Med25 (NM_029365) Mouse Tagged ORF Clone

CAT#: MR210426

Med25 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Med25
• Synonyms: 2610034E13Rik; 2610529E18Rik; ESTM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210426 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCCCGGATCCGAAGGCCCGGCGCGGGCCGGGGGCCTGGTCGCCGATGTGGTGTTTGTGATCGAGG
  GGACAGCAAACCTGGGGCCGTACTTCGAGGAGCTCCGCAAGCACTACCTGCTGCCTGCCATAGAGTACTT
  CAACGGGGGACCCCCTGCAGAGACGGACTTTGGGGGAGACTATGGTGGAACCCAGTACAGCCTTGTGGTG
  TTCAACACCGTGGACTGCGCTCCAGAGTCCTATGTACAATGTCACGCACCTACCAGCAGTGCCTATGAGT
  TTGTCACCTGGCTCGATGGCATCAAGTTCATGGGCGGTGGTGGCGAGAGCTGTAGTCTCATCGCTGAAGG
  TCTGAGCACTGCCCTGCAGCTGTTCGATGACTTTAAGAAGATGCGTGAACAGATTGGCCAGACCCACCGA
  GTCTGTCTCCTCATCTGCAACTCACCCCCGTACCTCCTGCCCGCCGTGGAGAGCACCACCTACTCAGGCT
  GCACTACCGAGAGCCTGGTGCAGAAAATAGGAGAGCGAGGCATCCACTTCTCCATCGTTTCTCCGAGAAA
  GCTGCCAGCCCTCCGGCTTCTGTTTGAGAAGGCGGCTCCTCCTGCCCTACTGGAGCCTTTGCAGCAACCA
  GCAGACGTCAGCCAGGACCCCAGGCACATGGTGCTTGTGCGTGGGCTCGTGCTGCCTGTTGGTGGCAGCT
  CGACCTCAGGCTCTCTGCAGACCAAGCAAGCAGTCCCCTTGCCACCAGCTCCAGCTTCTGCTGCCACGCT
  GTCAGCAGCGCCCCCGCAGGCTCTGCCCCCTGTCCCACCACAGTACCAGGTTCCTGGGAACCTAAGTGCT
  GCTCAGGTGGCTGCGCAGAATGCTGTGGAGGCTGCCAAGAGCCAGAAGGCCGGGCTGGGCCCACGCTTCT
  CACCCATCAACCCTCTCCAGCAAGCTGCTCCAGGAGTGGGGCCCCCCTTCAGCCAGGCCCCAGCACCCCC
  ATTGGCTCCTGTGCCCCCTGGAGCTCCAAAGCCACCTCCTGCCTCACAGCCTAGCCTAGTCTCCACCGTG
  GCCCCCGGACCAGTCCTGGCAGCTCCTGCGCAGCCTGGGGCGCCGTCTCTGGCAGGCACTGTAACCCCAG
  GTGGGGTCAATGGTCCTTCAGCAGCCCAGCTTGGGGGTCCTGCACTTGGTGGGCAGCAGTCAGTATCCAA
  CAAGCTCCTAGCCTGGAGTGGAGTCCTCGAGTGGCAGGAGAAACCCAAGCCTGCATCTGTGGATGCCAAC
  ACCAAGCTGACGAGGTCTCTGCCTTGCCAGGTCTATGTGAACCATGGAGAAAACCTGAAGACTGAGCAGT
  GGCCCCAGAAACTGATCATGCAGCTTATCCCTCAGCAGCTGCTGACCACCCTGGGTCCGCTGTTCCGGAA
  CTCAAGAATGGTTCAGTTCCACTTCACCAACAAGGACCTGGAGTCCCTCAAAGGCCTCTACCGCATCATG
  GGCAATGGCTTTGCGGGCTGCGTGCATTTCCCGCACACGGCCCCATGCGAGGTGCGCGTGCTTATGCTCC
  TGTACTCGTCTAAGAAGAAGATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTCGTCAATGGCAT
  CCGCCAGGTCATCACCAACCACAAGCAGGTCCAGCAGCAGAAGCTGGAGCAACAGCGCGGGATGGGGGCA
  CAGCAGGCACCTCCAGTCCTGGGCCCCATTCTGGAGGAGCAAGCGAGGCCCCCGCAGAATCTACTACAGC
  TCCGTGCACCACAGCCTCAGCCTCAGGGTGCCGTGGGAGCTTCTGCGGCTACAGGGCAGCCCCAGCCCCA
  AGGTGCTACCCAGGCCCCCACAGGCGCACCTCAAGGTCCTCCTGGAGCAGCCCCTGGCCCACCTCCTTCT
  GGACCCATCCTGCGGCCCCAGAACCCTGGGGCCAACCCCCAGCTGCGGAGCCTTCTCCTTAACCCAGCAC
  CGCCCCAGACTGGGGTGCCCCCGCCCCAGGCCTCCCTACACCACCTACAGCCTCCAGGGGCCCCTACATT
  GCTGCCACCACATCAGAGCATGGGGCAGCCCCAGCTGGGGCCGCAGCTCCTGCATCCTCCACCTGCCCAG
  TCATGGCCCACACAGCTTCCCCAGCGGGCGCCACTGCCAGGTCAGATGCTGCTGAGCGGGGGTCCCAGGG
  GCCCGGTCCCCCAGCCGGGCCTGCAGCCCAGCGTCATGGAGGACGACATCCTCATGGACCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210426 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVV
  FNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHR
  VCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQP
  ADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSA
  AQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTV
  APGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDAN
  TKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIM
  GNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGA
  QQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPS
  GPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQ
  SWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029365
• ORF Size: 2238 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_029365.1, NM_029365.2, NM_029365.3, NP_083641.1
• RefSeq Size: 2291
• RefSeq ORF: 2238
• Locus ID: 75613
• MW: 78.1 kDa