Sh2b1 (BC051978) Mouse Tagged ORF Clone

CAT#: MR210304

Sh2b1 (Myc-DDK-tagged) - Mouse SH2B adaptor protein 1 (cDNA clone MGC:62271 IMAGE:6401205)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sh2b1
• Synonyms: SH2-Bb, SH2-B, mKIAA1299
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210304 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGTGCCCCTTCCCCAGAGGATGGGGTCTTCCCTTCTCCGCCAGCGCTGCCACCACCCCCTCCCC
  CAAGTTGGCAAGAGTTCTGTGAGTCCCATGCGAGGGCAGCTGCCCTGGATCTTGCCCGACGTTTTCGCCT
  CTACCTGGCCTCCCACCCACAGTATGCAGAGCCGGGAGCAGAGGCCGCTTTTTCTGGCCGTTTTGCTGAG
  CTCTTCCTGCAGCACTTCGAAGCCGAGGTGGCTCGGGCCTCGGGCTCACTCTCCCCACCTGTCCTGGCTC
  CATTGAGCCCTGGTGTGGAAATCCCACCATCACACGACCTGTCCCTTGAGAGCTGCAGGGTGGGTGGGCC
  CCTAGCAGTGTTGGGCCCTTCTCGATCTTCTGAGGACCTGGCTGGCCCCCTTCCTTCCTCAGTCCCTTCC
  TCTACAACATCCTCAAAGCCAAAGCTCAAGAAGCGCTTCTCCCTCCGCTCAGTGGGCCGTTCAGTCAGAG
  GCTCTGTTCGAGGCATCCTGCAGTGGCGGGGTGCCGTTGACTCGCCCTCCCAAGCTGGGCCTCTGGAGAC
  CACATCCGGCCCTCCAGTTCTAGGTGGAAACAGCAACTCCAACTCCTCGGGTGGTGCTGGGACAGTTGGT
  AGGGCATTGGCTAATGATGGCACATCCCCTGGGGAGAGATGGACTCATCGATTTGAGAGGCTGAGGCTAA
  GTCGTGGAGGGGGAACCCTGAAAGACGGAGCAGGAATGATACAGAGAGAAGAGCTGCTGAGTTTCATGGG
  GGCTGAAGAGGCTGCCCCTGACCCAGCAGGAGTGGGTCGTGGAGGAGGGGCAGCTGGGCTGACCTCAGGA
  GGAGGAGGGCAGCCTCAGTGGCAAAAGTGTCGCTTACTGCTCCGGAGTGAAGGAGAAGGAGGAGGAGGAA
  GTCGCTTGGAGTTCTTTGTACCACCCAAGGCGTCCCGACCCCGTCTCAGCATTCCCTGCTCTACTATTAC
  TGATGTCCGCACAGCCACAGCCCTAGAGATGCCTGACAGGGAGAACACGTTTGTGGTTAAGGTAGAAGGC
  CCTTCAGAGTACATCCTGGAGACAAGTGATGCGCTTCATGTGAAGGCCTGGGTGTCTGACATCCAGGAAT
  GCCTAAGCCCCGGACCCTGTCCTGCTATCAGCCCCCGTCCCATGACCCTTCCCCTGGCCCCTGGGACCTC
  CTTCTTCACAAAGGATAACACAGACAGCCTGGAGTTGCCCTGCCTGAATCATTCAGAGAGTCTGCCTAGC
  CAGGATCTGCTGCTGGGACCCAGCGAGAGTAACGACCGCCTGTCGCAGGGAGCTTATGGGGGCCTCTCAG
  ACCGGCCATCGGCGTCCTTCTCCCCTAGTTCTGCCTCCATTGCTGCCTCCCATTTTGATTCGATGGAACT
  GCTTCCTCCAGAATTGCCTCCTCGAATTCCCATTGAGGAGGGGCCTCCAGCAGGGACAGTTCATCCCCTC
  TCTACCCCCTACCCTCCCCTGGATACTCCAGAAGCAGCCACAGGATCGTTCCTGTTCCAAGGGGAGTCAG
  AGGGGGGTGAGGGGGATCAGCCCCTCTCAGGCTATCCTTGGTTCCACGGCATGCTCTCTCGACTCAAAGC
  TGCCCAGTTAGTGTTAGAAGGAGGCACTGGCTCCCATGGTGTCTTCTTGGTACGCCAGAGTGAGACAAGG
  CGTGGTGAATATGTCCTCACTTTCAACTTCCAGGGCAAGGCCAAGCACCTGCGCTTGTCACTAAATGAGG
  AGGGACAGTGCCGGGTCCAACATCTGTGGTTCCAGTCCATTTTCGATATGCTTGAGCACTTCCGGGTGCA
  CCCCATCCCTCTGGAGTCTGGAGGCTCCAGTGATGTTGTCCTTGTCAGCTATGTGCCCTCCCAGCGGCAG
  CAGGGTGAGCAGAGCAGGTCTGCAGGGGAGGAGGTGCCCGTGCACCCAAGAAGTGAGAACGGAGCACCTC
  CCGTGACCCAGCCCAGCCCTCTGAACCCCCTCCATGGACAGATCCCCCACATCCTGGGGCAGAAGAGGCG
  TCGGGGGCGCCAGAAGTTGCGGCAGCCACAGCCGCAGCAGCCAAAGAGAGGCAAGAGAAAGAGAAAGCGG
  GCAGTGGAGGGGTCCAGGAAGAGCTGGTCCCCGTGGCTGAGCTGGTCCCCATGGTTGAATTGGAAGAGGC
  CA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210304 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNGAPSPEDGVFPSPPALPPPPPPSWQEFCESHARAAALDLARRFRLYLASHPQYAEPGAEAAFSGRFAE
  LFLQHFEAEVARASGSLSPPVLAPLSPGVEIPPSHDLSLESCRVGGPLAVLGPSRSSEDLAGPLPSSVPS
  STTSSKPKLKKRFSLRSVGRSVRGSVRGILQWRGAVDSPSQAGPLETTSGPPVLGGNSNSNSSGGAGTVG
  RALANDGTSPGERWTHRFERLRLSRGGGTLKDGAGMIQREELLSFMGAEEAAPDPAGVGRGGGAAGLTSG
  GGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSTITDVRTATALEMPDRENTFVVKVEG
  PSEYILETSDALHVKAWVSDIQECLSPGPCPAISPRPMTLPLAPGTSFFTKDNTDSLELPCLNHSESLPS
  QDLLLGPSESNDRLSQGAYGGLSDRPSASFSPSSASIAASHFDSMELLPPELPPRIPIEEGPPAGTVHPL
  STPYPPLDTPEAATGSFLFQGESEGGEGDQPLSGYPWFHGMLSRLKAAQLVLEGGTGSHGVFLVRQSETR
  RGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSQRQ
  QGEQSRSAGEEVPVHPRSENGAPPVTQPSPLNPLHGQIPHILGQKRRRGRQKLRQPQPQQPKRGKRKRKR
  AVEGSRKSWSPWLSWSPWLNWKRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC051978
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC051978, AAH51978
• RefSeq Size: 2902 bp
• RefSeq ORF: 2174 bp
• Locus ID: 20399
• MW: 77.6 kDa