Gpam (NM_008149) Mouse Tagged ORF Clone

CAT#: MR210843

Gpam (Myc-DDK-tagged) - Mouse glycerol-3-phosphate acyltransferase, mitochondrial (Gpam), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpam
• Synonyms: GPAT; GPAT-1; GPAT1; P90
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210843 representing NM_008149
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGTCTTCAGTGACAGTTGGCACAATAGACGTTTCTTATCTGCCCAGTTCATCGGAATACAGCC
  TTGGCCGATGTAAACACACCAGTGAGGACTGGGTTGACTGTGGGTTCAAACCTACCTTCTTCAGATCTGC
  AACACTGAAATGGAAGGAGAGCCTTATGAGCCGGAAGAGGCCCTTCGTGGGAAGGTGCTGCTATTCCTGC
  ACGCCACAGAGCTGGGAAAGGTTTTTCAACCCCAGTATCCCATCTCTGGGTTTGCGGAATGTTATTTATA
  TCAATGAAACGCACACAAGGCACAGAGGATGGCTGGCGAGACGGCTGTCTTACATCCTTTTTGTTCAAGA
  GCGAGACGTCCATAAGGGCATGTTTGCCACCAGTGTTACTGAGAATGTACTGAGCAGCAGCAGAGTCCAA
  GAGGCAATTGCTGAAGTGGCTGCGGAGTTGAACCCAGATGGATCTGCCCAGCAGCAGTCCAAAGCCATCC
  AGAAGGTGAAAAGGAAAGCCAGGAAGATCCTCCAGGAGATGGTCGCCACCGTCTCCCCAGGGATGATCAG
  GCTGACTGGCTGGGTGTTACTAAAGCTCTTCAACAGCTTCTTCTGGAACATTCAGATTCACAAGGGTCAA
  CTCGAGATGGTCAAGGCTGCAACTGAGACGAACCTGCCGCTCTTGTTTCTGCCGGTGCACAGATCCCACA
  TTGACTACCTGTTGCTCACCTTCATCCTCTTTTGCCACAACATCAAGGCGCCGTACATCGCCTCGGGCAA
  TAATCTCAACATCCCCGTCTTCAGTACCTTGATTCACAAGCTTGGGGGCTTTTTCATAAGACGGAGGCTC
  GATGAAACCCCAGATGGACGCAAAGACATTCTGTACAGAGCGTTGCTCCATGGGCATGTAGTTGAACTCC
  TCCGACAGCAGCAGTTCCTGGAGATCTTCCTGGAAGGCACCCGCTCCCGCAGTGGCAAGACCTCCTGTGC
  CCGGGCAGGGCTCCTCTCAGTGGTAGTGGATACTCTGTCGTCCAACACCATCCCCGACATCCTCGTCATA
  CCCGTGGGCATCTCGTATGATCGCATAATCGAAGGTCACTACAATGGCGAACAGTTGGGAAAGCCCAAGA
  AGAACGAGAGCCTCTGGAGTGTGGCGAGAGGCGTTATCAGAATGCTGCGGAAAAACTACGGCTACGTCCG
  AGTGGATTTTGCACAGCCATTTTCCTTGAAGGAATATTTAGAAGGCCAGAGTCAGAAACCTGTATCTGCC
  CCCCTTTCTCTGGAGCAAGCACTGTTACCAGCGATCCTTCCTTCAAGACCGAATGATGTTGCTGATGAAC
  ATCAAGACCTATCCAGTAACGAGTCCAGAAACCCAGCAGACGAAGCCTTCCGACGAAGGCTGATTGCAAA
  CCTGGCTGAGCACATTCTCTTCACCGCCAGCAAGTCCTGCGCTATCATGTCCACCCACATTGTGGCCTGT
  CTGCTCCTCTACAGACACAGGCAGGGAATCCATCTCTCCACGCTTGTGGAAGACTTCTTTGTGATGAAGG
  AGGAAGTCCTAGCTCGCGATTTCGACCTAGGCTTCTCCGGGAATTCAGAAGATGTCGTCATGCATGCTAT
  TCAGCTTCTGGGGAACTGTGTCACAATCACCCACACGAGCAGGAAAGATGAGTTTTTTATTACTCCCAGC
  ACAACTGTCCCGTCAGTCTTTGAACTCAACTTCTACAGCAATGGCGTACTTCATGTGTTCATCATGGAAG
  CCATCATAGCTTGCAGCATCTATGCAGTCCTGAATAAGAGGTGCTCTGGAGGGTCCGCTGGAGGCCTCGG
  CAACCTGATCAGCCAGGAGCAGCTGGTGAGGAAGGCCGCCAGCCTGTGCTACCTTCTCTCTAACGAAGGT
  ACCATTTCTCTGCCCTGCCAGACTTTTTACCAAGTTTGTCATGAGACAGTTGGCAAGTTCATCCAGTATG
  GCATTCTCACAGTGGCAGAGCAAGATGACCAGGAAGATGTCAGTCCTGGCCTTGCAGAACAGCAGTGGGA
  CAAGAAGCTTCCGGAACTGAACTGGAGAAGTGACGAGGAAGATGAAGACAGTGACTTTGGTGAGGAGCAG
  CGAGATTGCTATCTCAAGGTGAGCCAGTCCAAGGAGCACCAGCAATTCATCACCTTTCTTCAGAGGCTTC
  TAGGTCCCCTGCTAGAAGCCTACAGCTCTGCTGCCATCTTTGTCCACAACTTCAGCGGTCCAGTTCCCGA
  GTCTGAGTACCTGCAGAAACTGCACAGGTACCTTATCACCAGGACGGAAAGGAACGTTGCCGTGTACGCT
  GAGAGTGCCACATACTGTCTCGTGAAGAACGCTGTGAAAATGTTTAAGGACATCGGGGTTTTCAAAGAGA
  CCAAGCAAAAGCGAGTGTCTGTTCTAGAACTGAGCAGTACTTTCCTACCTCAGTGCAACCGGCAGAAACT
  CCTAGAGTATATTCTGAGTTTTGTGGTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210843 representing NM_008149
  Red=Cloning site Green=Tags(s)
  
  MEESSVTVGTIDVSYLPSSSEYSLGRCKHTSEDWVDCGFKPTFFRSATLKWKESLMSRKRPFVGRCCYSC
  TPQSWERFFNPSIPSLGLRNVIYINETHTRHRGWLARRLSYILFVQERDVHKGMFATSVTENVLSSSRVQ
  EAIAEVAAELNPDGSAQQQSKAIQKVKRKARKILQEMVATVSPGMIRLTGWVLLKLFNSFFWNIQIHKGQ
  LEMVKAATETNLPLLFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPVFSTLIHKLGGFFIRRRL
  DETPDGRKDILYRALLHGHVVELLRQQQFLEIFLEGTRSRSGKTSCARAGLLSVVVDTLSSNTIPDILVI
  PVGISYDRIIEGHYNGEQLGKPKKNESLWSVARGVIRMLRKNYGYVRVDFAQPFSLKEYLEGQSQKPVSA
  PLSLEQALLPAILPSRPNDVADEHQDLSSNESRNPADEAFRRRLIANLAEHILFTASKSCAIMSTHIVAC
  LLLYRHRQGIHLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRKDEFFITPS
  TTVPSVFELNFYSNGVLHVFIMEAIIACSIYAVLNKRCSGGSAGGLGNLISQEQLVRKAASLCYLLSNEG
  TISLPCQTFYQVCHETVGKFIQYGILTVAEQDDQEDVSPGLAEQQWDKKLPELNWRSDEEDEDSDFGEEQ
  RDCYLKVSQSKEHQQFITFLQRLLGPLLEAYSSAAIFVHNFSGPVPESEYLQKLHRYLITRTERNVAVYA
  ESATYCLVKNAVKMFKDIGVFKETKQKRVSVLELSSTFLPQCNRQKLLEYILSFVVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008149
• ORF Size: 2481 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008149.1, NM_008149.2, NM_008149.3, NP_032175.2
• RefSeq Size: 3852
• RefSeq ORF: 2484
• Locus ID: 14732
• MW: 94.2 kDa