Vcp (BC043053) Mouse Tagged ORF Clone

CAT#: MR210758

Vcp (Myc-DDK-tagged) - Mouse valosin containing protein (cDNA clone MGC:57954 IMAGE:6418662)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vcp
• Synonyms: p97, CDC48, 3110001E05
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210758 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCTGGAGCCGATTCAAAAGGTGATGATTTATCAACAGCCATTCTCAAACAGAAGAACCGACCCA
  ATCGGTTAATTGTTGATGAAGCCATCAATGAAGATAACAGCGTGGTGTCCTTGTCCCAGCCCAAGATGGA
  TGAACTGCAGTTGTTCCGAGGTGACACGGTGTTGCTAAAAGGAAAGAAAAGACGGGAAGCTGTATGCATT
  GTTCTTTCTGATGACACGTGTTCTGATGAGAAGATTCGAATGAATAGAGTTGTTCGGAATAACCTCCGAG
  TTCGCCTAGGAGATGTCATCAGCATCCAGCCATGCCCTGATGTAAAGTATGGCAAACGTATCCACGTTCT
  ACCCATCGATGACACAGTGGAAGGCATCACTGGCAATCTCTTTGAGGTATACCTTAAGCCGTACTTCCTG
  GAAGCTTATCGGCCCATCCGTAAAGGAGATATTTTTCTTGTCCGGGGTGGGATGCGTGCTGTGGAGTTCA
  AAGTTGTAGAGACAGATCCCAGCCCTTACTGTATTGTTGCTCCAGACACAGTGATCCACTGTGAGGGGGA
  GCCAATCAAGCGAGAGGATGAGGAGGAATCCTTGAATGAAGTAGGCTATGATGACATCGGTGGTTGCAGG
  AAGCAGCTAGCTCAGATAAAGGAGATGGTGGAGCTGCCACTGAGACATCCTGCGCTCTTTAAGGCGATTG
  GTGTAAAGCCTCCTCGGGGAATCTTGTTGTATGGGCCTCCTGGGACAGGGAAGACCCTGATTGCTCGAGC
  TGTGGCAAATGAAACTGGAGCCTTCTTCTTTCTGATCAATGGTCCTGAAATCATGAGCAAATTGGCTGGT
  GAGTCTGAGAGCAACCTTCGTAAAGCCTTTGAGGAAGCTGAAAAGAATGCTCCTGCTATCATCTTCATCG
  ATGAGCTTGATGCCATTGCACCCAAAAGAGAGAAAACTCATGGGGAAGTGGAGCGTCGCATCGTGTCTCA
  GTTGTTGACCCTCATGGATGGCCTAAAGCAGAGAGCACATGTGATAGTTATGGCAGCAACCAATAGACCC
  AACAGCATTGACCCAGCCCTACGGCGATTTGGTCGCTTTGACAGAGAGGTAGATATTGGAATACCTGATG
  CTACAGGACGTTTGGAGATTCTTCAGATCCATACCAAGAACATGAAACTGGCAGATGATGTGGACTTGGA
  ACAGGTAGCCAATGAGACTCATGGTCATGTTGGTGCTGATTTGGCAGCCCTATGTTCAGAGGCTGCTCTG
  CAGGCCATCCGGAAAAAAATGGACCTCATTGACCTAGAAGATGAGACCATTGATGCTGAGGTCATGAATT
  CCCTGGCAGTTACTATGGATGACTTCCGGTGGGCTTTGAGTCAAAGCAACCCATCAGCACTTCGGGAAAC
  TGTGGTAGAGGTGCCACAAGTAACCTGGGAAGATATTGGAGGCCTGGAGGATGTCAAACGTGAGCTTCAG
  GAGTTGGTTCAGTATCCTGTGGAACATCCAGACAAATTCCTCAAATTTGGCATGACTCCCTCCAAAGGCG
  TTCTTTTCTATGGACCTCCTGGCTGTGGGAAAACCTTACTGGCTAAAGCCATTGCTAATGAATGCCAGGC
  CAACTTCATCTCCATCAAGGGTCCTGAGCTGCTTACCATGTGGTTTGGGGAATCTGAGGCCAATGTCCGG
  GAAATTTTTGACAAGGCACGGCAAGCTGCCCCCTGTGTACTCTTCTTTGATGAGTTAGATTCAATTGCCA
  AGGCTCGAGGTGGTAATATTGGAGATGGTGGTGGAGCTGCTGACCGAGTCATCAATCAGATCCTGACAGA
  AATGGATGGCATGTCTACAAAAAAGAATGTGTTTATCATTGGAGCTACCAACAGGCCTGACATCATTGAT
  CCTGCTATCCTAAGACCTGGCCGTCTAGATCAGCTCATTTATATCCCACTTCCTGATGAGAAGTCCCGTG
  TTGCCATCCTAAAAGCCAATCTGCGAAAGTCCCCAGTTGCCAAGGATGTGGATTTGGAGTTCCTGGCTAA
  GATGACTAATGGCTTTTCTGGAGCTGATTTGACAGAAATTTGCCAACGGGCTTGTAAACTGGCCATTCGT
  GAATCTATTGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAATCCATCGGCTATGGAGGTAGAAG
  AGGACGATCCAGTGCCTGAGATCCGCAGAGATCACTTTGAGGAAGCCATGCGTTTTGCCCGACGTTCTGT
  CAGCGATAATGACATTCGGAAGTATGAAATGTTCGCCCAGACACTGCAGCAGAGTCGAGGTTTTGGCAGC
  TTCAGATTCCCTTCAGGGAACCAGGGTGGAGCTGGTCCCAGCCAGGGCAGTGGAGGTGGCACAGGTGGCA
  GTGTGTACACAGAAGACAATGACGATGACCTGTATGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210758 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCI
  VLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFL
  EAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCR
  KQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAG
  ESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRP
  NSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAAL
  QAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQ
  ELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVR
  EIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIID
  PAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIR
  ESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGS
  FRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC043053
• ORF Size: 2418 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC043053.1
• RefSeq Size: 2898
• RefSeq ORF: 2420
• Locus ID: 269523
• MW: 89.3 kDa