Cd177 (NM_026862) Mouse Tagged ORF Clone

CAT#: MR210799

Cd177 (Myc-DDK-tagged) - Mouse CD177 antigen (Cd177)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd177
• Synonyms: 1190003K14Rik; Pdp3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210799 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTCTATACCAGTGCTGACCCTTCTGGGGGTCACGGCCTTGCTACCCTGTGTCCCAGCTCTGACCT
  GCCAGAAAAGCAGCGCACAGGCTGTGAGGAATGTGGCAGAGCTGCCCCTCAGGTGGTGGGGAGCTGGTGA
  GAAAACCTGCGAGGTTAGCGAGGGTTGCCAAGACTTGATAATGCTCCTGTATAATGGACCCAAGGTCAAC
  TTGGTGATCATCAAGGGCTGCACCGAGGTTGAGGACCAAGAGCCGAAGGTGATCTGGCTCAGGACAGGCC
  CTGGGCTCTCTGTGGTGTCCTACACCCGTGTGTGTCGCCATGGTGACCTCTGCAATGATGTGAACAGCAC
  TAAGATCCTTGAGGAGCTACCTACACCCACAGTTCCAGGGTCCCTGCGCTGCCCACTCTGCCTTTCTAAT
  GACAGCTGTGAGAATGCACCGGAGCAGGTCTGCCCTGTGGGAAGCACACACTGCTACGATGGAGTCCTCA
  GGCTCAGGGGAGATGGCATCAGGACCAATCTCAAGGTGCAGGGCTGCATGGCCCAGCCAGACTGCAACCT
  GCTTAATGGCACCCAGGCGATTGGGACCTTGTATATGAGCGAAAACTGTGATCTTATAGGTCCACAGGCT
  CTGGATTGCAATAGTGGGAGCTTGGAAACTGTGAGGAATGTATCAGATCTGCACTTGAGCTGGACGACTG
  GCTGGCAAACCTGTGAGGCTGGCGAGGGGTGTTATGAAACAGTGATGCTAATACAAAATGGACATGAATT
  TCACATGGTTCTCACTAAGGGATGTACTAGGGATATGAACAAAAAGGCTCGGCTCACCAGGCATAGAACA
  GGCCCAGGGATCTCCATCGTCTCCTACGTGCATGTGTGCCGCGACAGGGACTTCTGTAATGACCTGTCTA
  CAACAGACCCTCTTTGGACCCCGCCCCCTGACACAGAGCTAGGGACCCTGCGCTGCCGACACTGCCTTTC
  AACCGGCAGCTGTGTGAGTGCATCCGAGCTGGTCTGCCCCGCAGGCAGCACACACTGCTACAGTGGAGTC
  CTCAGCCTCAGGGGAGGAGGGGTCATTTCTGATCTGAAGGTACAGGGATGCATATCGCAGTCCCAGCCAG
  GATGCAACCTGCTCAACGGTACCCAGACAATCGGACCCGTGGATGTGCGTGAGGACTGCGGTCTTCAGTT
  AGATGCTCTGAAATGCCAGCATGGGACGCTGAAGACCATCCAGGATATATCGAAGCTGCCTCTCCAGTGG
  ACGGCTGGCCAGAAAATCTGTAATGTGGGTGAAGGCTGCCAAGACACACTGATGTTGATAGAGAACGGAG
  AGCAGGTGAACTTGGTTCTCACGAAAGGCTGCACTACCGCAAAGGACCAAGAGGCCAAAGTCACGGAGCA
  CAGAACTGGACCAGGGCTGTCTGTCACCTCCTACACCCGAGTGTGCCGTAAAAAAGACTTCTGCAATGAC
  CTGTCTACCACAGCCCCTCTCTGGGCTCCACCTCCAGTGACAGCCCCAGGGACCACTCGCTGCCCTCTCT
  GCTTTTCTGAACAAGCCTGTGAGAATGCACCGGAGCAGGTCTGCCCTGCAGGCAGCACACACTGCTACAG
  TGGAGTCCTCAGCCTCAGGGGAGGAGGGATCATCTCTGATCTGAAGGTGCAGGGCTGTATGTCCCAGCCA
  GGATGCAACCTGCTCAACGGTACCCAGACAATCGGACCCGTGGATGTGAGCGAGCGCTGCAGTCCTCCGT
  CAGAAACAACAGAGTTGTCCTGTTACAGGGGTGTGATGTTTGAGCTTGGCAATGGCTTTGCGGAGGAACC
  TGTCAAGTGGACGGCACCAGGGTCTCAGGTGTGTGCACCTGATGAGATTTGTCAAGAGACGCTGCTGCTC
  ATAGACGTAGGACAAAAATCAGCCTTCCTGGGGAGTAAAGGCTGCAGCAGTCCTGGGGCGCAGGACAATA
  TTGGTGTCTCCATATTCTCCCGGCTCCCTGGGATGCTGGTAGCTTCCTATACCAAATTCTGTTCTTCCCA
  CCTGTGCAATGGAGCCGACAGCAGCAGTGTCCTTCTAAGCATCCTCCCTCGTCCAGATGTTCCTCCCCCA
  GGAGATGTGCAGTGCCCCATGTGTGTAGAGTTATTTGGATCCTGCAAGAGCACTGACTCTGTCACCTGCC
  CTAGGGGTGCCACTCACTGTTATAAAGGTGACATTGCACTACAGGGAGGTGGACTGACTACCAGAGTGAG
  CATTCAGGGGTGCATGGCCCCACCTATCAAACCTTTACTGGGTGACTCCAAAACAATCGGTATCTTCTCG
  GCAGAGGAGAGCTCTAACTATCGACATGAGGATGATGTTACCTCGGCCCCTTCCCTGGCCTGGACCTTAC
  GGCTATCGGCCTGGATGTTAGGGCTATCGGCTCTTCTCAGCTCTTTGTATGCTGGGATCTGTCCTCTCTG
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210799 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNSIPVLTLLGVTALLPCVPALTCQKSSAQAVRNVAELPLRWWGAGEKTCEVSEGCQDLIMLLYNGPKVN
  LVIIKGCTEVEDQEPKVIWLRTGPGLSVVSYTRVCRHGDLCNDVNSTKILEELPTPTVPGSLRCPLCLSN
  DSCENAPEQVCPVGSTHCYDGVLRLRGDGIRTNLKVQGCMAQPDCNLLNGTQAIGTLYMSENCDLIGPQA
  LDCNSGSLETVRNVSDLHLSWTTGWQTCEAGEGCYETVMLIQNGHEFHMVLTKGCTRDMNKKARLTRHRT
  GPGISIVSYVHVCRDRDFCNDLSTTDPLWTPPPDTELGTLRCRHCLSTGSCVSASELVCPAGSTHCYSGV
  LSLRGGGVISDLKVQGCISQSQPGCNLLNGTQTIGPVDVREDCGLQLDALKCQHGTLKTIQDISKLPLQW
  TAGQKICNVGEGCQDTLMLIENGEQVNLVLTKGCTTAKDQEAKVTEHRTGPGLSVTSYTRVCRKKDFCND
  LSTTAPLWAPPPVTAPGTTRCPLCFSEQACENAPEQVCPAGSTHCYSGVLSLRGGGIISDLKVQGCMSQP
  GCNLLNGTQTIGPVDVSERCSPPSETTELSCYRGVMFELGNGFAEEPVKWTAPGSQVCAPDEICQETLLL
  IDVGQKSAFLGSKGCSSPGAQDNIGVSIFSRLPGMLVASYTKFCSSHLCNGADSSSVLLSILPRPDVPPP
  GDVQCPMCVELFGSCKSTDSVTCPRGATHCYKGDIALQGGGLTTRVSIQGCMAPPIKPLLGDSKTIGIFS
  AEESSNYRHEDDVTSAPSLAWTLRLSAWMLGLSALLSSLYAGICPLC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026862
• ORF Size: 2454 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026862.3, NP_081138.2
• RefSeq Size: 2733 bp
• RefSeq ORF: 2454 bp
• Locus ID: 68891
• MW: 87.1 kDa