Ppargc1a (NM_008904) Mouse Tagged ORF Clone

CAT#: MR210710

Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppargc1a
• Synonyms: A830037N07Rik; Gm11133; PGC-1; Pgc-1alpha; Pgc1; Pgco1; PPARGC-1-alpha; Ppargc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210710 representing NM_008904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGGGACATGTGCAGCCAAGACTCTGTATGGAGTGACATAGAGTGTGCTGCTCTGGTTGGTGAGG
  ACCAGCCTCTTTGCCCAGATCTTCCTGAACTTGACCTTTCTGAACTTGATGTGAATGACTTGGATACAGA
  CAGCTTTCTGGGTGGATTGAAGTGGTGTAGCGACCAATCGGAAATCATATCCAACCAGTACAACAATGAG
  CCTGCGAACATATTTGAGAAGATAGATGAAGAGAATGAGGCAAACTTGCTAGCGGTCCTCACAGAGACAC
  TGGACAGTCTCCCCGTGGATGAAGACGGATTGCCCTCATTTGATGCACTGACAGATGGAGCCGTGACCAC
  TGACAACGAGGCCAGTCCTTCCTCCATGCCTGACGGCACCCCTCCCCCTCAGGAGGCAGAAGAGCCGTCT
  CTACTTAAGAAGCTCTTACTGGCACCAGCCAACACTCAGCTCAGCTACAATGAATGCAGCGGTCTTAGCA
  CTCAGAACCATGCAGCAAACCACACCCACAGGATCAGAACAAACCCTGCCATTGTTAAGACCGAGAATTC
  ATGGAGCAATAAAGCGAAGAGCATTTGTCAACAGCAAAAGCCACAAAGACGTCCCTGCTCAGAGCTTCTC
  AAGTATCTGACCACAAACGATGACCCTCCTCACACCAAACCCACAGAAAACAGGAACAGCAGCAGAGACA
  AATGTGCTTCCAAAAAGAAGTCCCATACACAACCGCAGTCGCAACATGCTCAAGCCAAACCAACAACTTT
  ATCTCTTCCTCTGACCCCAGAGTCACCAAATGACCCCAAGGGTTCCCCATTTGAGAACAAGACTATTGAG
  CGAACCTTAAGTGTGGAACTCTCTGGAACTGCAGGCCTAACTCCTCCCACAACTCCTCCTCATAAAGCCA
  ACCAAGATAACCCTTTCAAGGCTTCGCCAAAGCTGAAGCCCTCTTGCAAGACCGTGGTGCCACCGCCAAC
  CAAGAGGGCCCGGTACAGCGAGTGTTCTGGTACCCAAGGCAGCCACTCCACCAAGAAAGGGCCCGAGCAA
  TCTGAGTTGTACGCACAACTCAGCAAGTCCTCAGGGCTCAGCCGAGGACACGAGGAAAGGAAGACTAAAC
  GGCCCAGTCTTCGGCTGTTTGGTGACCATGACTATTGTCAGTCACTCAATTCCAAAACGGATATACTCAT
  TAACATATCACAGGAGCTCCAAGACTCTAGACAACTAGACTTCAAAGATGCCTCCTGTGACTGGCAGGGG
  CACATCTGTTCTTCCACAGATTCAGGCCAGTGCTACCTGAGAGAGACTTTGGAGGCCAGCAAGCAGGTCT
  CTCCTTGCAGCACCAGAAAACAGCTCCAAGACCAGGAAATCCGAGCGGAGCTGAACAAGCACTTCGGTCA
  TCCCTGTCAAGCTGTGTTTGACGACAAATCAGACAAGACCAGTGAACTAAGGGATGGCGACTTCAGTAAT
  GAACAATTCTCCAAACTACCTGTGTTTATAAATTCAGGACTAGCCATGGATGGCCTATTTGATGACAGTG
  AAGATGAAAGTGATAAACTGAGCTACCCTTGGGATGGCACGCAGCCCTATTCATTGTTCGATGTGTCGCC
  TTCTTGCTCTTCCTTTAACTCTCCGTGTCGAGACTCAGTGTCACCACCGAAATCCTTATTTTCTCAAAGA
  CCCCAAAGGATGCGCTCTCGTTCAAGATCCTTTTCTCGACACAGGTCGTGTTCCCGATCACCATATTCCA
  GGTCAAGATTAAGGTCCCCAGGCAGTAGATCCTCTTCAAGATCCTGTTACTACTATGAATCAAGCCACTA
  CAGACACCGCACACACCGCAATTCTCCCTTGTATGTGAGATCACGTTCAAGGTCACCCTACAGCTGTAGG
  CCCAGGTACGACAGCTATGAAGCCTATGAGCACGAAAGGCTCAAGAGGGATGAATACCGCAAAGAGCACG
  AGAAGCGGGAGTCTGAAAGGGCCAAGCAGAGAGAGAGGCAGAAGCAGAAAGCAATTGAAGAGCGCCGTGT
  GATTTACGTTGGTAAAATCAGACCTGACACAACGCGGACAGAATTGAGAGACCGCTTTGAAGTTTTTGGT
  GAAATTGAGGAATGCACCGTAAATCTGCGGGATGATGGAGACAGCTATGGTTTCATCACCTACCGTTACA
  CCTGTGACGCTTTCGCTGCTCTTGAGAATGGATATACTTTACGCAGGTCGAACGAAACTGACTTCGAGCT
  GTACTTTTGTGGACGGAAGCAATTTTTCAAGTCTAACTATGCAGACCTAGATACAAACTCAGACGATTTT
  GACCCTGCTTCCACCAAGAGCAAGTATGACTCTCTGGATTTTGATAGTTTACTGAAGGAAGCTCAGAGAA
  GCTTGCGCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210710 representing NM_008904
  Red=Cloning site Green=Tags(s)
  
  MAWDMCSQDSVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNE
  PANIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGAVTTDNEASPSSMPDGTPPPQEAEEPS
  LLKKLLLAPANTQLSYNECSGLSTQNHAANHTHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELL
  KYLTTNDDPPHTKPTENRNSSRDKCASKKKSHTQPQSQHAQAKPTTLSLPLTPESPNDPKGSPFENKTIE
  RTLSVELSGTAGLTPPTTPPHKANQDNPFKASPKLKPSCKTVVPPPTKRARYSECSGTQGSHSTKKGPEQ
  SELYAQLSKSSGLSRGHEERKTKRPSLRLFGDHDYCQSLNSKTDILINISQELQDSRQLDFKDASCDWQG
  HICSSTDSGQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPCQAVFDDKSDKTSELRDGDFSN
  EQFSKLPVFINSGLAMDGLFDDSEDESDKLSYPWDGTQPYSLFDVSPSCSSFNSPCRDSVSPPKSLFSQR
  PQRMRSRSRSFSRHRSCSRSPYSRSRLRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSCR
  PRYDSYEAYEHERLKRDEYRKEHEKRESERAKQRERQKQKAIEERRVIYVGKIRPDTTRTELRDRFEVFG
  EIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDTNSDDF
  DPASTKSKYDSLDFDSLLKEAQRSLRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008904
• ORF Size: 2391 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008904.1, NM_008904.2, NP_032930.1
• RefSeq Size: 3029
• RefSeq ORF: 2394
• Locus ID: 19017
• MW: 91 kDa
• Gene Summary: This gene encodes a transcriptional coactivator that induces and coordinates gene expression regulating mitochondrial biogenesis, respiration, hepatic gluconeogenesis, thermogenic program in brown fat and muscle fiber-type switching. Mice lacking the encoded protein exhibit reduced thermogenic capacity, hyperactivity and resistance to diet-induced obesity. Mice lacking the encoded protein specifically in the heart exhibit peripartum cardiomyopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]