Postn (BC031449) Mouse Tagged ORF Clone

CAT#: MR210633

Postn (Myc-DDK-tagged) - Mouse periostin, osteoblast specific factor (cDNA clone MGC:25368 IMAGE:4457222)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Postn
• Synonyms: OSF-2, Osf2, Periostin, PN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210633 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCCTCTCCTGCCCTTATATGCTCTGCTGCTGCTGTTCCTGTGTGATATTAACCCTGCAAATGCCA
  ACAGTTACTATGACAAGGTCCTGGCTCACAGCCGCATCAGGGGTCGGGATCAGGGCCCAAACGTCTGTGC
  CCTCCAGCAAATTCTGGGCACCAAAAAGAAATACTTCAGCTCCTGTAAGAACTGGTATCAAGGTGCTATC
  TGCGGGAAGAAAACCACTGTGCTATATGAATGCTGCCCTGGCTATATGAGAATGGAAGGGATGAAAGGCT
  GCCCCGCAGTGATGCCTATTGACCATGTTTATGGCACGCTGGGCATTGTGGGAGCCACTACCACTCAGCA
  CTACTCCGATGTCTCGAAGCTGAGAGAAGAGATTGAAGGAAAAGGGTCATGCACGTACTTCGCGCCGAGT
  AACGAGGCTTGGGAGAACCTGGATTCTGACATTCGCAGAGGACTGGAGAACAATGTCAATGTTGAGCTAC
  TGAATGCCTTACACAGCCACATGGTTAATAAGAGAATGTTAACCAAGGACCTGAAACACGGCATGGTTAT
  TCCTTCAATGTACAACAATCTGGGGCTTTTTATTAACCATTATCCCAATGGGGTTGTCACTGTGAACTGT
  GCTCGAGTCATCCATGGGAACCAGATTGCCACAAATGGTGTCGTCCATGTCATTGACCGTGTCCTGACAC
  AAATTGGTACCTCCATCCAAGACTTCCTTGAAGCAGAAGACGACCTTTCATCATTTAGAGCAGCCGCCAT
  CACCTCTGACCTCTTGGAGTCCCTTGGAAGAGATGGTCACTTCACGCTCTTTGCTCCCACCAATGAAGCT
  TTCGAGAAACTGCCACGAGGTGTCCTAGAAAGGATCATGGGAGACAAAGTGGCTTCTGAAGCTCTCATGA
  AGTACCACATCCTAAATACCCTCCAGTGCTCTGAGGCCATCACTGGAGGAGCCGTGTTTGAGACCATGGA
  AGGAAACACTATTGAGATAGGGTGCGAAGGGGACAGTATCTCCATTAACGGAATCAAGATGGTGAACAAG
  AAAGACATTGTGACTAAGAATGGTGTCATCCACCTGATTGATGAAGTCCTCATTCCTGATTCTGCCAAAC
  AAGTTATTGAGCTGGCTGGAAAACAGCAAACCACTTTCACCGACCTGGTAGCCCAATTAGGCTTGGCATC
  CTCTCTGAAGCCAGATGGAGAGTACACCTTATTAGCACCTGTGAACAATGCGTTCTCTGATGACACTCTG
  AGCATGGACCAACGCCTTCTTAAGCTAATTCTGCAAAATCACATATTGAAAGTAAAAGTTGGCCTTAGCG
  ACCTCTACAATGGACAGATACTGGAAACCATTGGAGGCAAACAACTCCGAGTCTTTGTGTATCGGACGGC
  TATCTGCATAGAAAACTCATGCATGGTGAGAGGAAGCAAGCAGGGAAGGAATGGTGCCATTCACATATTC
  CGAGAAATCATCCAACCAGCAGAGAAATCCCTGCACGACAAGCTGCGGCAAGACAAGCGCTTTAGCATCT
  TCCTCAGCCTCCTTGAAGCTGCAGATTTGAAAGATCTCCTGACACAGCCCGGAGATTGGACCTTGTTTGC
  ACCAACCAATGATGCCTTCAAGGGAATGACTAGCGAAGAAAGGGAGCTTCTGATTGGGGATAAAAATGCT
  CTCCAAAACATCATTCTTTATCACCTGACCCCAGGGGTTTATATTGGAAAGGGATTCGAACCCGGAGTCA
  CTAATATCCTGAAGACCACACAGGGAAGCAAAATCTATCTGAAAGGAGTAAACGAAACGCTTCTAGTGAA
  TGAGTTGAAGTCCAAAGAATCTGACATCATGACGACAAATGGTGTCATCCACGTCGTGGACAAACTCCTC
  TATCCAGCAGATATTCCAGTTGGAAATGATCAGCTCTTGGAATTACTGAACAAACTGATAAAATACATCC
  AAATCAAGTTTGTTCGTGGCAGCACCTTCAAAGAAATCCCCATGACTGTCTATAGACCTGCAATGACGAA
  GATCCAAATTGAAGGTGATCCCGACTTCAGGCTGATTAAAGAAGGCGAAACGGTGACAGAAGTGATCCAC
  GGAGAGCCAGTCATTAAAAAGTACACCAAAATCATAGATGGAGTTCCTGTTGAAATAACTGAAAAACAGA
  CTCGGGAAGAACGAATCATTACAGGTCCTGAGATAAAATATACCAGGATTTCCACAGGAGGTGGAGAAAC
  AGGAGAGACCTTGCAGAAATTCTTGCAAAAAGACACACCTGCAAAGAAGATACCAGCCAACAAAAGGGTT
  CAAGGGCCTAGAAGACGATCAAGAGAAGGCCGTTCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210633 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVPLLPLYALLLLFLCDINPANANSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAI
  CGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSCTYFAPS
  NEAWENLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNC
  ARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFLEAEDDLSSFRAAAITSDLLESLGRDGHFTLFAPTNEA
  FEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNK
  KDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTL
  SMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIF
  REIIQPAEKSLHDKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTSEERELLIGDKNA
  LQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYLKGVNETLLVNELKSKESDIMTTNGVIHVVDKLL
  YPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYRPAMTKIQIEGDPDFRLIKEGETVTEVIH
  GEPVIKKYTKIIDGVPVEITEKQTREERIITGPEIKYTRISTGGGETGETLQKFLQKDTPAKKIPANKRV
  QGPRRRSREGRSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031449
• ORF Size: 2349 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC031449, AAH31449
• RefSeq Size: 3109
• RefSeq ORF: 2351
• Locus ID: 50706
• MW: 87 kDa
• Gene Summary: This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]