Suz12 (NM_199196) Mouse Tagged ORF Clone

CAT#: MR210406

Suz12 (Myc-DDK-tagged) - Mouse suppressor of zeste 12 homolog (Drosophila) (Suz12), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Suz12
• Synonyms: 2610028O16Rik; AI195385; AU016842; AW536442; D11Ertd530e; mKIAA0160
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210406 representing NM_199196
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCTCAGAAGCACGGCGGTGGGGGAGGGGGCGGCTCGGGGCCCAGCGCGGGGTCCGGGGGAGGCG
  GCTTCGGGGGTTCGGCGGCGGCGGTGGCGGCGGCGGCTTCGGGCGGCAAATCCGGCGGCGGGGGCTGTGG
  AGGCGGCGGCAGTTACTCGGCCTCCTCCTCCTCGGCGGCGGCCGCGGCGGCGGCGGCGGGGGCCGCGGTG
  TTGCCGGTGAAGAAGCCGAAAATGGAGCACGTCCAGGCTGACCACGAGCTTTTCCTCCAGGCCTTTGAGA
  AACCAACACAGATATATAGATTTCTTCGAACTCGAAATCTTATCGCACCAATATTTTTGCACAGAACTCT
  TACTTACATGTCTCATCGAAATTCCAGAACAAGCATCAAAAGGAAAACATTTAAAGTTGATGATATGTTA
  TCAAAAGTAGAGAAAATGAAAGGAGAGCAAGAATCTCATAGCTTGTCTGCACATTTGCAACTTACATTTA
  CCGGTTTCTTCCACAAAAATGATAAGCCATCACAAAACTCAGAAAATGAACAAAATTCTGTTACCCTGGA
  AGTCCTGCTTGTGAAAGTTTGCCACAAAAAAAGGAAGGATGTAAGTTGTCCAATAAGACAAGTCCCTACT
  GGTAAAAAGCAGGTGCCTTTGAATCCTGACCTCAATCAAACAAAACCAGGAAATTTTCCATCCCTGGCAG
  TTTCCAGTAATGAATTTGAACCTAGTAACAGCCATATGGTGAAGTCCTACTCGTTGCTGTTTAGAGTAAC
  TCGTCCAGGAAGAAGAGAATTTAATGGAATGATTAATGGAGAAACCAATGAAAATATTGATGTCAGTGAA
  GAACTTCCAGCCAGAAGAAAACGAAATCGCGAAGATGGAGAAAAGACATTTGTTGCACAAATGACAGTTT
  TTGACAAAAACAGGCGTTTACAGCTTTTAGATGGGGAATATGAAGTGGCCATGCAGGAAATGGAAGAATG
  TCCAATAAGTAAGAAAAGAGCAACATGGGAGACAATTCTTGATGGGAAGAGGCTGCCTCCATTTGAGACA
  TTTTCTCAGGGACCTACATTACAATTTACTCTTCGATGGACAGGAGAAACCAACGATAAGTCTACAGCTC
  CTGTTGCCAAGCCTCTTGCCACTAGAAATTCAGAGAGCCTTCATCAGGAAAATAAGCCTGGTTCTGTTAA
  ACCTGCACAAACTATTGCTGTTAAGGAGACGCTGACTACAGAGCTGCAAACAAGAAAAGAAAAGGATAAT
  TCAAATGAAAGTCGCCAGAAGTTAAGAATATTTTATCAGTTCCTTTATAACAATAATACAAGACAACAGA
  CAGAAGCCAGAGACGACCTGCACTGCCCGTGGTGCACTCTGAACTGCCGTAAACTGTATAGCTTACTCAA
  ACATCTAAAGCTCTGCCACAGCAGGTTCATCTTCAATTACGTATATCATCCAAAAGGTGCTAGGATAGAT
  GTTTCAATCAATGAGTGTTATGATGGCTCCTATGCAGGAAATCCTCAGGATATACATCGCCAACCTGGAT
  TTGCTTTTAGTCGAAATGGACCGGTAAAGAGAACACCTATCACACATATTCTTGTTTGCAGGCCAAAAAG
  AACAAAAGCAAGCATGTCGGAGTTTCTTGAATCTGAAGATGGAGAAGTGGAGCAGCAGAGAACATACAGC
  AGTGGCCACAATCGTCTCTATTTCCACAGTGATACCTGCTTACCTCTTCGGCCACAAGAAATGGAAGTAG
  ATAGTGAAGATGAGAAAGATCCAGAATGGCTGAGAGAAAAAACCATTACTCAAATTGAAGAATTTTCTGA
  TGTGAATGAAGGAGAGAAAGAAGTGATGAAGCTGTGGAACCTCCATGTCATGAAGCATGGATTTATTGCT
  GACAATCAAATGAATCATGCCTGTATGCTGTTTGTAGAAAATTATGGACAGAAAATAATTAAGAAGAATT
  TATGTCGAAACTTCATGCTTCATCTAGTCAGCATGCATGACTTTAATCTTATTAGCATAATGTCAATAGA
  TAAAGCTGTTACCAAGCTCCGAGAAATGCAGCAAAAACTAGAAAAAGGAGAATCTGCGACCCCTTCAAAT
  GAAGAAATAGCTGAGGAACAAAATGGAACAGCAAATGGATTCAGTGAAACTAACTCAAAAGAGAAAGCTT
  TGGAAACTGACGGTGTCTCAGGGGTTCCAAAACAGAGCAAGAAACAAAAACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210406 representing NM_199196
  Red=Cloning site Green=Tags(s)
  
  MAPQKHGGGGGGGSGPSAGSGGGGFGGSAAAVAAAASGGKSGGGGCGGGGSYSASSSSAAAAAAAAGAAV
  LPVKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTSIKRKTFKVDDML
  SKVEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSQNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPT
  GKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVSE
  ELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFET
  FSQGPTLQFTLRWTGETNDKSTAPVAKPLATRNSESLHQENKPGSVKPAQTIAVKETLTTELQTRKEKDN
  SNESRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARID
  VSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYS
  SGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIA
  DNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESATPSN
  EEIAEEQNGTANGFSETNSKEKALETDGVSGVPKQSKKQKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199196
• ORF Size: 2223 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_199196.1, NM_199196.2, NP_954666.1
• RefSeq Size: 4449
• RefSeq ORF: 2226
• Locus ID: 52615
• MW: 83.5 kDa
• Gene Summary: This gene encodes a core component of the polycomb repressive complex 2 (PRC2) that also includes, at least, embryonic ectoderm development protein (EED) and enhancer of zeste homolog 1 or 2 (EZH1 or EZH2). Through the methyltransferase activity of EZH1 or EZH2, the PRC2 complex methylates Lys9 and Lys27 of histone 3 and Lys26 of histone 1, leading to recruitment of the PRC1 complex, histone 2A ubiquitylation and transcriptional repression of the target genes. This gene product is essential for the activity and integrity of the PRC2 complex, and is required for X chromosome inactivation, stem cell maintenance and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]