Fgfr4 (NM_008011) Mouse Tagged ORF Clone

CAT#: MR210769

Fgfr4 (Myc-DDK-tagged) - Mouse fibroblast growth factor receptor 4 (Fgfr4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgfr4
• Synonyms: Fgfr-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210769 representing NM_008011
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGCTCTTGGCCCTGTTGAGCATCTTTCAGGGGACACCAGCTTTGTCCCTTGAGGCCTCTGAGG
  AAATGGAGCAGGAGCCCTGCCTAGCCCCAATCCTGGAGCAGCAAGAGCAGGTGTTGACGGTGGCCCTGGG
  GCAGCCTGTGAGGCTGTGCTGTGGGCGCACCGAGCGTGGTCGTCACTGGTACAAAGAGGGCAGCCGCCTA
  GCATCTGCTGGGCGAGTACGGGGTTGGAGAGGCCGCCTGGAGATCGCCAGCTTCCTTCCTGAGGATGCTG
  GCCGATACCTCTGCCTGGCCCGTGGCTCCATGACCGTCGTACACAATCTTACGTTGCTTATGGATGACTC
  CTTAACCTCCATCAGTAATGATGAAGACCCCAAGACACTCAGCAGCTCCTCGAGTGGTCATGTCTACCCA
  CAGCAAGCACCCTACTGGACACACCCCCAACGCATGGAGAAGAAACTGCATGCAGTGCCTGCCGGGAATA
  CTGTCAAATTCCGCTGTCCAGCTGCAGGGAACCCCATGCCTACCATCCACTGGCTCAAGGATGGACAGGC
  CTTCCACGGGGAGAATCGTATTGGAGGCATTCGGCTGCGCCACCAACACTGGAGCCTGGTGATGGAAAGT
  GTGGTACCCTCGGACCGTGGCACATACACATGCCTTGTGGAGAACTCTCTGGGTAGCATTCGCTACAGCT
  ATCTCCTGGATGTGCTGGAGCGGTCCCCGCACCGGCCCATCCTGCAGGCGGGGCTCCCAGCCAACACCAC
  AGCTGTGGTTGGCAGCGATGTGGAGCTACTCTGCAAGGTGTACAGCGACGCCCAGCCCCACATACAGTGG
  CTGAAACACGTCGTCATCAACGGCAGCAGCTTCGGCGCCGACGGTTTCCCCTACGTACAAGTCCTGAAGA
  CAACAGACATCAATAGCTCGGAGGTAGAGGTCTTGTATCTGAGGAACGTGTCCGCTGAGGATGCAGGAGA
  GTATACCTGTCTGGCGGGCAACTCCATCGGCCTTTCCTACCAGTCAGCGTGGCTCACGGTGCTGCCAGAG
  GAAGACCTCACGTGGACAACAGCAACCCCTGAGGCCAGATACACAGATATCATCCTGTATGTATCAGGCT
  CACTGGTTCTGCTTGTGCTCCTGCTGCTGGCCGGGGTGTATCATCGGCAAGTCATCCGTGGCCACTACTC
  TCGCCAGCCTGTCACTATACAAAAGCTGTCCCGTTTCCCTTTGGCCCGACAGTTCTCTTTGGAGTCGAGG
  TCCTCTGGCAAGTCAAGTTTGTCCCTGGTGCGAGGTGTCCGTCTCTCCTCCAGCGGCCCGCCCTTGCTCA
  CGGGCCTTGTGAATCTAGACCTGCCTCTCGATCCGCTTTGGGAATTCCCCCGGGACAGGTTGGTGCTCGG
  AAAGCCCCTGGGTGAGGGCTGCTTTGGGCAAGTGGTTCGTGCAGAGGCCTTTGGTATGGATCCCTCCCGG
  CCCGACCAAACCAGCACCGTGGCTGTGAAGATGCTGAAAGACAATGCCTCCGACAAGGATTTGGCAGACC
  TGGTCTCCGAGATGGAGGTGATGAAGCTAATCGGAAGACACAAGAACATCATCAACCTGCTGGGTGTCTG
  CACTCAGGAAGGGCCCCTGTACGTGATTGTGGAATGTGCCGCCAAGGGAAATCTTCGGGAATTCCTCCGT
  GCCCGGCGCCCCCCAGGCCCTGATCTCAGCCCTGATGGACCTCGGAGCAGCGAAGGACCACTCTCCTTCC
  CGGCCCTAGTCTCCTGTGCCTACCAGGTGGCCCGAGGCATGCAGTATCTGGAGTCTCGGAAGTGCATCCA
  CCGGGACCTGGCTGCCCGAAATGTGCTGGTGACCGAGGATGATGTGATGAAGATCGCTGACTTTGGGCTG
  GCACGTGGTGTCCACCACATTGACTACTATAAGAAAACCAGCAACGGCCGCCTGCCAGTCAAATGGATGG
  CTCCAGAGGCGTTGTTCGACCGTGTGTACACACACCAGAGTGACGTGTGGTCTTTCGGGATCCTGCTGTG
  GGAAATCTTCACCCTCGGGGGCTCCCCATACCCTGGCATTCCGGTGGAGGAGCTCTTCTCACTGCTGCGA
  GAGGGGCACAGGATGGAGCGGCCCCCAAACTGCCCCTCAGAGCTGTATGGGCTAATGAGGGAGTGCTGGC
  ACGCAGTCCCATCTCAGAGGCCTACTTTTAAGCAGCTGGTGGAAGCTCTGGACAAGGTCCTGCTGGCTGT
  CTCTGAAGAGTACCTTGACCTCCGCCTGACCTTTGGACCCTTTTCTCCCTCCAATGGGGATGCCAGCAGC
  ACCTGCTCCTCCAGTGACTCGGTTTTCAGCCACGACCCTTTGCCCCTCGAGCCAAGCCCCTTCCCTTTCT
  CTGACTCGCAGACGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210769 representing NM_008011
  Red=Cloning site Green=Tags(s)
  
  MWLLLALLSIFQGTPALSLEASEEMEQEPCLAPILEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRL
  ASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLLMDDSLTSISNDEDPKTLSSSSSGHVYP
  QQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKDGQAFHGENRIGGIRLRHQHWSLVMES
  VVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQW
  LKHVVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPE
  EDLTWTTATPEARYTDIILYVSGSLVLLVLLLLAGVYHRQVIRGHYSRQPVTIQKLSRFPLARQFSLESR
  SSGKSSLSLVRGVRLSSSGPPLLTGLVNLDLPLDPLWEFPRDRLVLGKPLGEGCFGQVVRAEAFGMDPSR
  PDQTSTVAVKMLKDNASDKDLADLVSEMEVMKLIGRHKNIINLLGVCTQEGPLYVIVECAAKGNLREFLR
  ARRPPGPDLSPDGPRSSEGPLSFPALVSCAYQVARGMQYLESRKCIHRDLAARNVLVTEDDVMKIADFGL
  ARGVHHIDYYKKTSNGRLPVKWMAPEALFDRVYTHQSDVWSFGILLWEIFTLGGSPYPGIPVEELFSLLR
  EGHRMERPPNCPSELYGLMRECWHAVPSQRPTFKQLVEALDKVLLAVSEEYLDLRLTFGPFSPSNGDASS
  TCSSSDSVFSHDPLPLEPSPFPFSDSQTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008011
• ORF Size: 2397 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008011.2, NP_032037.2
• RefSeq Size: 3146 bp
• RefSeq ORF: 2400 bp
• Locus ID: 14186
• MW: 89 kDa