Tars (NM_033074) Mouse Tagged ORF Clone
CAT#: MR210282
- TrueORF®
Tars (Myc-DDK-tagged) - Mouse threonyl-tRNA synthetase (Tars)
"NM_033074" in other vectors (4)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tars |
Synonyms | D15Wsu59e; ThrRS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210282 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCAGGAGAAGGCTAGCAGCCCTTCGGGGAAGATGGACGGCGAGAAGCCGGTGGATGCCAGCGAGG AGAAGCGGAAAGAAGGAGGCAAGAAGAAGAGCAAAGATGGAGGTGGAGATGGAGGTCGAGCAGAGTTGAA TCCCTGGCCTGAATACATTAACACACGCCTTGATATGTACAACAAACTCAAAGCAGAACACGATTCTATC CTGGCCGAAAAGGCAGCAAAAGATAGCAAGCCGATTAAAGTCACCCTGCCTGACGGCAAGCAGGTGGATG CGGAGTCCTGGAAAACCACGCCGTATCAGATTGCTTGTGGAATTAGTCAGGGCCTGGCTGACAACACCGT TGTTGCTAAGGTGAACAAAGTCGTTTGGGACCTGGACCGCCCGCTGGAGACAGATTGCACGTTGGAGCTG CTCAAGTTTGAGGACGAGGAAGCGCAGGCCGTATACTGGCACTCCAGTGCCCACATAATGGGCGAGGCCA TGGAGAGAGTCTATGGCGGATGTCTATGTTACGGCCCACCAATAGAAAACGGCTTCTATTATGATATGTA CCTTGAAGAAGGGGGCGTGTCCAGCAATGACTTCTCTTCCCTGGAAACTTTGTGCAAGAAAATCATTAAA GAGAAACAAACTTTCGAAAGATTGGAAGTTAAAAAGGAAACATTACTAGAAATGTTTAAGTACAACAAGT TCAAGTGCCGGATATTGAATGAGAAAGTGAATACTCCGACCACGACTGTCTACAGATGTGGCCCTCTGAT AGATCTCTGCCGGGGTCCTCATGTCCGACACACCGGCAAGATTAAGACTTTGAAGATACACAAGAATTCT TCCACGTACTGGGAAGGCAAAGCAGACATGGAGACGCTCCAGCGGATCTATGGCATTTCATTCCCTGACC CCAAGCTACTGAAAGAGTGGGAGAAGTTCCAAGAGGAAGCCAAAAACCGAGACTACAGGAAAACCGGGAG GGACCAAGAACTATATTTCTTCCATGAACTTAGCCCTGGAAGTTGCTTTTTCCTGCCCAAAGGAGCCTAC ATTTATAATACACTAATGGAATTTATCAGGAGTGAATATAGGAAAAGAGGGTTCCAGGAGGTCGTCACTC CCAACATCTTCAATAGCCGGCTCTGGATGACCTCTGGCCACTGGCAGCACTACAGCGAGAACATGTTCTC CTTTGAGGTGGAGAAGGAGCAGTTTGCACTTAAGCCCATGAACTGCCCGGGACACTGCCTGATGTTCGAT CACCGGCCACGGTCCTGGCGAGAGCTGCCCCTGCGGCTAGCTGACTTTGGTGTGCTGCATAGGAATGAGC TCTCGGGGGCTCTCACCGGGCTCACACGGGTCCGAAGATTCCAGCAGGATGACGCACACATCTTCTGTGC CATGGAGCAGATTGAAGATGAAATCAAAGGTTGTTTGGATTTTCTTCGCACAGTATATAGTGTCTTTGGA TTTTCATTTAAATTGAATCTTTCTACTCGCCCAGAAAAATTCCTTGGAGATATTGAAATATGGAACCAAG CTGAGAAACAACTTGAAAACAGTTTGAATGAGTTCGGTGAGAAGTGGGAACTAAATCCTGGAGATGGAGC CTTCTATGGGCCAAAGATTGACATACAGATAAAAGATGCCATCGGTCGCTACCACCAGTGTGCAACCATC CAGCTGGACTTTCAGTTGCCCATCAGGTTTAACCTTACTTACGTCAGCCACGATGGCGATGATAAGAAAA GGCCAGTGATTGTTCACAGAGCCATCCTGGGGTCGGTGGAAAGAATGATTGCCATCCTCACAGAAAACTA TGGGGGCAAATGGCCTTTCTGGCTCTCTCCTCGCCAGGTGATGGTGGTCCCAGTGGGACCGACATGTGAT GAATATGCCCAAAAGGTACGGCAACAATTCCATGATGCTAAATTCATGGCGGACACTGACCTGGATCCAG GCTGTACCTTGAATAAGAAGATCAGAAACGCACAGTTAGCACAGTATAACTTCATCCTTGTTGTTGGTGA AAAAGAGAAAGCCAGTGGCACGGTGAACATCCGCACGAGAGACAATAAGGTCCACGGAGAGCGGACAGTG GAGGAGACGGTGCGGCGGCTGCAGCAGCTCAAGCAGACGCGCAGCAAGCAGGCAGAGGAGGAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210282 protein sequence
Red=Cloning site Green=Tags(s) MSQEKASSPSGKMDGEKPVDASEEKRKEGGKKKSKDGGGDGGRAELNPWPEYINTRLDMYNKLKAEHDSI LAEKAAKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVVAKVNKVVWDLDRPLETDCTLEL LKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLETLCKKIIK EKQTFERLEVKKETLLEMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKTLKIHKNS STYWEGKADMETLQRIYGISFPDPKLLKEWEKFQEEAKNRDYRKTGRDQELYFFHELSPGSCFFLPKGAY IYNTLMEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKEQFALKPMNCPGHCLMFD HRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFG FSFKLNLSTRPEKFLGDIEIWNQAEKQLENSLNEFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATI QLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCD EYAQKVRQQFHDAKFMADTDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKASGTVNIRTRDNKVHGERTV EETVRRLQQLKQTRSKQAEEEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_033074 |
ORF Size | 2169 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033074.1, NM_033074.2, NM_033074.3, NP_149065.2 |
RefSeq Size | 2636 bp |
RefSeq ORF | 2169 bp |
Locus ID | 110960 |
MW | 83.4 kDa |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202075 | Tars (untagged) - Mouse threonyl-tRNA synthetase (Tars), (10ug) |
USD 210.00 |
|
MG210282 | Tars (GFP-tagged) - Mouse threonyl-tRNA synthetase (Tars) |
USD 670.00 |
|
MR210282L3 | Lenti ORF clone of Tars (Myc-DDK-tagged) - Mouse threonyl-tRNA synthetase (Tars) |
USD 810.00 |
|
MR210282L4 | Lenti ORF clone of Tars (mGFP-tagged) - Mouse threonyl-tRNA synthetase (Tars) |
USD 810.00 |
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