Robo2 (BC055333) Mouse Tagged ORF Clone

CAT#: MR210811

  • TrueORF®

Robo2 (Myc-DDK-tagged) - Mouse roundabout homolog 2 (Drosophila) (cDNA clone MGC:65719 IMAGE:6409283)


  "BC055333" in other vectors (4)


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Reconstitution Protocol

USD 860.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Robo2
Synonyms mKIAA1568
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210811 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCCTCTGATGTTTACACTATTATTGCTCTTTGGATTTCTCTGCATTCAGATTGATGGATCTCGTC
TTCGTCAAGAAGACTTTCCCCCCAGGATCGTAGAACACCCTTCTGATGTCATCGTCTCCAAGGGAGAGCC
CACCACTCTGAACTGTAAAGCAGAGGGCCGACCCACCCCCACCATTGAATGGTACAAGGATGGTGAGAGG
GTGGAGACAGACAAGGATGATCCCAGGTCCCACAGAATGCTTCTGCCCAGCGGATCTTTATTCTTTTTGC
GAATTGTTCATGGGCGCAGAAGTAAACCGGACGAAGGGAGTTACGTTTGTGTTGCAAGGAACTATCTTGG
TGAAGCAGTGAGTCGAAATGCATCTCTGGAAGTGGCATTATTGCGAGATGACTTCCGGCAAAACCCCACA
GATGTGGTAGTCGCAGCTGGAGAGCCTGCAATCTTGGAGTGCCAGCCACCACGGGGACACCCAGAACCAA
CCATCTACTGGAAAAAGGACAAAGTCCGAATTGATGACAAGGAAGAGAGAATAAGTATCCGTGGTGGGAA
GCTGATGATCTCTAATACTAGGAAAAGCGATGCTGGCATGTACACCTGTGTGGGAACCAATATGGTGGGA
GAAAGGGACAGCGACCCTGCAGAGCTCACTGTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACC
AGGTGGTGCTAGAGGAAGAAGCTGTAGAATTCCGTTGTCAGGTCCAAGGAGATCCCCAGCCAACGGTGAG
GTGGAAAAAAGATGATGCAGACTTGCCGAGAGGAAGGTATGATATCAAAGATGACTACACGCTGAGAATT
AAAAAGGCCATGAGTACTGATGAAGGTACCTATGTGTGTATTGCTGAGAATCGGGTGGGAAAAGTGGAAG
CCTCTGCTACCCTCACTGTCCGAGTTCGCCCTGTTGCTCCTCCACAGTTTGTGGTTAGGCCAAGAGATCA
GATCGTTGCTCAAGGCCGAACAGTGACATTCCCCTGTGAAACTAAAGGAAACCCACAGCCAGCTGTTTTT
TGGCAGAAAGAAGGCAGCCAGAACCTACTTTTCCCGAATCAACCTCAGCAGCCCAACAGCCGATGTTCAG
TGTCGCCCACGGGGGACCTCACCATCACCAACATCCAGCGTTCAGATGCGGGTTACTACATCTGCCAGGC
CCTAACCGTGGCAGGAAGCATTTTAGCTAAAGCACAGTTGGAAGTTACTGACGTTTTGACAGATAGACCT
CCACCCATAATCTTGCAAGGACCAATAAACCAAACACTTGCAGTAGACGGTACAGCATTGTTGAAGTGTA
AAGCCACTGGTGAGCCTCTGCCTGTAATTAGCTGGCTAAAGGAGGGCTTTACTTTTCTGGGGAGAGATCC
AAGAGCCACGATCCAAGACCAAGGAACACTGCAGATTAAGAATTTACGGATATCTGATACTGGCACTTAT
ACTTGTGTGGCTACAAGTTCCAGTGGAGAGACTTCCTGGAGTGCAGTGCTGGATGTAACAGAATCTGGAG
CAACAATCAGTAAAAATTATGATATGAATGACCTCCCGGGACCACCATCCAAACCTCAGGTCACTGATGT
TTCTAAGAACAGTGTCACCTTATCCTGGCAGCCAGGTACACCTGGCGTTCTTCCTGCAAGCGCGTATATC
ATTGAGGCTTTCAGCCAATCGGTGAGCAATAGCTGGCAGACAGTGGCAAACCATGTTAAGACAACTCTGT
ATACAGTAAGGGGGCTGAGGCCCAACACAATCTACTTGTTTATGGTCAGAGCGATCAACCCACAAGGTCT
CAGTGATCCAAGTCCTATGTCGGATCCTGTACGCACACAAGATATCAGCCCCCCAGCACAAGGAGTGGAC
CACAGACAGGTGCAGAAGGAATTAGGTGATGTCGTTGTTCGTCTCCATAATCCAGTTGTCCTGACACCTA
CAACTGTTCAAGTCACATGGACGGTGGACCGACAACCCCAGTTTATTCAGGGCTACAGAGTGATGTACCG
TCAGACTTCGGGACTACAAGCCTCAACTGTGTGGCAGAATCTAGACGCCAAAGTCCCGACTGAGAGGAGT
GCTGTCCTTGTGAATTTGAAAAAGGGGGTGACTTATGAAATTAAAGTCCGGCCGTATTTTAACGAGTTCC
AAGGAATGGACAGTGAATCGAAAACAGTCCGAACCACTGAGGAAGCCCCAAGTGCCCCTCCCCAGTCTGT
CACTGTGCTGACAGTTGGAAGTCACAACAGCACAAGCATCAGTGTTTCCTGGGATCCTCCACCAGCCGAC
CACCAGAATGGAATTATTCAGGAATATAAGATCTGGTGTCTGGGAAACGAAACGCGATTCCATATCAATA
AAACGGTGGATGCAGCCATTCGCTCTGTAGTAATAGGTGCATCCACCAGCCGACCACCAGAATGGAATTA
TTCAGGAATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210811 protein sequence
Red=Cloning site Green=Tags(s)

MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGER
VETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPT
DVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVG
ERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRI
KKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVF
WQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRP
PPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTY
TCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYI
IEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVD
HRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERS
AVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPAD
HQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGASTSRPPEWNYSGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC055333
ORF Size 2460 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC055333, AAH55333
RefSeq Size 4346 bp
RefSeq ORF 2462 bp
Locus ID 268902
MW 91 kDa

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