Kifap3 (BC049100) Mouse Tagged ORF Clone

CAT#: MR210688

Kifap3 (Myc-DDK-tagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kifap3
• Synonyms: KAP3, SMAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210688 representing BC049100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGGCGAGGACGCCAGATACCTCAAAAGGAAAGTTAAAGGGGGGAATATTGATGTACATCCATCAG
  AAAAAGCTCTCATTGTTCAGTATGAAGTGGAAGCTACCATTCTTGGAGAAATGGGAGATCCCATGTTGGG
  AGAACGAAAGGAATGCCAAAAAATCATCCGTCTGAAGAGTCTCAATGCTAACACAGACATCACCTCTCTG
  GCCCGGAAGGTGGTTGAGGAATGTAAGCTCATCCATCCCTCAAAGCTAAGCGAGGTGGAGCAGCTTTTGT
  ACTATCTACAGAACCGCCGAGACTCATTGCCGGGAAAAGAAAAAAAAGAAAAATCAAGCAAGCCTAAAGA
  CCCACCTCCTTTTGAAGGGATGGAGATTGACGAAGTGGCCAACATTAATGACATGGACGAATACATTGAG
  CTCTTGTATGAAGACATTCCAGATAAGGTTCGGGGTTCTGCCTTGATCCTACAGCTTGCTCGAAATCCTG
  ATAACCTGGAAGAGCTATTATTAAATGAAACTGCCCTGGGTGCGCTAGCAAGAGTCCTGAGAGAAGACTG
  GAAACAAAGTGTTGAGTTAGCTACAAACATCATTTATATCTTCTTTTGCTTTTCTAGCTTTTCTCATTTT
  CATGGACTCATCACTCACTACAAAATTGGAGCACTGTGTATGAATATCATTGATCATGAGTTAAAAAGAC
  ACGAGCTTTGGCAAGAAGAACTCTCTAAGAAGAAGAAAGCTGTTGATGAAGACCTTGAAAATCAAACATT
  GAGAAAGGATTATGACAAAACCTTTAAAAAATACCAAGGACTTGTGGTAAAACAAGAGCAGCTACTGAGA
  GTTGCTCTCTACTTGCTTTTGAATCTTGCGGAGGACACACGTACAGAACTGAAGATGAGGAACAAGAATA
  TCGTTCACATGTTGGTGAAGGCTCTTGATCGGGACAATTTTGAGCTGCTGATTCTGGTCGTGTCATTCTT
  AAAGAAACTGAGTATTTTTATGGAGAATAAAAATGACATGGTAGAGATGGATATTGTTGAAAAACTGGTA
  AAAATGATACCCTGTGAGCATGAAGATCTCCTGAATATCACCCTCCGGCTCCTCTTAAACCTCTCGTTTG
  ACACAGGCCTGAGGAACAAGATGGTACAAGTTGGGCTTCTCCCAAAGCTCACTGCACTCCTGGGCAATGA
  AAACTACAAACAAATAGCAATGTGTGTGCTTTACCATATAAGCATGGATGACCGCTTTAAGTCAATGTTT
  GCATATACTGACTGCATACCACAGTTAATGAAGATGCTCTTTGAATGTTCAGATGAACGAATTGACTTGG
  AGCTGATTTCTTTCTGCATTAATCTTGCTGCTAACAAGAGAAATGTCCAGCTCATCTGTGAAGGAAATGG
  GCTGAAAATGCTCATGAAAAGAGCTCTGAAGCTCAAGGACCCACTGCTGATGAAGATGATCAGAAACATC
  TCCCAGCATGATGGGCCCACTAAGAATTTGTTCATTGATTATGTTGGGGACCTTGCAGCCCAGATTTCCA
  GTGATGAAGAGGAGGAGTTTGTAATCGAGTGTTTGGGAACGCTGGCAAATCTGACAATCCCAGATCTAGA
  CTGGGAACTGGTCCTGAAGGAGTACAAGCTGGTTCCATTCCTCAAAGACAAACTAAAGCCAGGTGCCGCA
  GAGGATGACCTTGTTTTAGAAGTGGTTATCATGATTGGGACGGTGTCTATGGATGACTCTTGTGCTGCCC
  TACTGGCCAAATCTGGGATAATCCCAGCCCTCATCGAGCTGCTGAACGCTCAACAAGAAGATGATGAATT
  TGTGTGTCAAATAATCTATGTCTTCTACCAGATGGTGTTCCATCAGGCCACAAGAGATGTCATAATCAAG
  GAAACACAAGCTCCAGCATATCTCATTGACCTGATGCATGATAAAAATAATGAAATCCGGAAAGTCTGTG
  ATAACACATTAGATATCATCGCAGAGTATGATGAAGAGTGGGCCAAGAAAATTCAGAGTGAGAAGTTTCG
  CTGGCATAACTCTCAGTGGCTGGAGATGGTGGAGAGCCGTCAGCTGGATGAGAGCGAGCAGTACTTGTAT
  GGTGATGATCGCATTGAGCCGTACATCCATGAAGGGGACATTCTTGAAAGGCCTGACCTTTTCTACAACT
  CAGACGGACTAATTACCTCTGAAGGAGCCATAAGTCCAGACTTCTTCAATGATTTTCACCTCCAGAATGG
  AGATGTGGTGGGGCAACACGCATTTCCTGGCAGCCTCGGGATGGATGGCTTCGGGCAGCCACTGGGGATC
  CTTGGACGTCCTGCCACCGCGTATGGATTCCGCCCTGACGAACCTTACTACTACAGCTTTGGGTCTCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210688 representing BC049100
  Red=Cloning site Green=Tags(s)
  
  MQGEDARYLKRKVKGGNIDVHPSEKALIVQYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSL
  ARKVVEECKLIHPSKLSEVEQLLYYLQNRRDSLPGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIE
  LLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHF
  HGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDLENQTLRKDYDKTFKKYQGLVVKQEQLLR
  VALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLV
  KMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNENYKQIAMCVLYHISMDDRFKSMF
  AYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKLKDPLLMKMIRNI
  SQHDGPTKNLFIDYVGDLAAQISSDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPFLKDKLKPGAA
  EDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIK
  ETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQLDESEQYLY
  GDDRIEPYIHEGDILERPDLFYNSDGLITSEGAISPDFFNDFHLQNGDVVGQHAFPGSLGMDGFGQPLGI
  LGRPATAYGFRPDEPYYYSFGSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC049100
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC049100.1
• RefSeq Size: 3952
• RefSeq ORF: 2381
• Locus ID: 16579
• MW: 144.9 kDa
• Gene Summary: The protein encoded by this gene is the non-motor subunit of kinesin-2 complex, and forms a heterotrimer with two members of the kinesin superfamily of proteins that together form a microtubule plus-end directed translocator that plays an important role in intracellular transport, mitosis, and cell-cell adhesion. This protein contains multiple armadillo repeats involved in protein binding, and may serve as an adaptor to regulate binding of cargo with the motor proteins. Conditional disruption of this gene in mouse neural precursor cells caused a tumor-like phenotype and defective organization of the neuroepithelium thought to be the result of altered N-cadherin subcellular localization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]