Setdb1 (BC079537) Mouse Tagged ORF Clone

CAT#: MR210482

Setdb1 (Myc-DDK-tagged) - Mouse SET domain, bifurcated 1 (cDNA clone MGC:90670 IMAGE:6410215)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Setdb1
• Synonyms: ESET, mKIAA0067
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210482 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCCCTCCCTGGGTGCATGAGTTTGGCTGCAGCGCCAGCTGCAGCTGACTCTGCAGAGATTGCTG
  AGCTGCAGCAGGCGGTGGTTGAAGAGCTGGGTATCTCTATGGAGGAACTTCGTCAGTACATTGATGAGGA
  ACTGGAAAAGATGGACTGCATACAGCAGCGCAAGAAGCAGCTCGCAGAGCTGGAGACGTGGGTACTACAG
  AAAGAGTCTGAAGTGGCTTATGTTGATCGGCTGTTTGATGATGCATCCAGGGAAGTGACTAACTGTGAGT
  CTTTGGTGAAGGATTTCTACTCTAAGCTGGGACTACAGTATCATGACAGTAGCTCTGAGGATGAAGCTTC
  CCGGCCCACAGAGATCATTGAGATTCCTGATGAAGATGATGATGTCCTCAGTATTGATTCAGGTGATGCT
  GGGAGCAGAACTCCAAAAGACCAGAAGCTTCGTGAAGCTATGGCTGCCTTAAGAAAATCAGCTCAAGATG
  TCCAGAAGTTCATGGATGCTGTCAACAAGAAAAGCAGTTCTCAAGATCTACATAAAGGAACCTTGGGTCA
  GGTGTCTGGAGAACTGAGCAAAGATGGGGACCTGATAGTCAGCATGCGGATTCTGGGCAAGAAGAGGACT
  AAGACATGGCACAAAGGCACCCTTATTGCCATCCAGACTGTTGGGCTAGGAAAAAAATACAAAGTGAAAT
  TTGACAACAAAGGAAAGAGTCTGCTATCTGGGAACCATATTGCCTATGATTACCACCCTCCCGCTGACAA
  GCTGTTTGTGGGCAGTCGAGTGGTGGCCAAGTACAAAGATGGAAATCAGGTCTGGCTTTATGCTGGCATT
  GTAGCTGAGACCCCTAACGTCAAGAACAAGCTCAGATTTTTAATTTTTTTTGATGATGGCTATGCTTCCT
  ATGTCACTCAGTCAGAGCTTTATCCCATTTGCCGACCACTAAAAAAGACTTGGGAGGACATAGAAGATAG
  CTCCTGCCGAGACTTCATAGAGGAATATATCACTGCCTATCCAAACCGCCCAATGGTACTTCTCAAGAGT
  GGGCAGCTTATCAAGACTGAGTGGGAAGGCACATGGTGGAAGTCTCGAGTTGAAGAGGTGGATGGCAGCC
  TAGTCAGGATCCTCTTTCTGGATGACAAAAGATGTGAGTGGATATATCGAGGCTCTACACGCCTGGAACC
  TATGTTTAGTATGAAGACATCCTCAGCCTCTGCAATGGAGAAGAAGCAAGGGGGGCAACTCAGAACCCGT
  CCTAATATGGGTGCTGTGAGGAGCAAAGGTCCTGTTGTTCAGTATACACAGGATCTAACTGGTACTGGAA
  TCCAGTTTAAGCCCATGGAGCCCCTACAGCCTATAGCTCCACCGGCCCCACTTCCTATACCTCCTCTTTC
  CCCCCAAGCAGCTGACACTGACTTAGAAAGCCAACTTGCACAATCACGGAAACAAGTAGCCAAGAAGAGC
  ACATCATTCCGACCAGGATCTGTGGGCTCCGGCCATTCCTCCCCTACTTCATCCACACTCAGTGAAAATG
  TGTCTGCTGGGAAACTTGGGATAAACCAGACATATCGGTCACCTTTGGCCTCAGTAACATCTACCCCAGC
  ATCTGCAGCCCCTCCAGTCCCTCCAGTCCCACCAGGGCCTCCAACCCCTCCAGGGCCTCCAGCTCCTCCA
  GGGCCTCTAGCTCCTCCAGCCTTCCATGGCATGTTAGAGCGGGCACCAGCTGAGCCCTCCTACCGAGCCC
  CCATGGAGAAGCTTTTCTATTTACCTCATGTCTGCAGTTACACTTGTTTGTCCCGGATCAGACCCATGAG
  AAACGAACAGTATCGGGGCAAGAACCCTCTATTAGTTCCACTTCTGTATGACTTCCGGAGGATGACAGCA
  CGGCGCAGAGTTAACCGCAAAATGGGCTTTCATGTAATCTATAAGACACCCTGTGGTCTCTGCCTTCGGA
  CGATGCAGGAGATAGAGCGCTACCTTTTTGAGACTGGCTGTGACTTTCTGTTCCTGGAGATGTTCTGTTT
  GGATCCATATGTTCTTGTTGACAGAAAGTTTCAACCCTTTAAGCCTTTTTACTATATTTTGGACATCACC
  TATGGCAAGGAAGATGTTCCCCTGTCCTGTGTTAATGAGATTGACACAACTCCCCCACCCCAGGTGGCCT
  ACAGCAAGGAACGCATTCCTGGCAAGGGTGTTTTCATTAACACAGGCCCTGAATTTCTGGTTGGCTGTGA
  CTGCAAGGATGGGTGTCGGGATAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210482 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSLPGCMSLAAAPAAADSAEIAELQQAVVEELGISMEELRQYIDEELEKMDCIQQRKKQLAELETWVLQ
  KESEVAYVDRLFDDASREVTNCESLVKDFYSKLGLQYHDSSSEDEASRPTEIIEIPDEDDDVLSIDSGDA
  GSRTPKDQKLREAMAALRKSAQDVQKFMDAVNKKSSSQDLHKGTLGQVSGELSKDGDLIVSMRILGKKRT
  KTWHKGTLIAIQTVGLGKKYKVKFDNKGKSLLSGNHIAYDYHPPADKLFVGSRVVAKYKDGNQVWLYAGI
  VAETPNVKNKLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDSSCRDFIEEYITAYPNRPMVLLKS
  GQLIKTEWEGTWWKSRVEEVDGSLVRILFLDDKRCEWIYRGSTRLEPMFSMKTSSASAMEKKQGGQLRTR
  PNMGAVRSKGPVVQYTQDLTGTGIQFKPMEPLQPIAPPAPLPIPPLSPQAADTDLESQLAQSRKQVAKKS
  TSFRPGSVGSGHSSPTSSTLSENVSAGKLGINQTYRSPLASVTSTPASAAPPVPPVPPGPPTPPGPPAPP
  GPLAPPAFHGMLERAPAEPSYRAPMEKLFYLPHVCSYTCLSRIRPMRNEQYRGKNPLLVPLLYDFRRMTA
  RRRVNRKMGFHVIYKTPCGLCLRTMQEIERYLFETGCDFLFLEMFCLDPYVLVDRKFQPFKPFYYILDIT
  YGKEDVPLSCVNEIDTTPPPQVAYSKERIPGKGVFINTGPEFLVGCDCKDGCRDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC079537
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC079537, AAH79537
• RefSeq Size: 5687 bp
• RefSeq ORF: 2267 bp
• Locus ID: 84505
• MW: 84.4 kDa