Dpp6 (BC048383) Mouse Tagged ORF Clone

CAT#: MR210746

Dpp6 (Myc-DDK-tagged) - Mouse dipeptidylpeptidase 6 (cDNA clone MGC:56837 IMAGE:6306267)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dpp6
• Synonyms: D5Buc3, D5Buc5, D5Buc4, Rw
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210746 representing BC048383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACAGCCAAGGAGCCCAGCGCCTCAGGAAAATCTGTACAGCAGCAGGATCAGGAGCTGGTGGGGA
  GTAATCCTCCACAGAGGAACTGGAAAGGAATCGCCATTGCGCTGCTCGTCATCCTTGTCATCTGCTCTCT
  GATTGTCACCTCAGTCATCCTGTTGACCCCAGCGGAAGATACCAGTCTGTCTCAGAAGAAAAAAGTGACT
  GTGGAAGATCTCTTCAGTGAAGACTTCAAAATCCATGACCCAGAGGCAAAGTGGATAAGCAATAAGGAAT
  TCATCTACAGAGAACGGAAAGGGAGTGTCATACTGCGGAATGTTGAAACAAATAATTCCACGGTGTTAAT
  AGAAGGCAAAAAAATTGAATCCTTGAGAGCCATCAGATATGAAATATCCCCAGATAAAGAGTATGTGCTG
  TTCTCCTATAATGTGGAGCCGGTATACCAACACTCCCACACTGGATATTATGTTCTGAGCAAAATTCCTC
  ATGGGGACCCTCAGAGTCTGGACCCACCAGAAGTCAGCAACGCAAAGCTTCAGTATGCAGGGTGGGGCCC
  AAAAGGCCAGCAGCTGATATTTATCTTTGAGAACAACATCTACTACTGTGCACACGTTGGAAAGCAGGCA
  ATTCGAGTGGTCTCTACAGGGAAGGAGGGCGTGATCTACAATGGCCTCAGTGACTGGCTGTATGAAGAGG
  AGATTCTGAAGAGCCACATCGCTCATTGGTGGTCACCAGATGGTACAAGGCTCGCCTATGCCACCATCAA
  CGACTCCCGGGTTCCACTTATGGAGCTGCCAACGTACACAGGCTCTGTCTACCCCACTGTGAAGCCCTAC
  CACTATCCTAAGGCTGGCAGTGAAAACCCCAGCATCTCGCTCCATGTCATCGGCTTGAATGGACCCACCC
  ACGACCTGGAGATGATGCCACCTGATGACCCGCGCATGAGGGAATATTACATCACCATGGTGAAATGGGC
  CACCAGCACCAAGGTCGCTGTGACCTGGCTGAACCGGGCCCAGAATGTGTCAATCCTGACCCTCTGTGAT
  GCCACCACGGGTGTCTGCACTAAGAAACATGAAGATGAAAGCGAAGCCTGGCTCCACAGACAGAATGAAG
  AGCCTGTGTTCTCCAAAGATGGCAGGAAGTTTTTCTTTGTCAGAGCGATCCCACAGGGAGGCCGGGGTAA
  ATTCTACCATATCACCGTATCATCATCCCAGCCCAACAGCAGCAATGACAACATCCAGTCTATCACCTCT
  GGAGACTGGGATGTGACCAAGATCCTGTCCTATGATGAGAAGCGGAATAAGATCTATTTCCTGAGCACAG
  AAGACCTGCCACGGAGACGACATCTCTACAGTGCCAACACGGTAGATGACTTCAACAGGCAGTGTCTGTC
  ATGTGACTTGGTGGAGAACTGCACCTATGTCAGCGCTTCTTTCAGCCATAACATGGACTTCTTCCTGCTC
  AAGTGTGAAGGCCCTGGCGTACCCACTGTTACTGTGCATAACACCACGGACAAGAGAAGAATGTTTGACT
  TGGAAGCAAATGAGGAAGTTCAGAAGGCTATCAACGACCGGCAGATGCCGAAGATTGAATATCGAAAAAT
  TGAAGTAGAAGATTACAGCTTGCCAATGCAAATCTTGAAGCCGGCCACCTTCACCGACACAGCTCACTAC
  CCCTTATTGCTCGTGGTGGATGGTACCCCAGGGAGTCAGAGCGTGACAGAGAGGTTTGAGGTGACCTGGG
  AAACAGTGTTGGTGAGCAGCCATGGAGCCGTGGTGGTCAAGTGTGACGGCCGAGGCAGCGGCTTCCAGGG
  AACCAAGCTCCTACAGGAAGTACGGAGGCGGCTAGGCTTCCTGGAGGAGAAGGACCAGATGGAAGCTGTA
  AGAACTATGCTGAAGGAGCAGTATATTGACAAGACTCGGGTAGCTGTGTTTGGGAAGGATTATGGTGGGT
  ACCTGAGCACTTACATCCTCCCAGCCAAGGGAGAAAATCAAGGTCAGACTTTCACCTGCGGCTCTGCGCT
  CTCTCCAATAACAGACTTCAAACTCTATGCCTCTGCATTTTCTGAGAGGTACCTTGGCCTCCATGGACTC
  GACAACAGAGCGTATGAGATGACCAAGCTGGCACACCGAGTGTCAGCACTGGAGGACCAGCAGTTCCTGA
  TCATCCACGCCACGGCCGATGAAAAAATCCATTTCCAGCACACAGCTGAGCTCATCACGCAGCTGATCAA
  GGGAAAGGCCAATTACAGCTTACAGATCTACCCAGATGAGAGTCACTACTTCCACAGCGTGGCGCTCAAG
  CAGCACCTGTCCAGGTCCATCATCGGCTTCTTTGTGGAATGTTTCAGAGTCCAAGATAAGCTACCAACAG
  CCACAGCAAAAGAGGAGGAGGAGGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210746 representing BC048383
  Red=Cloning site Green=Tags(s)
  
  MTTAKEPSASGKSVQQQDQELVGSNPPQRNWKGIAIALLVILVICSLIVTSVILLTPAEDTSLSQKKKVT
  VEDLFSEDFKIHDPEAKWISNKEFIYRERKGSVILRNVETNNSTVLIEGKKIESLRAIRYEISPDKEYVL
  FSYNVEPVYQHSHTGYYVLSKIPHGDPQSLDPPEVSNAKLQYAGWGPKGQQLIFIFENNIYYCAHVGKQA
  IRVVSTGKEGVIYNGLSDWLYEEEILKSHIAHWWSPDGTRLAYATINDSRVPLMELPTYTGSVYPTVKPY
  HYPKAGSENPSISLHVIGLNGPTHDLEMMPPDDPRMREYYITMVKWATSTKVAVTWLNRAQNVSILTLCD
  ATTGVCTKKHEDESEAWLHRQNEEPVFSKDGRKFFFVRAIPQGGRGKFYHITVSSSQPNSSNDNIQSITS
  GDWDVTKILSYDEKRNKIYFLSTEDLPRRRHLYSANTVDDFNRQCLSCDLVENCTYVSASFSHNMDFFLL
  KCEGPGVPTVTVHNTTDKRRMFDLEANEEVQKAINDRQMPKIEYRKIEVEDYSLPMQILKPATFTDTAHY
  PLLLVVDGTPGSQSVTERFEVTWETVLVSSHGAVVVKCDGRGSGFQGTKLLQEVRRRLGFLEEKDQMEAV
  RTMLKEQYIDKTRVAVFGKDYGGYLSTYILPAKGENQGQTFTCGSALSPITDFKLYASAFSERYLGLHGL
  DNRAYEMTKLAHRVSALEDQQFLIIHATADEKIHFQHTAELITQLIKGKANYSLQIYPDESHYFHSVALK
  QHLSRSIIGFFVECFRVQDKLPTATAKEEEEED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC048383
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048383, AAH48383
• RefSeq Size: 3389 bp
• RefSeq ORF: 2411 bp
• Locus ID: 13483
• MW: 124.2 kDa