  CTGCGCACAGGGTTCCAACAGATGGCACAGGGCTTACTCTCAGAAACTTACCTGGAAGCCCACTGGATTG
  TGAAGATGACAAAGAGTGACGATGATGTCTCTGGGGCCGGAGAGCTGAGCTCAGAGGAGCTGAAGCAGAT
  TGCAGAGGAGGATTTCTATGAGAAGCTGGCCGCTTCTATTGCTCCGGAGATCTATGGACATGAAGATGTG
  AAGAAGGCGCTGCTGCTGCTTCTGGTGGGTGGTGTGGACCAGTCTCCTCAAGGCATGAAGATCAGAGGAA
  ACATCCACATCTGCCTTATGGGAGACCCTGGTGTGGCCAAATCTCAGCTCCTATCTTACATCGACCGTCT
  GGCACCGCGAAGTCAGTACACAACAGGCCGTGGCTCCTCTGGTGTAGGGCTCACAGCAGCTGTGTTGAGA
  GACTCTGTGAGTGGAGAACTGACTTTAGAGGGCGGTGCCCTGGTGCTGGCTGACCAGGGCGTGTGCTGCA
  TTGATGAGTTTGACAAGATGGCCGAGGCTGACCGCACAGCCATCCATGAGGTTATGGAGCAGCAGACCAT
  CTCCATCGCCAAGGCGGGGATTCTCACCACGCTCAATGCCCGATGCTCCATCCTGGCTGCGGCCAATCCT
  GCCTACGGCCGATATAATCCCCGCCGCAGTCTGGAGCAGAACGTCCAGCTTCCGGCTGCTCTGCTTTCTC
  GATTCGACCTCCTCTGGCTGATTCAAGACCGGCCCGACAGAGACAATGACCTCCGGTTGGCCCAGCACAT
  CACCTATGTCCACCAGCACAGCCGGCAGCCCCCTGCCCAATTTGAACCTTTGGACATGAAACTTATGAGA
  CGGTACATAGCCATGTGCCACGAGAGACAGCCCACAGTGCCTGAATCTCTGGCTGACTACATCACCGCAG
  CATATGTGGAGATGAGGCGAGAGGCCCGGGCCAGTAAGGATGCCACCTATACTTCTGCCCGGACCCTGCT
  GGCCATTCTCCGACTTTCCACTGCTCTGGCTCGACTTCGGATGGTAGACATTGTGGAGAAGGAAGATGTA
  AATGAAGCCATAAGGTTGATGGAGATGTCGAAGGACTCCCTTCTAGGTGAAAAGGGACAGACAGCTAGGA
  CCCAGAGGCCAGCAGATGTGATATTTGCCACCATCCGTGAATTGGTGTCACGGGGGCGGAGTGTCCACTT
  TTCTGAGGCTGAACAGCGCTGCATATCTCGTGGCTTCACACCAGCCCAGTTCCAGGCAGCTCTGGATGAA
  TATGAGGAGCTAAATGTCTGGCAGGTTAATACTTCTCGGACACGGATCACCTTTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210265 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALKDYAIEKEKVKKFLQEFYYENELGKKQFKYGTQLVHLAHREQVALYVDLDDIAEDDPELVDSICENA
  KRYSRLFGDVVQELLPEYKEKEVVNKDVLDVYIEHRLMMEQRSRDPGAVRNPQNQYPSELMRRFELYFRG
  PSSSKPRVIREVRADSVGKLLTVRGIVTRVSEVKPRMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQE
  CQTNRSGGRLYLQTRGSKFVKFQEMKIQEHSDQVPVGNIPRSITVVLEGENTRIAQPGDHVSVTGIFLPV
  LRTGFQQMAQGLLSETYLEAHWIVKMTKSDDDVSGAGELSSEELKQIAEEDFYEKLAASIAPEIYGHEDV
  KKALLLLLVGGVDQSPQGMKIRGNIHICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLR
  DSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNARCSILAAANP
  AYGRYNPRRSLEQNVQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPAQFEPLDMKLMR
  RYIAMCHERQPTVPESLADYITAAYVEMRREARASKDATYTSARTLLAILRLSTALARLRMVDIVEKEDV
  NEAIRLMEMSKDSLLGEKGQTARTQRPADVIFATIRELVSRGRSVHFSEAEQRCISRGFTPAQFQAALDE
  YEELNVWQVNTSRTRITFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008568
• ORF Size: 2160 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008568.1, NM_008568.2, NP_032594.1
• RefSeq Size: 2396
• RefSeq ORF: 2160
• Locus ID: 17220
• MW: 81.2 kDa
• Gene Summary: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProtKB/Swiss-Prot Function]