Mthfd1l (BC030437) Mouse Tagged ORF Clone

CAT#: MR210353

Mthfd1l (Myc-DDK-tagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mthfd1l
• Synonyms: 2410004L15Rik|AI647056|Fthfsdc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210353 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATGAGCTGCCCCTGGGCAACACCTCAGCTTCCAGACAAGCTTCGAGAGGCAGACATTGTGGTCT
  TAGGCTCACCTAAGCCAGAAGAGATTCCTGCTGCGTGGATTCCATCAGGAACCACCATTCTCAATTGCTT
  CCATGACTTCTTGTCAGGGAAGCTGAGCGGAGGTTCTCCTGGGGTCCCCGTGGACAAACTCATTGCAGAA
  GAGAGCGTGAGTCTCCTTGCTGCGGCTCTGCGCATTCAGAACATGGTGAGCAGCGGCAGGAGGTGGCTCA
  GAGAGCAGCAACATCGAAGATGGCGACTGCACTGCTTGAAACTGCAGCCTCTCTCACCTGTGCCCAGTGA
  TATTGAGATTTCTCGAGGACAGACTCCAAAAGCTGTGGATGTCCTTGCCAAGGAGATAGGATTGCTTGCA
  GATGAAATTGAAATCTATGGCAAGAGCAAAGCCAAAGTCCAATTGTCCCTGCTGGAGAGGTTAAAGGATC
  AAACAGATGGAAAATACGTCTTGGTTGCTGGGATCACGCCCACCCCGCTCGGAGAAGGGAAGAGTACAGT
  CACCATCGGACTAGTGCAGGCGCTGACCGCTCACCTGAAAGTCAACTCCTTCGCCTGTCTGAGGCAGCCT
  TCTCAGGGACCCACTTTTGGAGTGAAAGGAGGAGCTGCAGGAGGTGGATATGCTCAGGTCATCCCCATGG
  AGGAGTTCAACCTTCACCTGACTGGGGACATCCATGCCATCACTGCCGCTAATAACTTGCTGGCCGCAGC
  CATCGACACGAGAATTTTACATGAGAGCACTCAAACAGACAAGGCTCTCTACAATCGACTGGTTCCCTTA
  GTGAATGGTGTCAGAGAATTTTCAGAAATTCAACTTTCTCGGCTGAAGAAACTGGGGATACATAAGACTG
  ATCCAAGCACACTGACCGAGGAGGAAGTGAGGAAGTTCGCCCGCCTCAACATTGACCCCGCAACCATCAC
  GTGGCAGAGAGTATTGGACACGAACGACCGATTTCTGAGAAAAATAACCATTGGACAGGGAAGTACAGAG
  AAAGGATATTCCCGGCAGGCACAGTTTGACATTGCAGTGGCCAGCGAGATCATGGCCGTGCTGGCCTTGA
  CTGATAGCCTCACAGACATGAAAGAGCGGCTAGGAAGAATGGTGGTGGCCAGTGACAAAGACGGGCAGCC
  TGTGACAGCTGAGGACTTGGGGGTGACCGGCGCTCTGACAGTTTTGATGAAAGACGCCATCAAGCCAAAT
  CTGATGCAGACCCTTGAAGGAACACCTGTGTTTGTGCATGCTGGTCCCTTTGCCAACATCGCTCACGGCA
  ACTCATCAGTATTAGCTGATAAAATCGCGCTGAAATTGGTCGGCGAAGAAGGGTTTGTGGTGACTGAAGC
  TGGCTTTGGGGCTGATATTGGGATGGAGAAATTCTTCAATATCAAATGCCGAGCTTCTGGCCTGGTGCCC
  AACGTTGTCGTGCTGGTAGCCACCGTTCGAGCTCTGAAGATGCATGGAGGCGGGCCGAGTGTAACTGCTG
  GGGTTCCTCTCAAGAAAGAATATACAGAGGAGAACATTCAGCTGGTAGCTGATGGCTGCTGCAACCTACA
  GAAGCAAATTCAGATCGCTCAGCTCTTCGGGGTGCCGGTGGTGGTGGCACTGAATGTCTTCAAGACGGAC
  ACCCGTGCAGAGATTGACTTGGTGTGTGAGCTCGCTAAACGGGCCGGTGCCTTTGATGCGGTCCCCTGCT
  ATCACTGGTCGGCTGGAGGGAAAGGATCTGTGGACTTGGCCCGGGCTGTGAGAGAAGCTGCGAATAAAAG
  AAGCCGCTTCCAGTTCCTGTATGATGTTCAGCTTCCCATCGTGGAAAAGATAAGAGTCATTGCCCAGACT
  GTCTATGGAGCCAAGGACATTGAGCTGTCTCCTGAGGCGCAGTCCAAAATCGATCGCTATACGCAACAGG
  GTTTTGGAAATTTGCCCATTTGCATGGCGAAGACCCACCTCTCTCTCTCTCATGAACCTGACAAAAAGGG
  AGTGCCGAGGGATTTCACTCTGCCCATCAGTGATGTCCGGGCCAGCATCGGGGCTGGGTTCATTTATCCT
  TTGGTGGGAACGATGAGCACAATGCCAGGACTGCCCACCCGACCCTGCTTTTATGATATAGACCTTGACA
  CAGAAACGGAGCAAGTTAAAGGCTTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210353 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTMSCPWATPQLPDKLREADIVVLGSPKPEEIPAAWIPSGTTILNCFHDFLSGKLSGGSPGVPVDKLIAE
  ESVSLLAAALRIQNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLA
  DEIEIYGKSKAKVQLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLKVNSFACLRQP
  SQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRILHESTQTDKALYNRLVPL
  VNGVREFSEIQLSRLKKLGIHKTDPSTLTEEEVRKFARLNIDPATITWQRVLDTNDRFLRKITIGQGSTE
  KGYSRQAQFDIAVASEIMAVLALTDSLTDMKERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPN
  LMQTLEGTPVFVHAGPFANIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVP
  NVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQIAQLFGVPVVVALNVFKTD
  TRAEIDLVCELAKRAGAFDAVPCYHWSAGGKGSVDLARAVREAANKRSRFQFLYDVQLPIVEKIRVIAQT
  VYGAKDIELSPEAQSKIDRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFTLPISDVRASIGAGFIYP
  LVGTMSTMPGLPTRPCFYDIDLDTETEQVKGLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC030437
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC030437, AAH30437
• RefSeq Size: 3061
• RefSeq ORF: 2201
• Locus ID: 270685
• MW: 79.5 kDa