Tars (NM_033074) Mouse Tagged ORF Clone

CAT#: MG210282

Tars (GFP-tagged) - Mouse threonyl-tRNA synthetase (Tars)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tars
• Synonyms: D15Wsu59e; ThrRS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210282 representing NM_033074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCAGGAGAAGGCTAGCAGCCCTTCGGGGAAGATGGACGGCGAGAAGCCGGTGGATGCCAGCGAGG
  AGAAGCGGAAAGAAGGAGGCAAGAAGAAGAGCAAAGATGGAGGTGGAGATGGAGGTCGAGCAGAGTTGAA
  TCCCTGGCCTGAATACATTAACACACGCCTTGATATGTACAACAAACTCAAAGCAGAACACGATTCTATC
  CTGGCCGAAAAGGCAGCAAAAGATAGCAAGCCGATTAAAGTCACCCTGCCTGACGGCAAGCAGGTGGATG
  CGGAGTCCTGGAAAACCACGCCGTATCAGATTGCTTGTGGAATTAGTCAGGGCCTGGCTGACAACACCGT
  TGTTGCTAAGGTGAACAAAGTCGTTTGGGACCTGGACCGCCCGCTGGAGACAGATTGCACGTTGGAGCTG
  CTCAAGTTTGAGGACGAGGAAGCGCAGGCCGTATACTGGCACTCCAGTGCCCACATAATGGGCGAGGCCA
  TGGAGAGAGTCTATGGCGGATGTCTATGTTACGGCCCACCAATAGAAAACGGCTTCTATTATGATATGTA
  CCTTGAAGAAGGGGGCGTGTCCAGCAATGACTTCTCTTCCCTGGAAACTTTGTGCAAGAAAATCATTAAA
  GAGAAACAAACTTTCGAAAGATTGGAAGTTAAAAAGGAAACATTACTAGAAATGTTTAAGTACAACAAGT
  TCAAGTGCCGGATATTGAATGAGAAAGTGAATACTCCGACCACGACTGTCTACAGATGTGGCCCTCTGAT
  AGATCTCTGCCGGGGTCCTCATGTCCGACACACCGGCAAGATTAAGACTTTGAAGATACACAAGAATTCT
  TCCACGTACTGGGAAGGCAAAGCAGACATGGAGACGCTCCAGCGGATCTATGGCATTTCATTCCCTGACC
  CCAAGCTACTGAAAGAGTGGGAGAAGTTCCAAGAGGAAGCCAAAAACCGAGACTACAGGAAAACCGGGAG
  GGACCAAGAACTATATTTCTTCCATGAACTTAGCCCTGGAAGTTGCTTTTTCCTGCCCAAAGGAGCCTAC
  ATTTATAATACACTAATGGAATTTATCAGGAGTGAATATAGGAAAAGAGGGTTCCAGGAGGTCGTCACTC
  CCAACATCTTCAATAGCCGGCTCTGGATGACCTCTGGCCACTGGCAGCACTACAGCGAGAACATGTTCTC
  CTTTGAGGTGGAGAAGGAGCAGTTTGCACTTAAGCCCATGAACTGCCCGGGACACTGCCTGATGTTCGAT
  CACCGGCCACGGTCCTGGCGAGAGCTGCCCCTGCGGCTAGCTGACTTTGGTGTGCTGCATAGGAATGAGC
  TCTCGGGGGCTCTCACCGGGCTCACACGGGTCCGAAGATTCCAGCAGGATGACGCACACATCTTCTGTGC
  CATGGAGCAGATTGAAGATGAAATCAAAGGTTGTTTGGATTTTCTTCGCACAGTATATAGTGTCTTTGGA
  TTTTCATTTAAATTGAATCTTTCTACTCGCCCAGAAAAATTCCTTGGAGATATTGAAATATGGAACCAAG
  CTGAGAAACAACTTGAAAACAGTTTGAATGAGTTCGGTGAGAAGTGGGAACTAAATCCTGGAGATGGAGC
  CTTCTATGGGCCAAAGATTGACATACAGATAAAAGATGCCATCGGTCGCTACCACCAGTGTGCAACCATC
  CAGCTGGACTTTCAGTTGCCCATCAGGTTTAACCTTACTTACGTCAGCCACGATGGCGATGATAAGAAAA
  GGCCAGTGATTGTTCACAGAGCCATCCTGGGGTCGGTGGAAAGAATGATTGCCATCCTCACAGAAAACTA
  TGGGGGCAAATGGCCTTTCTGGCTCTCTCCTCGCCAGGTGATGGTGGTCCCAGTGGGACCGACATGTGAT
  GAATATGCCCAAAAGGTACGGCAACAATTCCATGATGCTAAATTCATGGCGGACACTGACCTGGATCCAG
  GCTGTACCTTGAATAAGAAGATCAGAAACGCACAGTTAGCACAGTATAACTTCATCCTTGTTGTTGGTGA
  AAAAGAGAAAGCCAGTGGCACGGTGAACATCCGCACGAGAGACAATAAGGTCCACGGAGAGCGGACAGTG
  GAGGAGACGGTGCGGCGGCTGCAGCAGCTCAAGCAGACGCGCAGCAAGCAGGCAGAGGAGGAGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210282 representing NM_033074
  Red=Cloning site Green=Tags(s)
  
  MSQEKASSPSGKMDGEKPVDASEEKRKEGGKKKSKDGGGDGGRAELNPWPEYINTRLDMYNKLKAEHDSI
  LAEKAAKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVVAKVNKVVWDLDRPLETDCTLEL
  LKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLETLCKKIIK
  EKQTFERLEVKKETLLEMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKTLKIHKNS
  STYWEGKADMETLQRIYGISFPDPKLLKEWEKFQEEAKNRDYRKTGRDQELYFFHELSPGSCFFLPKGAY
  IYNTLMEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKEQFALKPMNCPGHCLMFD
  HRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFG
  FSFKLNLSTRPEKFLGDIEIWNQAEKQLENSLNEFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATI
  QLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCD
  EYAQKVRQQFHDAKFMADTDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKASGTVNIRTRDNKVHGERTV
  EETVRRLQQLKQTRSKQAEEEF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033074
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033074.2, NP_149065.2
• RefSeq Size: 2637
• RefSeq ORF: 2169
• Locus ID: 110960